NTB-A/SLAMF6/CD352 Antibody (13G3-19D) - BSA Free

Flow Cytometry: NTB-A/SLAMF6/CD352 Antibody (13G3-19D) [NBP1-43344] - Staining of BALB/c splenocytes with 0.25 ug of Mouse IgG2a kappa Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse Ly-108 Purified (filled histogram) followed by Anti-Mouse IgG2a Biotin and Streptavidin PE. Cells in the lymphocyte gate were used for analysis.

• Reactivity: Mu
• Applications: WB, Flow, IP
• Clone: 13G3-19D
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

NTB-A/SLAMF6/CD352 Antibody (13G3-19D) - BSA Free Summary

• Immunogen: The immunogen for this antibody was Ly108.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLAMF6
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: This 13G3-19D antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS (pH 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for NTB-A/SLAMF6/CD352 Antibody (13G3-19D) - BSA Free

• Activating NK receptor
• CD352 antigen
• CD352
• KALIFLJ50657
• Ly108
• NK-T-B-antigen
• NTBA receptor
• NTBA
• NTB-A
• NTB-AMGC104953
• NTBAT- and B-cell antigen
• SF2000
• SLAM family member 6
• SLAMF6

Background

The 13G3-19D monoclonal antibody reacts with mouse Ly108, the mouse homologue of human SLAMF6 (signaling lymphocyte activation molecule family member 6). Mouse Ly108 is also known as NTB-A (NK cell, T cell, B cell antigen), and is expressed on NK, T cells, and B cells. Engagement of the Ly108 receptor enhances NK cell activity and expression of IFN-gamma, and contributes to the inability of NK cells to kill Epstein Barr virus-infected B cells in X-linked lymphoproliferative disease. CD4+ T cells from Ly108 knock-out mice exhibit diminished ability to produce IL-4 and defective neutrophil functions. Staining has not revealed any strain differences between C57BL/6 and BALB/c mice. Other strains have not been tested.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLAMF6