NLRP10/Pynod/NALP10 Antibody - BSA Free

Flow Cytometry: NLRP10/Pynod/NALP10 Antibody [NBP2-76809] - Analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunofluorescence: NLRP10/Pynod/NALP10 Antibody [NBP2-76809] - Analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

• Reactivity: Mu
• Applications: ELISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

NLRP10/Pynod/NALP10 Antibody - BSA Free Summary

• Description: Novus Biologicals Goat NLRP10/Pynod/NALP10 Antibody - BSA Free (NBP2-76809) is a polyclonal antibody validated for use in ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence NDLEENSFKTLKFH-C, from the N Terminus of the protein sequence according to NP_780741.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NLRP10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/ml
  • Immunocytochemistry/ Immunofluorescence
  • Peptide ELISA Detection Limit 1:128000

Reactivity Notes

Expected to react with Rat.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline, pH7.3 with 0.5% bovine serum albumin.
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NLRP10/Pynod/NALP10 Antibody - BSA Free

• CLR11.1
• NACHT, leucine rich repeat and PYD containing 10
• NALP10
• NALP10Pynod
• NLR family, pyrin domain containing 10
• NLRP10
• NOD8
• NOD8Nucleotide-binding oligomerization domain protein 8
• nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domaincontaining 10
• PAN5
• Pynod
• PYNODNACHT, LRR and PYD domains-containing protein 10

Background

Members of the NALP protein family typically contain a NACHT domain, a NACHT-associated domain (NAD), a C-terminalleucine-rich repeat (LRR) region, and an N-terminal pyrin domain (PYD). The protein encoded by this gene belongs tothe NALP protein family despite lacking the LRR region. This protein likely plays a regulatory role in the innateimmune system. The protein belongs to the signal-induced multiprotein complex, the inflammasome, that activates thepro-inflammatory caspases, caspase-1 and caspase-5. Other experiments indicate that this gene acts as amultifunctional negative regulator of inflammation and apoptosis. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NLRP10
• Entrez:
  • 244202(Mouse)
  • 293426(Rat)
• Uniprot:
  • NP_780741.1(Human)