Ornithine Decarboxylase Antibody (OTI1G6)

Western Blot: Ornithine Decarboxylase Antibody (1G6) [NBP2-02644] Analysis of extracts (35ug) from 9 different cell lines by using anti-Ornithine Decarboxylase monoclonal antibody.

Immunocytochemistry/Immunofluorescence: Ornithine Decarboxylase Antibody (1G6) [NBP2-02644] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Ornithine Decarboxylase.

Flow Cytometry: Ornithine Decarboxylase Antibody (1G6) [NBP2-02644] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Ornithine Decarboxylase antibody, and then analyzed by flow cytometry.

Western Blot: Ornithine Decarboxylase Antibody (1G6) [NBP2-02644] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Ornithine Decarboxylase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Ornithine Decarboxylase.

Flow Cytometry: Ornithine Decarboxylase Antibody (1G6) [NBP2-02644] - Analysis of Jurkat cells, using anti-Ornithine Decarboxylase antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, Flow, ICC/IF
• Clone: OTI1G6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.57 mg/ml

Ornithine Decarboxylase Antibody (OTI1G6) Summary

• Description: Novus Biologicals Mouse Ornithine Decarboxylase Antibody (OTI1G6) (NBP2-02644) is a monoclonal antibody validated for use in WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human ODC1 (NP_002530) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ODC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500-2000
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.57 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Ornithine Decarboxylase Antibody (OTI1G6)

• ODC
• ODC1
• ODCEC 4.1.1.17
• ornithine decarboxylase 1
• ornithine decarboxylase

Background

This gene encodes the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ODC1
• Entrez:
  • 4953(Human)