2B4/CD244/SLAMF4 Antibody [Unconjugated]

Images

 
2B4/CD244/SLAMF4 was detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human 2B4/CD244/SLAMF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1039) at 1 µg/mL ...read more
Western blot shows lysates of NK‑RL human natural killer lymphocytic leukemia cell line, NK‑92 human natural killer lymphoma cell line, and Daudi human Burkitt's lymphoma cell line (negative control). PVDF ...read more
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human NCAM‑1/CD56 APC‑conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Goat Anti-Human 2B4/CD244/SLAMF4 Antigen ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready, ELISA(Cap), Func
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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2B4/CD244/SLAMF4 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human 2B4/CD244/SLAMF4
Cys22-Arg221
Accession # NP_057466
Specificity
Detects human 2B4/CD244/SLAMF4 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant mouse 2B4, recombinant human (rh) SLAM, rhCD48, and rhCD84 is observed.
Details of Functionality
ActivityAssaywCitation not found None None None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CD244
Endotoxin Note
<0.01 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Agonist Activity
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Human 2B4/CD244/SLAMF4 Antibody (Catalog # AF1039)
Standard: Recombinant Human 2B4/CD244/SLAMF4 Fc Chimera (Catalog # 1039-2B)
Publications
Read Publications using
AF1039 in the following applications:

  • ICC
    1 publication
  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for 2B4/CD244/SLAMF4 Antibody [Unconjugated]

  • 2B4
  • CD244 antigen
  • CD244 molecule, natural killer cell receptor 2B4
  • CD244
  • h2B4
  • NAIL
  • NAILNK cell activation-inducing ligand
  • natural killer cell receptor 2B4
  • NKR2B4
  • NKR2B4NK cell type I receptor protein 2B4,2B4CD244 natural killer cell receptor 2B4
  • Nmrk
  • SLAMF4
  • SLAMF4NK cell activation inducing ligand NAIL

Background

2B4, also known as CD244 and SLAMF4, is a 66 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 protein family. SLAM family proteins have an extracellular domain (ECD) with two or four Ig-like domains and at least two cytoplasmic immunoreceptor tyrosine-based switch motifs (ITSMs). 2B4 interacts with CD48, while other SLAM family proteins interact homophilically (1‑4). Mature human 2B4 consists of a 208 amino acid (aa) ECD with two Ig-like domains, a 21 aa transmembrane segment, and a 120 aa cytoplasmic domain with four ITSMs (5, 6). Three additional splice variants of human 2B4 have deletions of the short region between the Ig-like domains, the second Ig-like domain, or a portion of the cytoplasmic tail. Within the ECD, human 2B4 shares 46% and 40% aa sequence identity with mouse and rat 2B4, respectively. The ECD of human 2B4 shares 17%‑24% aa sequence identity with comparable regions of human CD2 family members BLAME, CD2F-10, CD84, CD229, CRACC, NTB-A, and SLAM. 2B4 is expressed on all NK cells, gamma δ T cells, monocytes, some CD4+ and CD8+ T cells, and some dendritic cells (7). CD48 mediates 2B4+ cell interactions with nearly all hematopoietic cell types, including cells of the same type (8‑10). 2B4/CD48 signaling cooperates with other receptor systems to either promote or inhibit NK and CD8+ T cell activation (7‑13). The inhibitory activities are distinct from those of MHC I restricted inhibitory NK cell receptors (12, 13). Ligation of 2B4 with antibodies or CD48 constructs can either directly trigger inhibitory signaling or disrupt an inhibitory interaction, leading to cellular activation (9, 12). The inhibitory effect is associated with the long form of 2B4, while the activation is associated with the short form (9, 14). 2B4 can also induce signaling through CD48 (10, 15).
  1. Bhat, R. et al. (2006) J. Leukoc. Biol. 79:417.
  2. Veillette, A. (2006) Nat. Rev. Immunol. 6:56.
  3. McNerney, M.E. et al. (2005) Mol. Immunol. 42:489.
  4. Assarsson, E. et al. (2005) J. Immunol. 175:2045.
  5. Boles, K.S. et al. (1999) Tissue Antigens 54:27.
  6. Kubin, M.Z. et al. (1999) Eur. J. Immunol. 29:3466.
  7. Nakajima, H. et al. (1999) Eur. J. Immunol. 29:1676.
  8. Lee, K.M. et al. (2006) Blood 107:3181.
  9. Mooney, J.M. et al. (2004) J. Immunol. 173:3953.
  10. Assarsson, E. et al. (2004) J. Immunol. 173:174.
  11. Bryceson, Y.T. et al. (2006) Blood 107:159.
  12. Lee, K-M. et al. (2004) J. Exp. Med. 199:1245.
  13. McNerney, M.E. et al. (2005) Blood 106:1337.
  14. Schatzle, J.D. et al. (1999) Proc. Natl. Acad. Sci. USA 96:3870.
  15. Messmer, B. et al. (2006) J. Immunol. 176:4646.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 2B4/CD244/SLAMF4 Antibody (AF1039)(2)

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Bioinformatics

Gene Symbol CD244
Entrez
Uniprot