NMDAR2B Antibody (17) - Chimeric - BSA Free

Flow Cytometry: NMDAR2B Antibody (17) - Chimeric - BSA Free [NBP3-43544] - Paraformaldehyde fixed C6 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-43544 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunocytochemistry /Immunofluorescence: NMDAR2B Antibody (17) - Chimeric - BSA Free [NBP3-43544] - Immunofluorescence analysis of paraformaldehyde fixed C6 cells stained with NBP3-43544 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-43544, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.

• Reactivity: Rt
• Applications: WB
• Clone: 17
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

NMDAR2B Antibody (17) - Chimeric - BSA Free Summary

• Description: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Monoclonal antibodies that bind rat GluN2B ATD were obtained by immunizing mice with the purified intact GluN1-GluN2B NMDA receptors. IgGs were purified from hybridoma cell culture supernatant by Protein-A Sepharose. Fab fragments have been prepared by papain proteolysis followed by re-chromatographing onto Protein-A Sepharose to remove Fc. (Uniprot# Q00960)
• Specificity: Reacts specifically with rat GluN2B ATD.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: Grin2b
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • X-Ray Crystallography

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for NMDAR2B Antibody (17) - Chimeric - BSA Free

• glutamate [NMDA] receptor subunit epsilon-2
• glutamate receptor subunit epsilon-2
• glutamate receptor, ionotropic, N-methyl D-aspartate 2B
• hNR3
• MGC142180
• NMDAR2BMGC142178
• N-methyl D-aspartate receptor subtype 2B
• N-methyl-D-aspartate receptor subunit 3
• NR2B
• NR3

Background

NMDA receptor 2B is an ion channels activated by glutamate. NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors like NMDAR2B also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Grin2b