ORP150/HSP12A Antibody

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and human breast cancer tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ORP150/HSP12A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5558) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ORP150/HSP12A at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

ORP150/HSP12A Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ORP150/HSP12A
  Met695-Leu994
  Accession # Q9Y4L1
• Specificity: Detects human ORP150/HSP12A in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) HSP-70, rhHSPA-2, and rhHSPH-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HYOU1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ORP150/HSP12A Antibody

• 150 kDa oxygen-regulated protein
• 170 kDa glucose-regulated protein
• DKFZp686N08236
• FLJ97572
• glucose-regulated protein 170
• Grp170
• GRP-170
• HSP12A
• HYOU1
• hypoxia up-regulated 1
• hypoxia up-regulated protein 1
• ORP150
• ORP-150
• ORP150FLJ94899
• oxygen regulated protein (150kD)

Background

ORP150 (Oxygen-related protein of 150 kDa) is a member of the heat shock protein 70 family of molecules. Although the predicted MW is 111 kDa, it runs at 150 kDa in SDS-PAGE. The discrepancy may not be anomalous, since it is secreted into the ER lumen and likely to be glycosylated and/or phosphorylated. ORP150 is highly expressed in cells involved in secretion such as pancreatic acinar cells and hepatocytes. Mature human ORP150 is 967 amino acids (aa) in length. It contains two HSP70 signatures (aa 230‑243 and 380‑394) and an ER-retention signal (aa 996‑999). There are multiple isoforms. Three show alternate start sites at Met88, Met152, and a Met 44 aa upstream of the standard start site. Four isoforms also show deletions of aa 148‑958, 51‑59, 603‑664 and 164‑507, respectively. There is one 44 aa substitution for aa 603‑999. Over aa 1‑994, human ORP150 shares 92% aa identity with mouse ORP150.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ORP150/HSP12A Antibody (AF5558)(1)

Publication using AF5558

• Pillay S, Zou W, Cheng F et al. AAV serotypes have distinctive interactions with domains of the cellular receptor AAVR J. Virol. 2017-07-05 [PMID: 28679762]

Bioinformatics

• Gene Symbol: HYOU1
• Uniprot:
  • Q9Y4L1()