NLRP10/Pynod/NALP10 Antibody (578502) [CoraFluor™ 1]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 578502
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

NLRP10/Pynod/NALP10 Antibody (578502) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: E. coli-derived recombinant human NLRP10/Pynod/NALP10
  Met386-Ile655
  Accession # Q86W26
• Specificity: Detects human NLRP10/Pynod/NALP10 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) NALP1 (aa 1‑323), rhNALP1 (aa 1331‑1429), rhNALP2 (aa 1‑208), rhNALP3 (aa 3‑223), rhNALP3 (aa 540‑689), rhNALP5 (aa 469‑635), rhNOD1 (aa 830‑953), or rhNOD2 (aa 230‑450) is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NLRP10
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for NLRP10/Pynod/NALP10 Antibody (578502) [CoraFluor™ 1]

• CLR11.1
• NACHT, leucine rich repeat and PYD containing 10
• NALP10
• NALP10Pynod
• NLR family, pyrin domain containing 10
• NLRP10
• NOD8
• NOD8Nucleotide-binding oligomerization domain protein 8
• nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domaincontaining 10
• PAN5
• Pynod
• PYNODNACHT, LRR and PYD domains-containing protein 10

Background

Members of the NALP protein family typically contain a NACHT domain, a NACHT-associated domain (NAD), a C-terminalleucine-rich repeat (LRR) region, and an N-terminal pyrin domain (PYD). The protein encoded by this gene belongs tothe NALP protein family despite lacking the LRR region. This protein likely plays a regulatory role in the innateimmune system. The protein belongs to the signal-induced multiprotein complex, the inflammasome, that activates thepro-inflammatory caspases, caspase-1 and caspase-5. Other experiments indicate that this gene acts as amultifunctional negative regulator of inflammation and apoptosis. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NLRP10