NLRP1/NALP1 Antibody - BSA Free

Western Blot: NLRP1/NALP1 Antibody [NBP1-76656] - U937 cell lysate with NALP1 antibody at (A) 1, (B) 2 and (C) 4 ug/ml.

Immunocytochemistry/ Immunofluorescence: NLRP1/NALP1 Antibody - BSA Free [NBP1-76656] - Immunofluorescence of NLRP1/NALP1 in K562 cells with NLRP1/NALP1 antibody at 20 ug/mL.

Immunocytochemistry/ Immunofluorescence: NLRP1/NALP1 Antibody - BSA Free [NBP1-76656] - Immunocytochemistry of NLRP1/NALP1 in K562 cells with NLRP1/NALP1 antibody at 10 ug/mL.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

NLRP1/NALP1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NLRP1/NALP1 Antibody - BSA Free (NBP1-76656) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a peptide corresponding to 13 amino acids near the carboxy terminus of human NALP1. The immunogen is located within the last 50 amino acids of NALP1. Amino Acid Squence: EKGSKKGLLPLSS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NLRP1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10-20 ug/mL
  • Western Blot 1-4 ug/ml
• Control Peptide:

  NLRP1/NALP1 Peptide (NBP1-76656PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Affinity purified

Alternate Names for NLRP1/NALP1 Antibody - BSA Free

• CARD7
• CARD7SLEV1
• Caspase recruitment domain-containing protein 7
• CLR17.1
• Death effector filament-forming ced-4-like apoptosis protein
• DEFCAP
• DEFCAPVAMAS1
• DKFZp586O1822
• KIAA0926DEFCAP-L/S
• NACHT, leucine rich repeat and PYD (pyrin domain) containing 1
• NACHT, leucine rich repeat and PYD containing 1
• NACHT, LRR and PYD containing protein 1
• NACHT, LRR and PYD domains-containing protein 1
• NACPP1044
• NALP1
• NALP1caspase recruitment domain protein 7
• NLR family, pyrin domain containing 1
• NLRP1
• Nucleotide-binding domain and caspase recruitment domain
• nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domaincontaining 1
• PP1044
• SLEV1
• VAMAS1

Background

NALP1 belongs to a family of cytoplasmic proteins that have been implicated in cell responses to apoptotic and inflammatory stimuli. It contains a caspase recruitment domain (CARD) in addition to a NACHT domain and leucine rich repeat (LRR). This protein interacts strongly with caspase 2 and weakly with caspase 9, as well as with Apaf-1. Transient overexpression of this gene in cultured cells was sufficient to induce apoptosis. NALP1 mRNA and protein expression levels in myeloid leukemia cells are increased following CREB (cAMP-response element binding protein) activation, suggesting that NALP1 may contribute to modulate the response of these cells to pro-inflammatory stimuli. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for NALP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NLRP1
• Uniprot:
  • AAG30288(Human)