NKX3.1 Antibody (0361)

Images

 
Western Blot: NKX3.1 Antibody (0361) [NB100-1828] - analysis of NKX3.1 in HeLa cell lysate using anti-NKX3.1 antibody. Image from verified customer review.
Western Blot: Nkx3.1 Antibody (0361) [NB100-1828] - Detection of NKX3.1 in mouse testis lysate using NB 100-1828. ECL detection 1 minute.

Product Details

Summary
Reactivity Mu, Rt, HumanSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P, IP
Clone
0361
Clonality
Monoclonal
Host
Mouse
Concentration
2.3 mg/ml

NKX3.1 Antibody (0361) Summary

Immunogen
Full-length human Nkx3.1 produced in bacteria as a hexahistidine-tagged protein. [UniProt# Q99801]
Localization
Nuclear
Predicted Species
Human (100%). Backed by our 100% Guarantee.
Clonality
Monoclonal
Host
Mouse
Gene
NKX3-1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS (pH 7.4)
Preservative
0.1% Sodium Azide
Concentration
2.3 mg/ml
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
Application Notes
This Nkx3.1 antibody is useful for Immunohistochemistry frozen and paraffin embedded sections, Immunoprecipitation and Western Blot.
Reviewed Applications
Read 2 Reviews rated 4
using
NB100-1828 in the following applications:

Publications
Read Publications using
NB100-1828 in the following applications:

  • 3 publications
  • WB
    2 publications

Reactivity Notes

Mouse and Rat. Predicted to work in human based on 100% sequence homology.

Alternate Names for NKX3.1 Antibody (0361)

  • BAPX2
  • BAPX2homeobox protein Nkx-3.1
  • Homeobox protein NK-3 homolog A
  • NK homeobox (Drosophila), family 3, A
  • NK3 homeobox 1
  • NK3 transcription factor homolog A
  • NK3 transcription factor related, locus 1 (Drosophila)
  • NK3 transcription factor related, locus 1
  • NKX3.1
  • NKX3.1NKX3
  • NKX3-1
  • NKX3A
  • NKX3ANK homeobox, family 3, A

Background

NKX3.1 (homeobox protein Nkx-3.1) is a homeodomain nuclear transcription factor expressed predominately in the prostate with very low testicular expression and is highly important in prostate biology. NKX3.1 interacts with SRF, SPDEF, WDR77 etc. and TOPORS polyubiquitinates NKX3-1 for inducing its proteasomal degradation regulation. NKX3.1 binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and act as a transcriptional repressor for playing important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Additionally, NKX3.1 functions as a prostate-specific tumor suppressor and diminished NKX3.1 expression is a consistent feature in human/mouse prostate cancers, wherein its expression is lost early in tumorigenesis suggesting that NKX3.1 loss is an early step in the progression to malignant disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NKX3.1 Antibody (NB100-1828)(4)

Reviews for NKX3.1 Antibody (NB100-1828) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.
We have 2 reviews tested in 2 application: IHC-P, WB.

Reviews using NB100-1828:
Filter by Applications
IHC-P
(1)
WB
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-P Mouse 04/19/2016
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedProstate tissue
SpeciesMouse
Western Blot NKX3.1 NB100-1828
Enlarge
reviewed by:
Ronald Miller
WB Human 11/20/2014
View

Summary

ApplicationWestern Blot
Sample TestedCell lysate from HeLa cells
SpeciesHuman

Blocking

Blocking Details5% BSA in 1X PBS

Primary Anitbody

Dilution Ratio1:1000 in 1X PBS with troton X-100 (0.1%) at 4 degree O/N

Secondary Antibody

Secondary DescriptionGoat Anti-mouse IgG - HRP (Life Tech)
Secondary Manufacturer Cat#A24524
Secondary Concentration1:5K

Details

Detection NotesECL, 20 seconds exposure with film

Product General Protocols

View specific protocols for NKX3.1 Antibody (NB100-1828): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NKX3.1 Antibody (NB100-1828). (Showing 1 - 2 of 2 FAQs).

  1. Today we received an inquiry from our customer who was interested in your Nkx3.1 Antibody, #NB100-1828. The customer has a plan to use this antibody in immunohistochemical staining with her paraffin section samples from mouse. The customer would like to have IHC images obtained with #NB100-1828. In addition, the customer would like to know the detailed protocol (antibody concentration, antigen retrieval method, buffer condition and so on) which was used when checking the IHC Application.
    • Since this is a product we have had around for some time, I was unable to find any data on file for the IHC application as this was reported to us by a customer. I would recommend a standard IHC protocol for staining and a dilution of 1:100. I would recommend antigen retrieval using a heat mediated citrate buffer at a pH 6.0. You can find the general protocol information that our lab recommends here. I hope your customer finds this information useful and again I apologize we do not have any images to send over, but do guarantee this antibody to work for all species and applications listed. The customer may need to directly conjugate the primary antibody to avoid high background with their anti-mouse secondary detecting the mouse samples.
  2. I have three kinds of anti-mouse 2nd Ab; HRP-conjugated anti-mouse Ig G, Ig G2, Ig G2A. Are these all proper for NB100-1828 in WB or IHC?

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-1828AF405
Alexa Fluor 488 Labeled NB100-1828AF488
Alexa Fluor 647 Labeled NB100-1828AF647
Alexa Fluor 700 Labeled NB100-1828AF700
Biotin Labeled NB100-1828B
DyLight 405 Labeled NB100-1828V
DyLight 488 Labeled NB100-1828G
DyLight 550 Labeled NB100-1828R
DyLight 650 Labeled NB100-1828C
FITC Labeled NB100-1828F
HRP Labeled NB100-1828H

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol NKX3-1
Entrez
Uniprot

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