OATP1B3/SLCO1B3/OATP8 Antibody (1E6.2F9)

Immunohistochemistry-Paraffin: OATP1B3/SLCO1B3/OATP8 Antibody (1E6.2F9) [NBP2-36492] - Analysis of formalin-fixed paraffin-embedded tissue section of human renal cell carcinoma using mouse monoclonal OATP1B3/SLCO1B3/OATP8 antibody (1E6.2F9) at 5 ug/ml concentration.

Flow Cytometry: OATP1B3/SLCO1B3/OATP8 Antibody (1E6.2F9) [NBP2-36492] - Validation of OATP1B3/SLCO1B3/OATP8 antibody (clone 1E6.2F9) on Jurkat cells with IgG3 kappa antibody as an isotype control. Primary antibody was used at 2.5ug/ million cells while the goat anti-mouse IgG-PE secondary antibody was employed at 0.5ug/million cells concentration.

Immunohistochemistry-Paraffin: OATP1B3/SLCO1B3/OATP8 Antibody (1E6.2F9) [NBP2-36492] - Analysis of formalin-fixed paraffin-embedded tissue section of normal human spleen using mouse monoclonal OATP1B3/SLCO1B3/OATP8 antibody (clone 1E6.2F9) at 5 ug/ml concentration.

Product Discontinued

OATP1B3/SLCO1B3/OATP8 Antibody (1E6.2F9) Summary

• Immunogen: Two synthetic peptides made to the human OATP8 protein sequence (between residues 648-700). [Swiss-Prot Q9NPD5]
• Localization: Basolateral cell membrane
• Predicted Species: Primate (98%). Backed by our 100% Guarantee.
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLCO1B3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2.5 ug/ million cells
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
• Control:

  OATP1B3/SLCO1B3/OATP8 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for OATP1B3/SLCO1B3/OATP8 Antibody (1E6.2F9)

• Liver-specific organic anion transporter 2
• liver-specific organic anion transporter 3TM13
• LST2
• LST-2
• LST3
• OATP1B3
• OATP1B3LST-3TM13
• OATP8
• OATP-8
• Organic anion transporter 8
• organic anion transporter LST-3c
• Organic anion-transporting polypeptide 8
• SLC21A8
• SLCO1B3
• solute carrier family 21 (organic anion transporter), member 8
• Solute carrier family 21 member 8
• solute carrier organic anion transporter family member 1B3
• solute carrier organic anion transporter family, member 1B3

Background

Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3, LST-1, and PGT), have been cloned and characterized from various tissues. The OATP family of proteins share significant sequence homology, and general secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus). Oatp2 (also called OATP-B1; 661 aa; 12 TM), cloned from rat brain, shares significant homology with rat oatp1 and OAT-K1 (77%) and human OATP (73%). It is highly expressed in brain, liver, and kidney but not in heart, spleen, lung, muscle, and testes. OATP2 mediates uptake of taurocholate, estrogen conjugates, and cardiac glycosides ouabain. Unlike other OATPS, OATP2 also transport digioxin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLCO1B3
• Entrez:
  • 28234(Human)
• OMIM:
  • 237450(Human)
• Uniprot:
  • Q9NPD5(Human)