NOD2 Antibody (hNOD2.017)

Immunohistochemistry-Paraffin: NOD2 Antibody (hNOD2.017) [NBP3-11219] - NOD2 on human jejunum. Staining of paraffin tissue section with NBP3-11219 with a dilution of 100x.

Immunohistochemistry-Paraffin: NOD2 Antibody (hNOD2.017) [NBP3-11219] - NOD2 on human jejunum. Staining of paraffin tissue section with NBP3-11219 with a dilution of 100x.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP
• Clone: hNOD2.017
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

NOD2 Antibody (hNOD2.017) Summary

• Immunogen: Recombinant NOD2
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: NOD2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunoassay
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: For immunohistochemistry, immunoprecipitation, ELISA and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS, 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for NOD2 Antibody (hNOD2.017)

• BLAU
• CARD15caspase recruitment domain protein 15
• caspase recruitment domain family, member 15
• Caspase recruitment domain-containing protein 15
• CDACUG
• CLR16.3
• IBD1NLR family, CARD domain containing 2
• Inflammatory bowel disease protein 1
• NLRC2
• NOD-like receptor C2
• nucleotide-binding oligomerization domain 2
• nucleotide-binding oligomerization domain containing 2
• nucleotide-binding oligomerization domain, leucine rich repeat and CARD domaincontaining 2
• nucleotide-binding oligomerization domain-containing protein 2
• PSORAS1NOD2B

Background

Monoclonal antibody 2.017 recognizes human NOD2, also known as CARD15. The innate immune system is the first line of defense against pathogens using various pattern recognition receptors (PRR). PRRs include toll-like receptors (TLRs), nucleotide-biding oligomerization domain (NOD)-like receptors (NLRs) and retinoic acid-inducible (RIG)-I-like receptors (RLR). The Nod-like receptor (NLR) family of proteins consists of cytosolic pattern recognition molecules involved in sensing microbial and danger signals and triggering innate immune activation. The NOD family is involved in the regulation of apoptosis and the immune response. The founding members of NLRs are NOD1 and NOD2. These intracellular receptors recognize peptidoglycans. The receptor consists of three main domains. The C-terminal site recognizes the ligand via the leucin-rich repeat domain (LRR). The NOD domain facilitates the self-oligomerization and contains the ATPase activity. The N-terminal site is the caspase-recruitment domain (CARD). Proteoglycans are polymers composed of glycan chains of alternating N-acylglucosamine (GlcNAc) and N-acetylmuramic acid (MurNAc) units cross-linked to each other by short peptides. The cross-linking of two parallel glycan chains is mediated by stem peptides that can be further linked by bridging amino acids. For activation of the immune system, NOD2 senses muramyl dipeptide (MDP), which is found in the PGN of nearly all Gram-positive and Gram-negative organisms, whereas NOD1 recognizes meso-diaminopimelic acid (meso-DAP)-containing PGN fragments. Stimulation of NOD1 or NOD2 results in the activation of NF-kappaB and MAPKs, through a homophilic interaction with the adaptor kinase, Rip2. Thereby driving the transcription of numerous genes involved in both innate and adaptive immune responses. NOD2 seems of special importance in commensal homeostasis and especially in the gut. This is reflected by the fact that genetic variation has been found in patients with inflammatory bowel disease (IBD), particularly Crohn's disease. NOD2 is mainly expressed by macrophages, monocytes as well as Paneth cells, T and B cell, dendritic cells and intestinal epithelial cells. NOD2 antibody 2.017 can be used for western blotting, immunohistochemistry, ELISA and immunoprecipitation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for NOD2 Antibody (NBP3-11219). (Showing 1 - 1 of 1 FAQ).

1. Can you suggest the best NOD2 antibody for detection of endogenous NOD2 in human fibroblasts?
   • For detecting endogenous NOD2 in human samples, I would recommend NB100-524. This antibody has been validated for use in WB, IHC-Paraffin and IP.

Bioinformatics

• Gene Symbol: NOD2