Mouse and Rat. Predicted to work in human based on 100% sequence homology.
Alternate Names for NKX3.1 Antibody (0361)
BAPX2homeobox protein Nkx-3.1
Homeobox protein NK-3 homolog A
NK homeobox (Drosophila), family 3, A
NK3 homeobox 1
NK3 transcription factor homolog A
NK3 transcription factor related, locus 1 (Drosophila)
NK3 transcription factor related, locus 1
NKX3ANK homeobox, family 3, A
NKX3.1 (homeobox protein Nkx-3.1) is a homeodomain nuclear transcription factor expressed predominately in the prostate with very low testicular expression and is highly important in prostate biology. NKX3.1 interacts with SRF, SPDEF, WDR77 etc. and TOPORS polyubiquitinates NKX3-1 for inducing its proteasomal degradation regulation. NKX3.1 binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and act as a transcriptional repressor for playing important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Additionally, NKX3.1 functions as a prostate-specific tumor suppressor and diminished NKX3.1 expression is a consistent feature in human/mouse prostate cancers, wherein its expression is lost early in tumorigenesis suggesting that NKX3.1 loss is an early step in the progression to malignant disease.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for NKX3.1 Antibody (NB100-1828). (Showing 1 - 2 of 2 FAQs).
Today we received an inquiry from our customer who was interested in your Nkx3.1 Antibody, #NB100-1828. The customer has a plan to use this antibody in immunohistochemical staining with her paraffin section samples from mouse. The customer would like to have IHC images obtained with #NB100-1828. In addition, the customer would like to know the detailed protocol (antibody concentration, antigen retrieval method, buffer condition and so on) which was used when checking the IHC Application.
Since this is a product we have had around for some time, I was unable to find any data on file for the IHC application as this was reported to us by a customer. I would recommend a standard IHC protocol for staining and a dilution of 1:100. I would recommend antigen retrieval using a heat mediated citrate buffer at a pH 6.0. You can find the general protocol information that our lab recommends here. I hope your customer finds this information useful and again I apologize we do not have any images to send over, but do guarantee this antibody to work for all species and applications listed. The customer may need to directly conjugate the primary antibody to avoid high background with their anti-mouse secondary detecting the mouse samples.
I have three kinds of anti-mouse 2nd Ab; HRP-conjugated anti-mouse Ig G, Ig G2, Ig G2A. Are these all proper for NB100-1828 in WB or IHC?