NMDAR2B [p Tyr1252] Antibody - Azide Free

Western Blot: NMDAR2B [p Tyr1252] Antibody [NB300-292] - Rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit phosphorylated at Tyr1252 (Control). Phosphospecificity is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Tyr1252 NMDA NR2B subunit antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.

Immunohistochemistry: NMDAR2B [p Tyr1252] Antibody [NB300-292] - Immunostaining of 14 DIV rat cortical neurons showing NR2B phosphorylated at Tyr1252 in red and PSD95 in green. Photo courtesy of Gang Liu

• Reactivity: Mu, Rt, Hu, Bv, Ca, Ch, Pm, Ze
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

NMDAR2B [p Tyr1252] Antibody - Azide Free Summary

• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1252 conjugated to KLH. Accession # Q00960
• Modification: p Tyr1252
• Localization: Integral membrane protein.
• Specificity: Specific for endogenous levels of the ~180 kDa NMDAR2B protein phosphorylated at Tyr1252 . Immunolabeling is completely eliminated by treatment with lambda-phosphatase. May also show slight reactivity with Tyr1246 of NR2A.
• Predicted Species: Human (100%), Canine (100%), Chicken (100%), Primate (100%), Zebrafish (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRIN2B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:400
  • Western Blot 1:1000
• Application Notes: This antibody has been tested in Western blot on rat brain hippocampal lysate, detecting a band 180 kDa.
• Theoretical MW: 180 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for NMDAR2B [p Tyr1252] Antibody - Azide Free

• glutamate [NMDA] receptor subunit epsilon-2
• glutamate receptor subunit epsilon-2
• glutamate receptor, ionotropic, N-methyl D-aspartate 2B
• hNR3
• MGC142180
• NMDAR2BMGC142178
• N-methyl D-aspartate receptor subtype 2B
• N-methyl-D-aspartate receptor subunit 3
• NR2B
• NR3

Background

The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has been implicated in several disorders of the CNS including Alzheimer's, epilepsy and ischemic neuronal death. Phosphorylation of Tyr 1252 is thought to potentiate NMDAR dependent influx of calcium. (1-6)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRIN2B
• Entrez:
  • 2904(Human)
  • 24410(Rat)
• Uniprot:
  • Q00960(Rat)