NKX3.1 Antibody (JE36-27)

Western Blot: NKX3.1 Antibody (JE36-27) [NBP3-32655] - Western blot analysis of NKX3.1 on different lysates with Rabbit anti-NKX3.1 antibody (NBP3-32655) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: RWPE-1 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 26 kDa Observed band size: 30 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32655) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: NKX3.1 Antibody (JE36-27) [NBP3-32655] - Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-NKX3.1 antibody (NBP3-32655) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32655) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: JE36-27
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

NKX3.1 Antibody (JE36-27) Summary

• Description: Novus Biologicals Rabbit NKX3.1 Antibody (JE36-27) (NBP3-32655) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within Human NKX3.1 aa 1-234 / 234. (Uniprot: Q99801)
• Localization: Nucleus.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NKX3-1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:1000
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for NKX3.1 Antibody (JE36-27)

• BAPX2
• BAPX2homeobox protein Nkx-3.1
• Homeobox protein NK-3 homolog A
• NK homeobox (Drosophila), family 3, A
• NK3 homeobox 1
• NK3 transcription factor homolog A
• NK3 transcription factor related, locus 1 (Drosophila)
• NK3 transcription factor related, locus 1
• NKX3.1
• NKX3.1NKX3
• NKX3-1
• NKX3A
• NKX3ANK homeobox, family 3, A

Background

Nkx3.1 is a transcription factor that may play an important role in regulating proliferation of glandular epithelium and in the formation of ducts in the prostate. It has been thought to be one of the target genes of the 8p21 loss of heterozygosity, common in prostate cancer. But neither disruption of the coding region of the gene, nor mutations have been found in prostate cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NKX3-1