Nucleostemin Antibody

Western Blot: Nucleostemin Antibody [NB100-1569] - Detection of mouse GNL3 by western blot. Samples:Whole cell lysate (50 ug) from TMCK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GNL3 antibody NB100-1569 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry: Nucleostemin Antibody [NB100-1569] - Detection of human GNL3 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-GNL3 (NB100-1569). Detection: DAB

Western Blot: Nucleostemin Antibody [NB100-1569] - Detection of human GNL3 by western blot. Samples:Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GNL3 antibody NB100-1569 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunohistochemistry-Paraffin: Nucleostemin Antibody [NB100-1569] - Human skin carcinoma. Antibody used at a dilution of 1:1,000.

Immunohistochemistry: Nucleostemin Antibody [NB100-1569] - Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-GNL3 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Immunohistochemistry: Nucleostemin Antibody [NB100-1569] - Detection of mouse GNL3 by immunohistochemistry. Sample: FFPE section of mouse CT26 colon carcinoma. Antibody: Affinity purified rabbit anti-GNL3 (NB100-1569). Detection: DAB

Immunoprecipitation: Nucleostemin Antibody [NB100-1569] - Detection of human GNL3 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GNL3 antibody NB100-1569(lot NB100-1569-3) used for IP at 6 ug per reaction. GNL3 was also immunoprecipitated by a previous lot of this antibody (lot NB100-1569-2). For blotting immunoprecipitated GNL3, NB100-1569 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Nucleostemin Antibody Summary

• Description: Novus Biologicals Rabbit Nucleostemin Antibody (NB100-1569) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 500 and the C-terminus (residue 549) of human Guanine Nucleotide Binding Protein-Like 3 (Nucleostemin) using the numbering given in entry NP_055181.3 (GeneID 26354).
• Marker: Embryonic Stem Cell Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GNL3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1-4 ug/mg of lysate
  • Western Blot 1:2000-1:10000
• Theoretical MW: 62 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Nucleostemin Antibody

• C77032
• E2IG3NNP47
• estradiol-induced nucleotide binding protein
• GNL3
• guanine nucleotide binding protein-like 3 (nucleolar)
• guanine nucleotide-binding protein-like 3
• MGC800
• Novel nucleolar protein 47
• NSE2-induced gene 3 protein
• Nucleolar GTP-binding protein 3
• Nucleostemin

Background

Nucleostemin is a protein found in the nucleoli of embryonic stem cells, adult CNS stem cells, primitive cells in the bone marrow and cancer cells. It is not in the differentiated cells of most adult tissues. It has been suggested to play a role in controlling the cell-cycle progression in stem cells and cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GNL3
• Entrez:
  • 26354(Human)
  • 30877(Mouse)
• Uniprot:
  • Q9BVP2(Human)