HAF007). A specific band was detected for NLRP10/Pynod/ NALP10 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HT-2 mouse T cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 0.5 µg/mL of Human/Mouse/Rat NLRP10/Pynod/NALP10 Monoclonal Antibody (Catalog # MAB6606) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NLRP10/Pynod/ NALP10 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6606-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85556-25ul | |||
![Western Blot: NLRP10/Pynod/NALP10 Antibody [NBP2-24606] - Analysis of NALP10 in HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) RAW cell lysate using NBP2-24606 at 1.0 ug/ml.](http://images.novusbio.com/fullsize/NLRP10-Pynod-NALP10-Antibody-Western-Blot-NBP2-24606-img0003.jpg "Western Blot: NLRP10/Pynod/NALP10 Antibody [NBP2-24606] - Analysis of NALP10 in HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) RAW cell lysate using NBP2-24606 at 1.0 ug/ml.")
![Western Blot: NLRP10/Pynod/NALP10 Antibody [NBP1-85557] - Analysis in control (vector only transfected HEK293T lysate) and NLRP10 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/NLRP10-Pynod-NALP10-Antibody-Western-Blot-NBP1-85557-img0009.jpg "Western Blot: NLRP10/Pynod/NALP10 Antibody [NBP1-85557] - Analysis in control (vector only transfected HEK293T lysate) and NLRP10 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Flow Cytometry: NLRP10/Pynod/NALP10 Antibody [NBP2-76809] - Analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/NLRP10-Pynod-NALP10-Antibody-Flow-Cytometry-NBP2-76809-img0002.jpg "Flow Cytometry: NLRP10/Pynod/NALP10 Antibody [NBP2-76809] - Analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Immunofluorescence: NLRP10/Pynod/NALP10 Antibody [NBP2-76809] - Analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).](http://images.novusbio.com/fullsize/NLRP10-Pynod-NALP10-Antibody-Immunofluorescence-NBP2-76809-img0001.jpg)
![Western Blot: NLRP10 Overexpression Lysate (Adult Normal) [NBL1-13672] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NLRP10.](http://images.novusbio.com/fullsize/NLRP10-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13672-img0002.jpg "Western Blot: NLRP10 Overexpression Lysate (Adult Normal) [NBL1-13672] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NLRP10.")
