OBFC1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: OBFC1 Antibody [NBP1-88743] - Staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli. Antibody staining is shown in green.

• Reactivity: Hu
• Applications: ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

OBFC1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids:PVFLAFAKLYIRDILDMKESRQVPGVFLYNGHPIKQVDVLGTVIGVRERDAFYSYGVDDSTGVINCICWKKLNTESVSAAPSAARELSLTSQLKKLQETIEQKTKI
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: STN1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
• Application Notes: Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  OBFC1 Protein (NBP1-88743PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for OBFC1 Antibody - BSA Free

• AAF44
• AAF-44
• alpha accessory factor 44
• bA541N10.2
• CST complex subunit STN1
• oligonucleotide/oligosaccharide-binding fold containing 1
• Oligonucleotide/oligosaccharide-binding fold-containing protein 1
• replication protein A 32 kDa subunit
• RP11-541N10.2
• RPA-32
• STN1FLJ22559
• Suppressor of cdc thirteen homolog

Background

OBFC1 and C17ORF68 (MIM 613129) are subunits of an alpha accessory factor (AAF) that stimulates the activity of DNApolymerase-alpha-primase (see MIM 176636), the enzyme that initiates DNA replication (Casteel et al., 2009 (PubMed19119139)). OBFC1 also appears to function in a telomere-associated complex with C17ORF68 and TEN1 (C17ORF106; MIM613130) (Miyake et al., 2009 (PubMed 19854130)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STN1