NOD2 Antibody Pack

Images

 
Western Blot: NOD2 Antibody Pack [NB100-935] - Detection of NOD2 in 20ug of NOD2 transfected 293T cell lysate using NB500-253. ECL detection in 15 seconds.
Immunohistochemistry-Frozen: NOD2 Antibody Pack [NB100-935] - Overlay of NOD2-DyLight 488 (NB100-524, green) with phase contrast of murine colon. Image from verified customer review.
Immunohistochemistry-Paraffin: NOD2 Antibody Pack [NB100-935] - Analysis of FFPE human skin using NOD2 antibody (NB500-253) at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat ...read more
Western Blot: NOD2 Antibody Pack [NB100-935] - anti-NOD2 (2D9) Whole cell protein from THP-1 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P, IP
Host
Mouse

Order Details

NOD2 Antibody Pack Summary

Immunogen
See individual datasheets.
Host
Mouse
Gene
NOD2

Applications/Dilutions

Application Notes
See individual datasheets for validated applications.

Reactivity Notes

Human. Other species have not been tested.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Kit Components

Notes

This pack contains 1 vial each of: NB100-524 (0.1 mL) and NB500-253 (0.1 mL).

Alternate Names for NOD2 Antibody Pack

  • BLAU
  • CARD15caspase recruitment domain protein 15
  • caspase recruitment domain family, member 15
  • Caspase recruitment domain-containing protein 15
  • CDACUG
  • CLR16.3
  • IBD1NLR family, CARD domain containing 2
  • Inflammatory bowel disease protein 1
  • NLRC2
  • NOD-like receptor C2
  • nucleotide-binding oligomerization domain 2
  • nucleotide-binding oligomerization domain containing 2
  • nucleotide-binding oligomerization domain, leucine rich repeat and CARD domaincontaining 2
  • nucleotide-binding oligomerization domain-containing protein 2
  • PSORAS1NOD2B

Background

We know how important it is to your research to have access to antibodies specific for your model. As it is often impossible to know which antibody will work best for a specific sample and application, we now offer SuperNovus Packs. These packs include different antibodies to the same protein to give you the ability to decide for yourself. The SuperNovus Packs are all offered at a discount price compared to purchasing each antibody individually. NOD2 is a member of the apoptosis regulating protein family that includes caspase recruitment-domains, as well as Apaf-1 and NOD1. It contains two N-terminal CARDs, a nucleotide binding domain (NBD), and multiple C-terminal leucine-rich repeats (LRRs). NOD2 is expressed in monocytes (whereas NOD1 is expressed in multiple tissues). NOD2 plays a role in regulating NF-kappaB, acts as an intracellular receptor for bacterial lipopolysaccharides, and contributes to inflammatory bowel disease (IBD) and Crohn's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

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Publications for NOD2 Antibody Pack (NB100-935) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NOD2 Antibody Pack (NB100-935). (Showing 1 - 1 of 1 FAQ).

  1. Can you suggest the best NOD2 antibody for detection of endogenous NOD2 in human fibroblasts?
    • For detecting endogenous NOD2 in human samples, I would recommend NB100-524 (http://www.novusbio.com/NB100-524). This antibody has been validated for use in WB, IHC-Paraffin and IP.

Other Available Formats

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Blogs on NOD2.

Transferrin and the blood brain barrier
Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

CD3 (OKT3) as a marker of immune response efficiency
Our immune system is a powerful defense mechanism against infection, however different variables can cause our immune response to work for or against us.  CD3 (cluster of differentiation 3) is one component of our immune signal response that is co...  Read full blog post.

NOD2 - inflammatory signaling and NFkB activation
Nucleotide-binding oligomerization domain-containing protein 2 (NOD2) is an intracellular pattern recognition receptor (PRR) that plays an important role in recognizing bacterial pathogens and initiating an immune response. As a PRR, NOD2 recogniz...  Read full blog post.

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Bioinformatics

Gene Symbol NOD2