This product is intended for use as a positive control in Western Blot.
You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
77.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for OATP1B3/SLCO1B3/OATP8 Overexpression Lysate
Liver-specific organic anion transporter 2
liver-specific organic anion transporter 3TM13
Organic anion transporter 8
organic anion transporter LST-3c
Organic anion-transporting polypeptide 8
solute carrier family 21 (organic anion transporter), member 8
Solute carrier family 21 member 8
solute carrier organic anion transporter family member 1B3
solute carrier organic anion transporter family, member 1B3
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
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