NSFL1C Antibody - BSA Free

Western Blot: NSFL1C Antibody [NBP2-36529] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NSFL1C antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: NSFL1C Antibody [NBP2-36529] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. For blotting immunoprecipitated NSFL1C, it was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

NSFL1C Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NSFL1C Antibody - BSA Free (NBP2-36529) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by the antibody maps to a region between residue 25 to 75 of human NSFL1 (p97) Cofactor (p47) using the numbering given in entry NP_057227.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NSFL1C
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 10 ug/mg
  • Western Blot 1:2000 - 1:10000
• Application Notes: Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  Validation by IP/Western Blot was performed using a 4-20% SDS-PAGE gel.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NSFL1C Antibody - BSA Free

• dJ776F14.1
• NSFL1 (p97) cofactor (p47)
• NSFL1 cofactor p47
• p47
• p97 cofactor p47
• SHP1 homolog
• UBX domain protein 2C
• UBX domain-containing protein 2C
• UBX1
• UBXD10
• UBXN2CMGC3347

Background

N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NSFL1C
• Uniprot:
  • NP_057227.2(Human)