Oct-3/4 Antibody (240408) [Unconjugated]

Images

 
Western blot shows lysates of BG01V human embryonic stem cells and NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Oct‑3/4 Monoclonal Antibody ...read more
Oct‑3/4 was detected in immersion fixed BG01V human embryonic stem cells using Rat Anti-Human/Mouse Oct‑3/4 Monoclonal Antibody (Catalog # MAB1759) at 10 µg/mL for 3 hours at room temperature. Cells were stained ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, CyTOF-ready, Flow, ICC/IF
Clone
240408
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Oct-3/4 Antibody (240408) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Oct‑3/4
Met1-Asn265 (Met262Leu)
Accession # Q01860
Specificity
Detects human Oct-3/4 in Western blots and detects mouse Oct-3/4 in flow cytometry.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
POU5F1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB1759 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Oct-3/4 Antibody (240408) [Unconjugated]

  • 3-Oct
  • Oct-3/4
  • Oct4
  • Otf3g
  • Otf4
  • Pou5f1

Background

Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells.(2, 3) Oct-3/4 is required to sustain stem cell self-renewal and pluripotency.(4) It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells.

  1. Takeda, J. et al. (1992) Nucleic Acids Res. 20:4613.
  2. Scholer, H.R. et al. (1989) EMBO J. 8:2543.
  3. Rosner, M.H. et al. (1990) Nature 345:686.
  4. Niwa, H. et al. (2000) Nat. Genet. 24:372.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Oct-3/4 Antibody (MAB1759)(37)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: Bioassay, Flow Cytometry, ICC, ICC/IF, IHC-Fr, Western Blot.


Filter By Application
Bioassay
(1)
Flow Cytometry
(6)
ICC
(21)
ICC/IF
(1)
IHC-Fr
(1)
Western Blot
(4)
All Applications
Filter By Species
Human
(28)
Mouse
(6)
All Species
Showing Publications 1 - 10 of 37. Show All 37 Publications.
Publications using MAB1759 Applications Species
S Farahi, S Hosseini, H Ghanbarian, SM Hashemi, M Salehi, S Hosseini The Use of Trichostatin A during Pluripotent Stem Cell Generation Does Not Affect MHC Expression Level Stem Cells International, 2022;2022(0):9346767. 2022 [PMID: 35371264] (ICC/IF, Mouse) ICC/IF Mouse
K Kwa?niak, J Czarnik-Kw, K Malysheva, K Pogoda, O Korchynsky, P Rybojad, B Karczmarek, J Tabarkiewi LncCDH5-3:3 Regulates Apoptosis, Proliferation, and Aggressiveness in Human Lung Cancer Cells Cells, 2022;11(3):. 2022 [PMID: 35159188] (Western Blot, Human) Western Blot Human
SK Hota, KS Rao, AP Blair, A Khalilimey, KM Hu, R Thomas, K So, V Kameswaran, J Xu, BJ Polacco, RV Desai, N Chatterjee, A Hsu, JM Muncie, AM Blotnick, SAB Winchester, LS Weinberger, R Hüttenhain, IS Kathiriya, NJ Krogan, JJ Saucerman, BG Bruneau Brahma safeguards canalization of cardiac mesoderm differentiation Nature, 2022;602(7895):129-134. 2022 [PMID: 35082446] (ICC, Mouse) ICC Mouse
S Zhang, T Guo, Y Song, M Jiang, Y Jiang, T Dong, S Zhang, H Wang, X Hou Generation of a homozygous S100A1 knockout human embryonic stem cell line (WAe009-A-73) by the CRISPR/Cas9 editing system Stem Cell Research, 2021;59(0):102631. 2021 [PMID: 34953326] (Flow Cytometry, Human) Flow Cytometry Human
S Ma, Q Xu, R Bai, T Dong, Z Peng, X Liu Generation of a TPM1 homozygous knockout embryonic stem cell line by CRISPR/Cas9 editing Stem Cell Research, 2021;55(0):102470. 2021 [PMID: 34352617] (ICC, Human) ICC Human
X Liu, S Zhang, Y Chang, F Wu, R Bai Establishment of a KCNQ1 homozygous knockout human embryonic stem cell line by episomal vector-based CRISPR/Cas9 system Stem Cell Research, 2021;55(0):102467. 2021 [PMID: 34343827] (Flow Cytometry, Human) Flow Cytometry Human
W Wang, R Bai, X Song Generating dHAND homozygous knockout human embryonic stem cell line (WAe009-A-59) by episomal vector-based CRISPR/Cas9 system Stem Cell Research, 2021;55(0):102471. 2021 [PMID: 34332328] (Flow Cytometry, Human) Flow Cytometry Human
A Getachew, Z Yang, X Huang, F Wu, YY Liu, C Yan, F Yang, Y Li Generation of a TLR2 homozygous knockout human embryonic stem cell line WAe001-A-64 using CRISPR/Cas9 editing Stem Cell Research, 2021;54(0):102401. 2021 [PMID: 34058685] (Flow Cytometry, Human) Flow Cytometry Human
T Zhao, R Bai, F Wu, WJ Lu, J Zhang Generation of a TBX5 homozygous knockout embryonic stem cell line (WAe009-A-45) by CRISPR/Cas9 genome editing Stem Cell Research, 2021;51(0):102156. 2021 [PMID: 33497883] (Flow Cytometry, Human) Flow Cytometry Human
L Andreasson, H Evenbratt, R Mobini, S Simonsson Differentiation of induced pluripotent stem cells into definitive endoderm on Activin A-functionalized gradient surfaces J Biotechnol, 2020;0(0):. 2020 [PMID: 33147515] (Bioassay, Human) Bioassay Human
Show All 37 Publications.

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Bioinformatics

Gene Symbol POU5F1
Uniprot