O-GlcNAcase/OGA/MGEA5 Antibody (JG40-05)

Western Blot: O-GlcNAcase/OGA/MGEA5 Antibody (JG40-05) [NBP2-76848] - Western blot analysis of MGEA5 on PC-3M and SH-SY-5Y cell lysates using anti-MGEA5 antibody at 1/500 dilution.

Immunohistochemistry-Paraffin: O-GlcNAcase/OGA/MGEA5 Antibody (JG40-05) [NBP2-76848] - Analysis of mouse testis tissue using anti-MGEA5 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: O-GlcNAcase/OGA/MGEA5 Antibody (JG40-05) [NBP2-76848] - Analysis of human tonsil tissue using anti-MGEA5 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: O-GlcNAcase/OGA/MGEA5 Antibody (JG40-05) [NBP2-76848] - Analysis of human pancreas tissue using anti-MGEA5 antibody. Counter stained with hematoxylin.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clone: JG40-05
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

O-GlcNAcase/OGA/MGEA5 Antibody (JG40-05) Summary

• Description: Novus Biologicals Rabbit O-GlcNAcase/OGA/MGEA5 Antibody (JG40-05) (NBP2-76848) is a recombinant monoclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human O-GlcNAcase/OGA/MGEA5 aa 2-103 / 916. (SwissProt: O60502 Human; SwissProt: Q9EQQ9 Mouse)
• Localization: Cytoplasm. Nucleus.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: OGA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 103 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for O-GlcNAcase/OGA/MGEA5 Antibody (JG40-05)

• bifunctional protein NCOAT
• FLJ11229
• GH84
• HEXC
• HEXC3
• Hexosaminidase B
• hyaluronidase in meningioma
• KIAA0679
• MEA5FLJ23355
• meningioma expressed antigen 5 (hyaluronidase)
• Meningioma-expressed antigen 5
• MGEA5
• NCOAT
• Nuclear cytoplasmic O-GlcNAcase and acetyltransferase
• OGA
• OGlcNAcase
• O-GlcNAcase

Background

The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OGA