NMT2 Antibody (OTI1G3)

Western Blot: NMT2 Antibody (1G3) [NBP2-01676] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NMT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NMT2.

Immunohistochemistry-Paraffin: NMT2 Antibody (1G3) [NBP2-01676] Staining of paraffin-embedded Human prostate tissue using anti-NMT2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: NMT2 Antibody (1G3) [NBP2-01676] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NMT2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: NMT2 Antibody (1G3) [NBP2-01676] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NMT2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: NMT2 Antibody (1G3) [NBP2-01676] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NMT2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: NMT2 Antibody (1G3) [NBP2-01676] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NMT2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: NMT2 Antibody (1G3) [NBP2-01676] - Staining of paraffin-embedded Human pancreas tissue using anti-NMT2 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC
• Clone: OTI1G3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.69 mg/ml

NMT2 Antibody (OTI1G3) Summary

• Description: Novus Biologicals Mouse NMT2 Antibody (OTI1G3) (NBP2-01676) is a monoclonal antibody validated for use in IHC, WB and Flow. Anti-NMT2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human NMT2(NP_004799) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NMT2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Application Notes: Use in FLOW reported in scientific literature (PMID: 30718913).
• Theoretical MW: 56.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.69 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NMT2 Antibody (OTI1G3)

• EC 2.3.1.97
• glycylpeptide N-tetradecanoyltransferase 2
• Myristoyl-CoA:protein N-myristoyltransferase 2
• NMT 2
• N-myristoyltransferase 2
• Peptide N-myristoyltransferase 2
• Type II N-myristoyltransferase

Background

N-myristoyltransferase (NMT) catalyzes the reaction of N-terminal myristoylation of many signaling proteins. It transfers myristic acid from myristoyl coenzyme A to the amino group of a protein's N-terminal glycine residue. Biochemical evidence indicates the presence of several distinct NMTs, varying in apparent molecular weight and /or subcellular distribution. The predicted 498-amino acid of human NMT2 protein shares 77% and 96% sequence identity with human NMT1 and mouse Nmt2 comprise two distinct families of N-myristoyltransferases. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NMT2 Antibody (NBP2-01676)(1)

Publication using NBP2-01676

• Wen Z, Jin K, Shen Y et al. N-myristoyltransferase deficiency impairs activation of kinase AMPK and promotes synovial tissue inflammation Nat. Immunol. 2019-03-01 [PMID: 30718913] (FLOW, Human)

Bioinformatics

• Gene Symbol: NMT2
• Entrez:
  • 9397(Human)