ORP150/HSP12A Antibody (JG39-11)

Western Blot: ORP150/HSP12A Antibody (JG39-11) [NBP2-76858] - Western blot analysis of ORP150 on mouse prostate tissue lysate using anti-ORP150 antibody at 1/5,000 dilution.

Immunohistochemistry: ORP150/HSP12A Antibody (JG39-11) [NBP2-76858] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ORP150 antibody. Counter stained with hematoxylin.

Flow Cytometry: ORP150/HSP12A Antibody (JG39-11) [NBP2-76858] - Flow cytometric analysis of HepG2 cells with ORP150 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunohistochemistry: ORP150/HSP12A Antibody (JG39-11) [NBP2-76858] - Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-ORP150 antibody. Counter stained with hematoxylin.

Immunohistochemistry: ORP150/HSP12A Antibody (JG39-11) [NBP2-76858] - Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ORP150 antibody. Counter stained with hematoxylin.

Immunohistochemistry: ORP150/HSP12A Antibody (JG39-11) [NBP2-76858] - Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-ORP150 antibody. Counter stained with hematoxylin.

Product Discontinued

ORP150/HSP12A Antibody (JG39-11) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human ORP150/HSP12A aa 1-190 / 999. (SwissProt: Q9Y4L1 Human; SwissProt: Q9JKR6 Mouse)
• Localization: Endoplasmic reticulum.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HYOU1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:5000-1:20000
• Theoretical MW: 111 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ORP150/HSP12A Antibody (JG39-11)

• 150 kDa oxygen-regulated protein
• 170 kDa glucose-regulated protein
• DKFZp686N08236
• FLJ97572
• glucose-regulated protein 170
• Grp170
• GRP-170
• HSP12A
• HYOU1
• hypoxia up-regulated 1
• hypoxia up-regulated protein 1
• ORP150
• ORP-150
• ORP150FLJ94899
• oxygen regulated protein (150kD)

Background

HYOU1 is encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment is found at the 5' end of the first exon, and is involved in stress-dependent induction. A transcript that is produced from a downstream start site is preferentially induced by hypoxia, resulting in the accumulation of this protein in the endoplasmic reticulum (ER). The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HYOU1