![Western Blot: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-PPT1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize2/PPT1-Antibody-(1F10)-Western-Blot-NBP2-45388-img0007.jpg "Western Blot: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-PPT1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC, IHC-P |
| Clone | OTI1F10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein fragment corresponding to amino acids 100-306 of human PPT1 (NP_000301) produced in E.coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PPT1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 31.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Diseases for PPT1 Antibody (NBP2-45388)Discover more about diseases related to PPT1 Antibody (NBP2-45388).
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PTMs for PPT1 Antibody (NBP2-45388)Learn more about PTMs related to PPT1 Antibody (NBP2-45388).
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| Gene Symbol | PPT1 |
![Immunohistochemistry-Paraffin: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/PPT1-Antibody-(1F10)-Immunohistochemistry-Paraffin-NBP2-45388-img0012.jpg "Immunohistochemistry-Paraffin: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Western Blot: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPT1.](http://images.novusbio.com/fullsize2/PPT1-Antibody-(1F10)-Western-Blot-NBP2-45388-img0006.jpg "Western Blot: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPT1.")
![Immunohistochemistry-Paraffin: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/PPT1-Antibody-(1F10)-Immunohistochemistry-Paraffin-NBP2-45388-img0008.jpg "Immunohistochemistry-Paraffin: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry-Paraffin: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/PPT1-Antibody-(1F10)-Immunohistochemistry-Paraffin-NBP2-45388-img0009.jpg "Immunohistochemistry-Paraffin: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry-Paraffin: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/PPT1-Antibody-(1F10)-Immunohistochemistry-Paraffin-NBP2-45388-img0010.jpg "Immunohistochemistry-Paraffin: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Immunohistochemistry-Paraffin: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/PPT1-Antibody-(1F10)-Immunohistochemistry-Paraffin-NBP2-45388-img0011.jpg "Immunohistochemistry-Paraffin: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)")
![Western Blot: Tripeptidyl-Peptidase I/TPP1 Antibody [NBP1-31758] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize2/Tripeptidyl-Peptidase-I-TPP1-Antibody-Western-Blot-NBP1-31758-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Tripeptidyl-Peptidase I/TPP1 Antibody [NBP1-31758] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize2/Tripeptidyl-Peptidase-I-TPP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31758-img0005.jpg)
![Immunohistochemistry-Paraffin: Tripeptidyl-Peptidase I/TPP1 Antibody [NBP1-31758] - Paraffin-embedded mouse liver. TPP1 antibody [N1C1] diluted at 1:500.](http://images.novusbio.com/fullsize2/Tripeptidyl-Peptidase-I-TPP1-Antibody-Immunohistochemistry-Paraffin-NBP1-31758-img0009.jpg)
![Western Blot: CLN3 Antibody [NBP2-43782] - Analysis of A. 30 ug NT2D1 whole cell lysate/extract 10 % SDS-PAGE CLN3 antibody dilution: 1:1000](http://images.novusbio.com/fullsize2/CLN3-Antibody-Western-Blot-NBP2-43782-img0002.jpg)
![Western Blot: CLN3 Antibody [NBP2-43782] - Analysis of A. 30 ug Jurkat whole cell lysate/extract B. 30 ug Raji whole cell lysate/extract C. 30 ug NCI-H929 whole cell lysate/extract 10 % SDS-PAGE CLN3 antibody dilution: 1:1000.](http://images.novusbio.com/fullsize2/CLN3-Antibody-Western-Blot-NBP2-43782-img0001.jpg)
![Western Blot: ER beta/NR3A2 Antibody (14C8) [NB200-305] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with diluted at 1:5000.](http://images.novusbio.com/fullsize2/ER-beta-NR3A2-Antibody-(14C8)-Western-Blot-NB200-305-img0006.jpg)
