NMDAR2B [p Ser1480] Antibody - Azide Free

Western Blot: NMDAR2B [p Ser1480] Antibody [NB100-61103] - Changes of NMDAR2B phosphorylation levels in the hippocampus and prefrontal cortex of P28 mice after earlylife propofol exposure. Typical results of western blot showed the changes in pSer1480 NMDAR2B phosphorylation levels in cytosol and particulate fractions of the hippocampus and prefrontal cortex of P28 mice after earlylife propofol exposure. D6 mice group treated with propofol (100 mg/kg body weight, i.p.) daily P6-P11; D1 mice group treated with propofil (100 mg/kg, i.p.) at P6 and then normal saline (10 mL/kg, i.p.) from P7-P11; N mice group received normal saline (10 mL/kg, i.p.) from P6-P11. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28729940/) licensed under a CC-BY license.

Western Blot: NMDAR2B [p Ser1480] Antibody [NB100-61103] - Rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Ser1480 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser1480 NMDA NR2B subunit antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.

• Reactivity: Mu, Rt, Hu, Bv, Ca, Ch, Pm, Xp, Ze
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

NMDAR2B [p Ser1480] Antibody - Azide Free Summary

• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser1480 conjugated to KLH. Accession # Q00960
• Modification: p Ser1480
• Specificity: Specific for endogenous levels of the ~180 kDa NMDAR2B protein phosphorylated at Ser1480. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
• Predicted Species: Human (100%), Canine (100%), Chicken (100%), Primate (100%), Xenopus (100%), Bovine (100%), Zebrafish (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRIN2B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 180 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for NMDAR2B [p Ser1480] Antibody - Azide Free

• glutamate [NMDA] receptor subunit epsilon-2
• glutamate receptor subunit epsilon-2
• glutamate receptor, ionotropic, N-methyl D-aspartate 2B
• hNR3
• MGC142180
• NMDAR2BMGC142178
• N-methyl D-aspartate receptor subtype 2B
• N-methyl-D-aspartate receptor subunit 3
• NR2B
• NR3

Background

The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). It has been shown that phosphorylation of Ser1480 disrupts the interaction of NR2B with the PDZ domains of PSD-95 and SAP102 and decreases surface NR2B expression in neurons (Chung et al., 2004).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NMDAR2B Antibody (NB100-61103)(1)

Publication using NB100-61103

• Wang Y, Han S et al. Propofol-induced downregulation of NR2B membrane translocation in hippocampus and spatial memory deficits of neonatal mice. Brain Behav 2017-01-07 [PMID: 28729940] (WB, Mouse)

Bioinformatics

• Gene Symbol: GRIN2B
• Entrez:
  • 24410(Rat)
• Uniprot:
  • Q00960(Rat)