NMT1 Antibody

Western Blot: NMT1 Antibody [NBP3-12963] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NMT1 antibody (NBP3-12963) diluted at 1:5000.

Immunohistochemistry-Paraffin: NMT1 Antibody [NBP3-12963] - NMT1 antibody detects NMT1 protein at cytoplasm in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. NMT1 antibody (NBP3-12963) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: NMT1 Antibody [NBP3-12963] - NMT1 antibody detects NMT1 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. NMT1 antibody (NBP3-12963) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: NMT1 Antibody [NBP3-12963] - NMT1 antibody detects NMT1 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. NMT1 antibody (NBP3-12963) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

NMT1 antibody detects NMT1 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NMT1 protein stained by NMT1 antibody (NBP3-12963) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NMT1 Antibody Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human NMT1. The exact sequence is proprietary.
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NMT1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for NMT1 Antibody

• alternative, short form NMT-S
• glycylpeptide N-tetradecanoyltransferase 1
• long form, NMT-L
• Myristoyl-CoA:protein N-myristoyltransferase 1
• NMT 1
• NMTEC 2.3.1.97
• N-myristoyltransferase 1
• Peptide N-myristoyltransferase 1
• Type I N-myristoyltransferase

Background

Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT; EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1; MIM 139311) (Duronio et al., 1992 (PubMed 1570339)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NMT1