CD48/SLAMF2 Antibody [Unconjugated]

CD48/SLAMF2 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse CD48/SLAMF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3327) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready, ELISA(Cap), ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CD48/SLAMF2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CD48/SLAMF2
  Phe23-Arg216
  Accession # BAE96326
• Specificity: Detects mouse CD48/SLAMF2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human CD48, recombinant mouse (rm) OX40, rm2B4, and rmNTB-A, and rmCD2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CD48
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse CD48/SLAMF2 Antibody (Catalog # AF3327)
  ELISA Detection:Mouse CD48/SLAMF2 Biotinylated Antibody (Catalog # BAF3327)
  Standard: Recombinant Mouse CD48/SLAMF2 (Catalog # 3327-CD)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD48/SLAMF2 Antibody [Unconjugated]

• BCM1 surface antigen
• BCM1
• BCM1Leukocyte antigen MEM-102
• BLAST
• BLAST1
• BLAST1TCT.1
• B-lymphocyte activation marker BLAST-1
• CD48 antigen (B-cell membrane protein)
• CD48 antigen
• CD48 molecule
• CD48
• hCD48
• mCD48
• MEM-102
• SLAMF2

Background

CD48, also known as BLAST-1, BCM-1, and SLAMF2, is a 65 kDa GPI-linked protein in the CD2 family of immunoglobulin superfamily proteins (1‑3). The mouse CD48 cDNA encodes a 240 amino acid (aa) precursor that includes a 22 aa signal sequence, a 195 aa mature protein that contains one Ig-like V-type domain and one Ig-like C2-type domain, and a 23 aa C-terminal propeptide (4). A soluble form of CD48 has been detected in the serum of lymphoid leukemia and arthritis patients (5). Mouse CD48 shares 51% and 68% aa sequence identity with human and rat CD48, respectively. It shares 18%‑26% aa sequence identity with comparable regions of mouse 2B4, BLAME, CD2F-10, CD84, CD229, CRACC, NTB-A, and SLAM. CD48 is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors (4, 6). Among dendritic cells (DC), CD48 is selectively expressed on circulating myeloid DC and resident bone marrow and thymus DC (7). CD2, 2B4, and heparan sulfate function as CD48 ligands (8 - 10). CD48 is competent to transduce signals and can also trigger signaling through CD2 or 2B4 (8, 11). CD48-CD2 interactions promote T cell activation and class switching to IgG_2a in B cells (8,12). High affinity CD48-2B4 interactions can either promote or inhibit NK cell and cytotoxic T cell (CTL) activation (7, 11, 13, 14). CD48-2B4 ligation does not directly trigger CTL activity but enhances signaling from the T cell receptor (13). CD48-2B4 mediated inhibition of NK cell activity is distinct from MHC I-restricted mechanisms (15). CD48 expressed on NK cells is coactivating, whereas CD48 expressed on other cell types inhibits NK cell activation (14). Both CD48 expressing and nonexpressing cells can be targets of NK cell or CTL-mediated lysis (13, 16).

1. Assarsson, E. et al. (2005) J. Immunol. 175:2045.
2. Bhat, R. et al. (2006) J. Leukoc. Biol. 79:417.
3. Loertscher, R. and P. Lavery (2002) Transpl. Immunol. 9:93.
4. Wong, Y.W. et al. (1990) J. Exp. Med. 171:2115.
5. Smith, G.M. et al. (1997) J. Clin. Immunol. 17:502.
6. Keil, M.J. et al. (2005) Cell 121:1109.
7. Morandi, B. et al. (2005) J. Immunol. 175:3690.
8. Kato, K. et al. (1992) J. Exp. Med. 176:1241.
9. Latchman, Y. et al. (1998) J. Immunol. 161:5809.
10. Ianelli, C.J. et al. (1998) J. Biol. Chem. 273:23367.
11. Messmer, B. et al. (2006) J. Immunol. 176:4646.
12. Gao, N. et al. (2005) J. Immunol. 174:4113.
13. Lee, K.-M. et al. (2003) J. Immunol. 170:4881.
14. Lee, K.-M. et al. (2006) Blood 107:3181.
15. McNerney, M.E. et al. (2005) Blood 106:1337.
16. Lee, K.-M. et al. (2004) J. Exp. Med. 199:1245.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD48/SLAMF2 Antibody (AF3327)(3)

Publications using AF3327

• Gang Wu, Gavuthami Murugesan, Manjula Nagala, Alex McCraw, Stuart M. Haslam, Anne Dell et al. Activation of regulatory T cells triggers specific changes in glycosylation associated with Siglec-1-dependent inflammatory responses Wellcome Open Research 2021-06-01 [PMID: 35224210] (Western Blot, Mouse)
• Gang Wu, Gavuthami Murugesan, Manjula Nagala, Alex McCraw, Stuart M. Haslam, Anne Dell et al. Activation of regulatory T cells triggers specific changes in glycosylation associated with Siglec-1-dependent inflammatory responses Wellcome Open Research 6/1/2021 [PMID: 35224210]
• DL Coutu, KD Kokkaliari, L Kunz, T Schroeder Multicolor quantitative confocal imaging cytometry Nat. Methods, 2017-11-13;15(1):39-46. 2017-11-13 [PMID: 29320487] (IHC, Mouse)

Bioinformatics

• Gene Symbol: CD48
• Uniprot:
  • BAE96326()