Oct-3/4 Antibody (240408) [Unconjugated]

Western blot shows lysates of BG01V human embryonic stem cells and NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Oct‑3/4 Monoclonal Antibody (Catalog # MAB1759) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Oct-3/4 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Oct‑3/4 was detected in immersion fixed BG01V human embryonic stem cells using Rat Anti-Human/Mouse Oct‑3/4 Monoclonal Antibody (Catalog # MAB1759) at 10 µg/mL for 3 hours at room temperature. Cells were stained using Rhodamine Red-coupled anti-rat IgG (red) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu
• Applications: WB, CyTOF-ready, Flow, ICC/IF
• Clone: 240408
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Oct-3/4 Antibody (240408) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Oct‑3/4
  Met1-Asn265 (Met262Leu)
  Accession # Q01860
• Specificity: Detects human Oct-3/4 in Western blots and detects mouse Oct-3/4 in flow cytometry.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: POU5F1

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Oct-3/4 Antibody (240408) [Unconjugated]

• 3-Oct
• Oct-3/4
• Oct4
• Otf3g
• Otf4
• Pou5f1

Background

Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells.(2, 3) Oct-3/4 is required to sustain stem cell self-renewal and pluripotency.(4) It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells.

1. Takeda, J. et al. (1992) Nucleic Acids Res. 20:4613.
2. Scholer, H.R. et al. (1989) EMBO J. 8:2543.
3. Rosner, M.H. et al. (1990) Nature 345:686.
4. Niwa, H. et al. (2000) Nat. Genet. 24:372.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Oct-3/4 Antibody (MAB1759)(18)

Publications using MAB1759

• G Xu, D Guo, F Wu, N Abbas, K Lai, F Yuan, K You, Y Liu, Y Zhuang, Y Wu, Y Xu, Y Chen, F Yang, T Pan, YX Li Generation of a GDE heterozygous mutation human embryonic stem cell line WAe001-A-14 by CRISPR/Cas9 editing Stem Cell Res, 2017;27(0):38-41. 2017 [PMID: 29310060] (ICC, Human)
• F Wu, G Gao, T Pan, Z Yang, G Xu, N Abbas, Y Liu, Y Chen, S Tan, K You, X Ke, Y Zhuang, X Lin, F Yang, YX Li Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing Stem Cell Res, 2017;27(0):5-9. 2017 [PMID: 29278761]
• Y Xu, Y Wu, D Guo, G Gao, K Lai, F Yang, K Wang, H Wu, L Lai, J Li, K Xu, YX Li Generation of an ASGR1 homozygous mutant human embryonic stem cell line WAe001-A-6 using CRISPR/Cas9 Stem Cell Res, 2017;22(0):29-32. 2017 [PMID: 28952928] (ICC, Human)
• G Kaufman, D Skrtic Spatial development of gingival fibroblasts and dental pulp cells: Effect of extracellular matrix Tissue Cell, 2017;0(0):. 2017 [PMID: 28457531] (ICC, Mouse)
• P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia Oncotarget, 2017;8(17):28074-28092. 2017 [PMID: 28427209] (Western Blot, Human)
• P Wang, WW Wan, SL Xiong, H Feng, N Wu Cancer stem-like cells can be induced through dedifferentiation under hypoxic conditions in glioma, hepatoma and lung cancer Cell Death Discov, 2017;3(0):16105. 2017 [PMID: 28179999] (Western Blot, Human)
• Hagiwara, Kunie, Obayashi, Takeshi, Sakayori, Nobuyuki, Yamanishi, Emiko, Hayashi, Ryuhei, Osumi, Noriko, Nakazawa, Toru, Nishida, Kohji Molecular and cellular features of murine craniofacial and trunk neural crest cells as stem cell-like cells. PLoS ONE, 2014;9(1):e84072. 2014 [PMID: 24465393]
• Cortes-Dericks, Lourdes, Yazd, Ehsan Fa, Mowla, Seyed J, Schmid, Ralph A, Karoubi, Golnaz Suppression of OCT4B enhances sensitivity of lung adenocarcinoma A549 cells to cisplatin via increased apoptosis. Anticancer Res, 2013;33(12):5365-73. 2013 [PMID: 24324071] (Western Blot, Human)
• Pekkanen-Mattila M, Ojala M, Kerkela E, Rajala K, Skottman H, Aalto-Setala K The effect of human and mouse fibroblast feeder cells on cardiac differentiation of human pluripotent stem cells. Stem Cells Int, 2012;2012(0):875059. 2012 [PMID: 22315618] (ICC, Human)
• Kambal A, Mitchell G, Cary W, Gruenloh W, Jung Y, Kalomoiris S, Nacey C, McGee J, Lindsey M, Fury B, Bauer G, Nolta JA, Anderson JS Generation of HIV-1 resistant and functional macrophages from hematopoietic stem cell-derived induced pluripotent stem cells. Mol. Ther., 2011;19(3):584-93. 2011 [PMID: 21119622] (ICC, Human)
• Klim JR, Li L, Wrighton PJ A defined glycosaminoglycan-binding substratum for human pluripotent stem cells. Nat. Methods, 2010;7(12):989-94. 2010 [PMID: 21076418] (ICC, Human)
• Angel M, Yanik MF, Innate immune suppression enables frequent transfection with RNA encoding reprogramming proteins. PLoS ONE, 2010;5(7):e11756. 2010 [PMID: 20668695] (ICC, Human)
• Semenov O, Koestenbauer S, Riegel M, Zech N, Zimmermann R, Zisch A, Malek A Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation. Am J Obstet Gynecol, 0;202(2):193.e1-193.e1. 0 [PMID: 20035913]
• Ware CB, Wang L, Mecham BH, Shen L, Nelson AM, Bar M, Lamba DA, Dauphin DS, Buckingham B, Askari B, Lim R, Tewari M, Gartler SM, Issa JP, Pavlidis P, Duan Z, Blau CA Histone deacetylase inhibition elicits an evolutionarily conserved self-renewal program in embryonic stem cells. Cell Stem Cell, 2009;4(4):359-69. 2009 [PMID: 19341625] (ICC, Mouse)
• Hu T, Liu S, Breiter DR, Wang F, Tang Y, Sun S Octamer 4 small interfering RNA results in cancer stem cell-like cell apoptosis. Cancer Res., 2008;68(16):6533-40. 2008 [PMID: 18701476] (IHC-Fr, Mouse)
• Cordey M, Limacher M, Kobel S, Taylor V, Lutolf MP Enhancing the reliability and throughput of neurosphere culture on hydrogel microwell arrays. Stem Cells, 2008;26(10):2586-94. 2008 [PMID: 18669905] (ICC, Mouse)
• Hohenstein KA, Pyle AD, Chern JY, Lock LF, Donovan PJ Nucleofection mediates high-efficiency stable gene knockdown and transgene expression in human embryonic stem cells. Stem Cells, 2008;26(6):1436-43. 2008 [PMID: 18323409] (ICC, Human)
• Rajesh</LastName><ForeNam D</Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells. Stem Cells, 2007;25(2):490-9. 2007 [PMID: 17284653] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: POU5F1
• Uniprot:
  • Q01860()