NLRX1 Antibody

Western Blot: NLRX1 Antibody [NBP2-27198] - Analysis of NLRX1 in mouse heart tissue lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: NLRX1 Antibody [NBP2-27198] - Human heart stained with NLRX1 antibody at 10 ug/ml.

Immunohistochemistry-Paraffin: NLRX1 Antibody [NBP2-27198] - Human brain stained with NLRX1 antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

• Reactivity: Hu, Mu, Rt, Eq, Pm, Bv, Ca
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

NLRX1 Antibody Summary

• Immunogen: A portion of amino acid 580-630 of human NLRX1 was used as the immunogen for the antibody.
• Predicted Species: Canine (95%), Bovine (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NLRX1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5-10ug/ml
  • Western Blot 5-7ug/ml~

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for NLRX1 Antibody

• Caterpiller protein 11.3
• CLR11.3DLNB26
• MGC131937
• MGC21025
• NLR family member X1
• NLR family, X1
• NOD26
• NOD5
• NOD9FLJ21478
• NOD-like receptor X1
• Nucleotide-binding oligomerization domain protein 26
• Nucleotide-binding oligomerization domain protein 5
• Nucleotide-binding oligomerization domain protein 9
• nucleotide-binding oligomerization domain, leucine rich repeat containing X1

Background

NLR family member X1 (NLRX1) belongs to the NLRP family and contains four LRR (leucine-rich) repeats and one NATCH domain. NLRX1 localizes to the mitochondrial outer membrane and interacts through the mitochondrial antiviral signalling adaptor MAVS during viral infection. Expression of NLRX1 results in the potent inhibition of RLH- and MAVS-mediated interferon-beta promoter activity and in the disruption of virus-induced RLH-MAVS interactions. It is also reported that NLRX1 synergistically potentiated ROS production induced by tumour necrosis factor alpha, Shigella infection and double-stranded RNA, resulting in amplified NF-kappaB-dependent and JUN amino-terminal kinases-dependent signalling, thus contributing to the link between ROS generation at the mitochondria and innate immune responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NLRX1
• Uniprot:
  • Q86UT6(Human)