Noggin Antibody

Western Blot: Noggin Antibody [NBP3-09068] - Western blot analysis of Noggin on Recombinant protein using anti-Noggin antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: Noggin Antibody [NBP3-09068] - ICC staining Noggin in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Noggin Antibody [NBP3-09068] - Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Noggin antibody. Counter stained with hematoxylin.

Flow Cytometry: Noggin Antibody [NBP3-09068] - Flow cytometric analysis of PC-3M cells with Noggin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Immunohistochemistry-Paraffin: Noggin Antibody [NBP3-09068] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Noggin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Noggin Antibody [NBP3-09068] - Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Noggin antibody. Counter stained with hematoxylin.

Product Discontinued

Noggin Antibody Summary

• Immunogen: Recombinant full length protein of Human Noggin. (SwissProt: Q13253 Human; SwissProt: Q62809 Rat)
• Localization: Secreted.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NOG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Noggin Antibody

• NOG
• Noggin
• SYM1
• symphalangism 1 (proximal)
• synostoses (multiple) syndrome 1
• SYNS1
• SYNS1A

Background

Noggin is involved in numerous developmental processes, such as neural tube fusion and joint formation. The morphogenesis of organs is initiated by a downgrowth from a layer of epithelial stem cells. This process is achieved through the receipt of signals from 1) a WNT protein (WNT3A) to stabilize beta-catenin; and 2) Noggin, which is a bone morphogenetic protein inhibitor. Noggin mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) have been identified, which have multiple joint fusion as their principal defect.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NOG