SLAM/CD150 Antibody

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Mouse splenocytes were treated for 2 days with 50 ng/mL PMA and 500 ng/mL Ca2+ ionomycin then stained with Sheep Anti-Mouse SLAM/CD150 Antigen Affinity‑purified Poly­clonal Antibody (Catalog # AF4330, filled ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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SLAM/CD150 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SLAM/CD150
Thr25-Pro242
Accession # Q9QUM4
Specificity
Detects mouse SLAM/CD150 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human SLAM is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
SLAMF1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF4330 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SLAM/CD150 Antibody

  • CD 150
  • CD150
  • CD150IPO-3
  • CDw150
  • IPO-3
  • signaling lymphocytic activation molecule family member 1
  • signaling lymphocytic activation molecule
  • SLAM
  • SLAMCD150 antigen
  • SLAMF1

Background

The type I transmembrane glycoprotein Signaling Lymphocytic Activation Molecule (SLAM), also known as CD150, is the prototypic member of the SLAM subgroup of the CD2 protein family. CD2 family proteins function as adhesion molecules and modulators of the immune response (1). Mouse SLAM consists of a 218 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 23 aa transmembrane segment, and a 78 aa cytoplasmic domain with three immunoreceptor tyrosine switch motifs (ITSM) (2). Alternate splicing generates an isoform with a substituted cytoplasmic domain (2). Within the ECD, mouse SLAM shares 58% and 83% aa sequence identity with human and rat SLAM, respectively. It is expressed as a 75 kDa molecule of which approximately 30 kDa is N-linked carbohydrate (2). SLAM is expressed on T cells, B cells, thymocytes, macrophages, dendritic cells, platelets, and hematopoietic stem cells (2‑7). It is upregulated on activated B cells and CD4+ and CD8+ T cells, although it is downregulated on Th2 polarized cells (2, 3, 8). SLAM interacts homophilically with low affinity, and this interaction induces a Th0/Th1 response characterized by clonal expansion, production of IFN-gamma , and increased cytolytic activity of CD8+ T cells (2, 3, 9‑11). SLAM ligation also promotes B cell activation, allergen-induced eosinophil and mast cell activation, and macrophage responsiveness to LPS (4, 8, 12). In humans, SLAM functions as a cellular entry receptor for measles virus (13, 14).

  1. Ma, C.S. et al. (2007) Annu. Rev. Immunol. 25:337.
  2. Castro, A.G. et al. (1999) J. Immunol. 163:5860.
  3. Cocks, B.G. et al. (1995) Nature 376:260. 
  4. Wang, N. et al. (2004) J. Exp. Med. 199:1255. 
  5. Hahm, B. et al. (2004) Virology 323:292. 
  6. Nanda, N. et al. (2005) Blood 106:3028. 
  7. Kiel, M.J. et al. (2005) Cell 121:1109. 
  8. Punnonen, J. et al. (1997) J. Exp. Med. 185:993.
  9. Mavaddat, N. et al. (2000) J. Biol. Chem. 275:28100.
  10. Aversa, G. et al. (1997) J. Immunol. 158:4036.
  11. Mehrle, S. et al. (2008) Mol. Immunol. 45:796.
  12. Wang, N. et al. (2006) Am. J. Respir. Cell Mol. Biol. 35:206.
  13. Tatsuo, H. et al. (2000) Nature 406:893.
  14. Hsu, E.C. et al. (2001) Virology 279:9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for SLAM/CD150 Antibody (AF4330) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

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 SLAM/CD150 AF4330
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Mouse 10/21/2018
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Summary

Sample TestedBone marrow
SpeciesMouse
LotI don't have it any more

Comments

CommentsWhole Femur Section, Fixed-frozen and decalcified

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Verified Customer
10/21/2018
Application:
Species: Mouse

Bioinformatics

Gene Symbol SLAMF1
Uniprot