Notch-1 Antibody Blocking Peptide

• Reactivity: Hu
• Applications: AC

Notch-1 Antibody Blocking Peptide Summary

• Description: A Human Notch-1 antibody blocking peptide
  
  Source: Synthetic (Accession #: P46531)
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Source: Synthetic
• Protein/Peptide Type: Antibody Blocking Peptide
• Gene: NOTCH1
• Purity: >95%

Applications/Dilutions

• Dilutions:
  • Antibody Competition
• Application Notes: Control peptide should be used at 1.0 ug per 1.0 ul of antiserum in per assay. Notch-1 (cleaved N-term) peptide has been tested in dot blot.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: >95%

Alternate Names for Notch-1 Antibody Blocking Peptide

• EC 2.1.2.11
• EC 3.4.21.68
• hN1
• Notch (Drosophila) homolog 1 (translocation-associated)
• notch 1Notch homolog 1, translocation-associated (Drosophila)
• Notch homolog 1, translocation-associated
• Notch1
• Notch-1
• TAN1
• TAN1neurogenic locus notch homolog protein 1
• Translocation-associated notch protein TAN-1

Background

Notch (hN1, Motch in mouse, Xotch in Xenopus) is a family of large transmembrane receptors involved in cell to cell interactions and cell fate decisions during invertebrate and vertebrate development. A modification of Notch signaling pathway can lead to changes in cellular proliferation, differentiation, and apoptotic events (1). Notch is activated by binding to membrane-bound ligands of the Delta and Serrate/Jagged family. Activated Notch receptor is proteolytically cleaved and the Notch intracellular domain (NICD) is released. The NICD binds to CSL DNA-binding protein and the Mastermind/Lag-3 co-activator in the nucleus which activates target gene expression (2,3). Notch has been implicated in T-cell acute lymphoblastic leukemia (T-All) and Multiple Sclerosis (MS). Also, repression of Notch signaling is commonly seen in cancer (4). In mammals, four different Notch receptors has been identified (Notch 1, 2, 3, and 4). Active Notch1 has been linked to inactivation of p53-dependent transactivation by inhibiting the phosphorylation of p53 protein (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for Notch-1 Protein (NB300-251PEP). (Showing 1 - of FAQ).

Bioinformatics

• Gene Symbol: NOTCH1
• Uniprot:
  • P46531()
  • P46531()
  • P46531()
  • P46531()