![Immunohistochemistry-Paraffin: Estrogen Receptor beta Antibody (14C8) [NB200-305] - Infiltrating lobular carcinoma of the breast.](http://images.novusbio.com/fullsize2/Estrogen Receptor beta Antibody (14C8)-Immunohistochemistry-Paraffin-NB200-305-img0003.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560] - Analysis of recombinant ER alpha.](http://images.novusbio.com/fullsize2/ER-alpha-NR3A1-Antibody-(33)-Western-Blot-NB300-560-img0004.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast tissue (B) and magnified section (C) compared with an isotype control (A).](http://images.novusbio.com/fullsize2/ER-alpha-NR3A1-Antibody-(33)-Immunohistochemistry-Paraffin-NB300-560-img0010.jpg)
![Immunohistochemistry-Frozen: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast carcinoma (B) and magnified section (C) compared with an isotype control (A).](http://images.novusbio.com/fullsize2/ER-alpha-NR3A1-Antibody-(33)-Immunohistochemistry-Frozen-NB300-560-img0009.jpg)
![Western Blot: CSP Antibody [H00080331-B01P] - Analysis of DNAJC5 expression in rat brain.](http://images.novusbio.com/fullsize2/CSP-Antibody-Western-Blot-H00080331-B01P-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CSP Antibody [H00080331-B01P] - Analysis of purified antibody to DNAJC5 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize2/CSP-Antibody-Immunocytochemistry-Immunofluorescence-H00080331-B01P-img0001.jpg)
![Western Blot: CSP Antibody [H00080331-B01P] - Analysis of DNAJC5 expression in transfected 293T cell line by DNAJC5 polyclonal antibody. Lane 1: DNAJC5 transfected lysate(21.78 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize2/CSP Antibody-Western Blot-H00080331-B01P-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Substance P Antibody [NB300-187] - Staining of trigeminal ganglia from P3-P5 rat pups. BMC Neuroscience 2010, 11:32doi:10.1186/1471-2202-11-32](http://images.novusbio.com/fullsize2/Substance-P-Antibody-Immunocytochemistry-Immunofluorescence-NB300-187-img0005.jpg)
![Immunohistochemistry: Substance P Antibody [NB300-187] - Spinal Cord Dorsal Horn (Human).](http://images.novusbio.com/fullsize2/Substance P Antibody-Immunohistochemistry-NB300-187-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Substance P Antibody [NB300-187] - Detection of Substance P in rat spinal cord dorsal horn (red fluorescence). DAPI (blue) was used as counter stain.](http://images.novusbio.com/fullsize2/Substance P Antibody-Immunocytochemistry-Immunofluorescence-NB300-187-img0003.jpg)
![Western Blot: Nucleolin Antibody [NB600-241] - Detection of nucleolin in crude PD31 nuclear extracts.](http://images.novusbio.com/fullsize2/Nucleolin-Antibody-Western-Blot-NB600-241-img0005.jpg)
![Immunocytochemistry: Nucleolin Antibody [NB600-241] - Human prostate epithelial cells stained with anti-nucleolin (red), CFSE (green) retaining cell has increased nucleolin in nucleolus. DAPI (blue). This image was submitted via customer Review.](http://images.novusbio.com/fullsize2/Nucleolin Antibody-Immunocytochemistry-NB600-241-img0011.jpg)
![Immunohistochemistry: Nucleolin Antibody [NB600-241] - Human prostate epithelial cells stained with anti-nucleolin (red), CFSE (green) retaining cell has increased nucleolin in nucleolus. DAPI (blue). Verified customer review.](http://images.novusbio.com/fullsize2/Nucleolin-Antibody-Immunohistochemistry-NB600-241-img0010.jpg)
![Western Blot: CLN5 Antibody [NBP1-94150] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize2/CLN5-Antibody-Western-Blot-NBP1-94150-img0007.jpg)
![Immunohistochemistry-Paraffin: CLN5 Antibody [NBP1-94150] - Staining of human gallbladder shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/CLN5-Antibody-Immunohistochemistry-Paraffin-NBP1-94150-img0008.jpg)
![Western Blot: DBT Antibody [NBP1-89522] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize2/DBT-Antibody-Western-Blot-NBP1-89522-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: DBT Antibody [NBP1-89522] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.](http://images.novusbio.com/fullsize2/DBT-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89522-img0007.jpg)
![Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining in human kidney and pancreas tissues using anti-DBT antibody. Corresponding DBT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-89522-img0010.jpg)
