NMDAR2B Antibody (NR2B)

Western Blot: NMDAR2B Antibody (NR2B) [NB100-74475] - Analysis of NMDA receptor type 2B in mouse brain tissue.

Immunohistochemistry-Paraffin: NMDAR2B Antibody (NR2B) [NB100-74475] - Analysis showing staining in the cytoplasm and membrane of human breast tissue (right) compared with a negative control without primary antibody (left).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, ICC/IF, IHC
• Clone: NR2B
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

NMDAR2B Antibody (NR2B) Summary

• Immunogen: Recombinant protein: NR2B subunit of amino acid residues 934-1457.
• Localization: Integral membrane protein.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GRIN2B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:100
  • Simple Western 1:20 - 1:250
  • Western Blot 2 ug/ml
• Application Notes: Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 25815887).
• Theoretical MW: 166 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for NMDAR2B Antibody (NR2B)

• glutamate [NMDA] receptor subunit epsilon-2
• glutamate receptor subunit epsilon-2
• glutamate receptor, ionotropic, N-methyl D-aspartate 2B
• hNR3
• MGC142180
• NMDAR2BMGC142178
• N-methyl D-aspartate receptor subtype 2B
• N-methyl-D-aspartate receptor subunit 3
• NR2B
• NR3

Background

N-methyl-D-aspartate (NMDA) receptors are ligand-gated ion channels that have a high permeability to calcium found in the central nervous system. The receptor consists of a number of distinct ligand binding domains, and the presence of both glutamate and glycine are required for full activation of the channel. Within the channel there is also a binding site for magnesium, which, when occupied, propagates a voltage-dependent channel block. Other binding sites are also found in the receptor, including a zinc-binding site and an inter-channel site that binds specific channel blockers such as phencyclidine (PCP) and related compounds. The NMDA receptor has been demonstrated to play an essential role in long-term potentiation (LTP), a phenomenon that has been implicated to be the basis for learning and memory. The influx of calcium as a result of channel activation is thought to be responsible for neuronal plasticity and glutamate neurotoxicity. A number of different NMDA receptor subunits have been cloned that may possess different functional and localization properties. The NMDA-R1 subunit (NR1) is expressed throughout the brain, while the NMDA-R2 subunits (NR2A, NR2B, NR2C, and NR2D) have a more specific localization pattern. The NMDA receptor subunits differ also in glycine sensitivity, the relative strength of the magnesium channel block, and their respective agonist-dependent deactivation time. Differential splicing of three distinct exons of NR1 generates eight NR1 splice variants. These exons encode a 21 amino acid N-terminal domain (N1) and two 21 amino acid C-terminal domains (C1 and C2, respectively). Splicing out the C2 cassette eliminates a stop codon, generating a new 22 amino acid C-terminal domain (C2'). These splice variants have differing patterns of expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NMDAR2B Antibody (NB100-74475)(5)

Publications using NB100-74475

• Parnell E, Voorn RA, Martin-de-Saavedra MD et al. A developmental delay linked missense mutation in Kalirin-7 disrupts protein function and neuronal morphology Frontiers in Molecular Neuroscience 2022-12-01 [PMID: 36533124] (Western Blot, Rat)
• Allen AT, Heaton EC, Shapiro LP et al. Inter-individual variability amplified through breeding reveals control of reward-related action strategies by Melanocortin-4 Receptor in the dorsomedial striatum Communications biology 2022-02-08 [PMID: 35136204] (WB, Mouse)
• Jiang H, Ye H, Wang Y et al. Anti-N-Methyl-D-Aspartate Receptor Encephalitis Associated with Ovarian Teratoma in South China-Clinical Features, Treatment, Immunopathology, and Surgical Outcomes of 21 Cases Disease Markers 2021-05-21 [PMID: 34093900] (IHC-P, Human)
• Ramberger M, Peschl P, Schanda K et al. Comparison of diagnostic accuracy of microscopy and flow cytometry in evaluating N-methyl-d-aspartate receptor antibodies in serum using a live cell-based assay PLoS ONE 2015-03-30 [PMID: 25815887] (ICC/IF, Human)
• Lopez J, Roffwarg H P et al. Rapid eye movement sleep deprivation decreases long-term potentiation stability and affects some glutamatergic signaling proteins during hippocampal development. Neuroscience 2008-04-22 [PMID: 18359575] (WB, Rat)

Bioinformatics

• Gene Symbol: GRIN2B
• Entrez:
  • 2904(Human)
  • 14812(Mouse)
  • 24410(Rat)
• Uniprot:
  • Q13224(Human)