OATP1B3/SLCO1B3/OATP8 Overexpression Lysate

Images

 
Western Blot: OATP8 Overexpression Lysate (Adult Normal) [NBL1-16204] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OATP8.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

OATP1B3/SLCO1B3/OATP8 Overexpression Lysate Summary

Description

OATP1B3/SLCO1B3/OATP8 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC222317

Accession#: NM_019844

Protein Tag: C-MYC/DDK
Gene
SLCO1B3

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
77.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBL1-16204.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for OATP1B3/SLCO1B3/OATP8 Overexpression Lysate

  • Liver-specific organic anion transporter 2
  • liver-specific organic anion transporter 3TM13
  • LST2
  • LST-2
  • LST3
  • OATP1B3
  • OATP1B3LST-3TM13
  • OATP8
  • OATP-8
  • Organic anion transporter 8
  • organic anion transporter LST-3c
  • Organic anion-transporting polypeptide 8
  • SLC21A8
  • SLCO1B3
  • solute carrier family 21 (organic anion transporter), member 8
  • Solute carrier family 21 member 8
  • solute carrier organic anion transporter family member 1B3
  • solute carrier organic anion transporter family, member 1B3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for OATP1B3/SLCO1B3/OATP8 Lysate (NBL1-16204)(1)

We have publications tested in 1 application: WB.


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Video Protocols

WB Video Protocol

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OATP8 - A membrane transport protein responsible for cancer drug uptake
Human hepatocytes express important transport proteins that are responsible for the uptake and removal of organic anions from the blood. These proteins are members of the organic anion-transporting polypeptide (OATP) family and are essential for pr...  Read full blog post.

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Bioinformatics

Gene Symbol SLCO1B3