![Western Blot: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Rat brain lysate showing specific immunolabeling of the ~70k ChAT.](http://images.novusbio.com/fullsize2/Choline-Acetyltransferase-ChAT-Antibody-Western-Blot-NBP1-30052-img0002.jpg)
![Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP1-30052] - Rat brainstem showing specific labeling using the anti-ChAT antibody. Image courtesy of Dr. Robert Sloviter, University of Arizona.](http://images.novusbio.com/fullsize2/Choline-Acetyltransferase-ChAT-Antibody-Immunohistochemistry-Paraffin-NBP1-30052-img0003.jpg)
![Western Blot: CLN8 Antibody [NBP1-91333] - Titration: 0.2-1 ug/ml, Positive Control: Hela cell lysate.](http://images.novusbio.com/fullsize2/CLN8 Antibody-Western Blot-NBP1-91333-img0003.jpg)
![Western Blot: CLN6 Antibody [NBP2-39066] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue](http://images.novusbio.com/fullsize2/CLN6 Antibody-Western Blot-NBP2-39066-img0002.jpg)
![Immunohistochemistry-Paraffin: CLN6 Antibody [NBP2-39066] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/CLN6-Antibody-Immunohistochemistry-Paraffin-NBP2-39066-img0003.jpg)
![Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)](http://images.novusbio.com/fullsize2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-Paraffin-NBP2-34260-img0002.jpg)
![SDS-Page: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260]](http://images.novusbio.com/fullsize2/Insulin Antibody (E2-E3 (same as INS04))-SDS-Page-NBP2-34260-img0003.jpg)
![Western Blot: Glutamine Synthetase Antibody [NB110-41404] - Analysis of glutamine synthase. 40ug of lysates from mouse (Lanes M), rat (Lanes R), pig (Lanes P), bovine (Lanes B), or human (Lanes Hu) retina were probed. A 42 kDa band was identified in lysates from retinas of all species.](http://images.novusbio.com/fullsize2/Glutamine Synthetase Antibody-Western Blot-NB110-41404-img0003.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody [NB110-41404] - Localization of glutamine synthase in the retina. Paraffin sections of mouse (A, B), rat (C, D, G-I), or human (E, F) retina fixed in 4% paraformaldehyde were reacted with anti-glutamine synthase (red fluorescence staining in B, D, G, I, and brown immuno-peroxidase reaction [using ABC kit and visualization with DAB product in F]. Nuclei in some immunofluorescence experiments (A-D) were stained with DAPI (shown in cyan), and with nuclear fast red in E and F. On inspection at low magnification, anti- glutamine synthase reacted with a single population of cells extending from the ganglion cell layer (GCL) through the inner nuclear layer (INL). No signal was detected in controls either pre-incubated with 100ug/ml of the immunizing peptide (A) or with pre-immune serum (C, E). The pattern of staining observed in all experiments is typicals. This finding was confirmed by co-localization (indicated by yellow in I) of glutamine synthase (red in G) with antoher marker of glutamine synthase (green in H).](http://images.novusbio.com/fullsize2/Glutamine Synthetase Antibody-Immunohistochemistry-NB110-41404-img0002.jpg)
![Immunohistochemistry-Paraffin: Met-enkephalin Antibody [NBP1-90944] - Staining in human adrenal gland and kidney tissues using anti-PENK antibody. Corresponding PENK RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/Met-enkephalin-Antibody-Immunohistochemistry-Paraffin-NBP1-90944-img0010.jpg)
![Immunohistochemistry-Paraffin: Met-enkephalin Antibody [NBP1-90944] - Staining of human adrenal gland shows strong cytoplasmic positivity in medullary cells.](http://images.novusbio.com/fullsize2/Met-enkephalin-Antibody-Immunohistochemistry-Paraffin-NBP1-90944-img0008.jpg)
![Immunohistochemistry-Paraffin: Met-enkephalin Antibody [NBP1-90944] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize2/Met-enkephalin-Antibody-Immunohistochemistry-Paraffin-NBP1-90944-img0009.jpg)
![SDS-Page: Mouse IgG1 Isotype Control [NBP1-97005] - Lane 1:, Non-reduced. M: Opal Pre-stained Ladder. Lane 2:, Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](http://images.novusbio.com/images2/Mouse-IgG1-Isotype-Control-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control [NBP1-97005] - Lane 1:, Non-reduced. M: Opal Pre-stained Ladder. Lane 2:, Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")
