OATP1B3/SLCO1B3/OATP8 Overexpression Lysate

Western Blot: OATP8 Overexpression Lysate (Adult Normal) [NBL1-16204] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OATP8.

• Reactivity: Hu
• Applications: WB

OATP1B3/SLCO1B3/OATP8 Overexpression Lysate Summary

• Description:

  OATP1B3/SLCO1B3/OATP8 Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC222317
  
  Accession#: NM_019844
  
  Protein Tag: C-MYC/DDK
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
• Gene: SLCO1B3

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
  
  Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
• Theoretical MW: 77.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for OATP1B3/SLCO1B3/OATP8 Overexpression Lysate

• Liver-specific organic anion transporter 2
• liver-specific organic anion transporter 3TM13
• LST2
• LST-2
• LST3
• OATP1B3
• OATP1B3LST-3TM13
• OATP8
• OATP-8
• Organic anion transporter 8
• organic anion transporter LST-3c
• Organic anion-transporting polypeptide 8
• SLC21A8
• SLCO1B3
• solute carrier family 21 (organic anion transporter), member 8
• Solute carrier family 21 member 8
• solute carrier organic anion transporter family member 1B3
• solute carrier organic anion transporter family, member 1B3

Background

The OATPs (organic anion transporter polypeptides) are a family of transport proteins that have been found to mediate the uptake of organic ions into hepatocyte cells. These proteins fall into the solute carrier family 21A (SLC21A), and are expressed in liver, kidney, and brain. These polypeptides are involved in the excretion of potentially toxic compounds and therefore play a role in the overall detoxification system of the body. Unlike other members of the OATP family, OATP2 and 8 have been detected exclusively in the liver, and there is an 80% amino acid sequence homology between these polypeptides. Other names for OATP2 include SLC21A6, OATP-C, and LST-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for OATP1B3/SLCO1B3/OATP8 Lysate (NBL1-16204)(1)

Publication using NBL1-16204

• Thomson MM, Hines RN, Schuetz EG, Meibohm B. Expression Patterns of OATP 1B1 and OATP 1B3 Protein in Human Pediatric Liver. Drug Metab. Dispos. 2016-04-20 [PMID: 27098745] (WB)

Bioinformatics

• Gene Symbol: SLCO1B3