PPT1 Antibody (OTI1F10)

Western Blot: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-PPT1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry-Paraffin: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Western Blot: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPT1.

Immunohistochemistry-Paraffin: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry-Paraffin: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry-Paraffin: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry-Paraffin: PPT1 Antibody (1F10) [NBP2-45388] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

• Reactivity: Hu
• Applications: WB, IHC
• Clone: OTI1F10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

PPT1 Antibody (OTI1F10) Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 100-306 of human PPT1 (NP_000301) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500
  • Immunohistochemistry-Paraffin 1:500
  • Western Blot 1:2000
• Theoretical MW: 31.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PPT1 Antibody (OTI1F10)

• ceroid-palmitoyl-palmitoyl-protein thioesterase 1
• CLN1
• INCL
• Palmitoyl-protein hydrolase 1
• palmitoyl-protein thioesterase 1
• PPT
• PPT1
• PPT-1

Background

Palmitoyl-protein thioesterase (PPT) is a small glycoprotein that removes palmitate groups from cysteine residues in lipid-modified proteins. PPT is thought to be involved in the catabolism of lipid-modified proteins (Camp et al., 1994 [PubMed 7916016]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PPT1 Antibody (NBP2-45388)(2)

Publications using NBP2-45388

• Peviani M, Das S, Patel J et al. An innovative hematopoietic stem cell gene therapy approach benefits CLN1 disease in the mouse model EMBO molecular medicine 2023-04-11 [PMID: 36876653] (Western Blot, Human)
  
  Details:
  Dilution 1:500
• Tiu AC, Yang J, Asico LD et al. Lipid rafts are required for effective renal D1 dopamine receptor function FASEB J. 2020-04-07 [PMID: 32259353] (WB, Human)

Bioinformatics

• Gene Symbol: PPT1