NKX3.1 Antibody (0361) - BSA Free

Images

 
Western Blot: NKX3.1 Antibody (0361) [NB100-1828] - Analysis of NKX3.1 in HeLa cell lysate using anti-NKX3.1 antibody. WB image submitted by a verified customer review.
Immunohistochemistry-Paraffin: NKX3.1 Antibody (0361) [NB100-1828] - Staining of human chondrosarcoma tissue with NKX3.1 monoclonal antibody. IHC-P image submitted by a verified customer review.
Western Blot: Nkx3.1 Antibody (0361) [NB100-1828] - Detection of NKX3.1 in mouse testis lysate using NB 100-1828. ECL detection 1 minute.
Immunohistochemistry-Paraffin: NKX3.1 Antibody (0361) [NB100-1828] - NKX3.1 was detected in immersion fixed paraffin-embedded sections of mouse testis using Mouse Anti-Mouse NKX3.1 (0361) Monoclonal Antibody (Catalog # ...read more
Genetic Strategies: Knockdown Validated: NKX3.1 Antibody (0361) [NB100-1828] - DWB analysis of NKX3.1 expression in the indicated cells transfected with Ri-Vector (V-Ri) or shRNAs (Ri#1/2) against NKX3.1. Image ...read more
C, D) WB analysis of SALL2 (A) and NKX3-1 (B) expression in the indicated cells transfected with Ri-Vector (V-Ri) or shRNAs (Ri#1/2) against SALL2 or NKX3-1. alpha-Tubulin was used as the loading control. Image ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IP, KD
Clone
0361
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
2.0 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

NKX3.1 Antibody (0361) - BSA Free Summary

Immunogen
Full-length human Nkx3.1 produced in bacteria as a hexahistidine-tagged protein. [UniProt# Q99801]
Localization
Nuclear
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NKX3-1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
  • Western Blot 2 ug/ml
Application Notes
This Nkx3.1 antibody is useful for Immunohistochemistry frozen and paraffin embedded sections, Immunoprecipitation and Western Blot.
Control
NKX3.1 Overexpression Lysate
Mouse Testis Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 3 Reviews rated 4.3
using
NB100-1828 in the following applications:

Publications
Read Publications using
NB100-1828 in the following applications:

  • 3 publications
  • WB
    3 publications

Reactivity Notes

Predicted to work in human based on 100% sequence homology. Human reactivity reported in scientific literature (PMID: 22465996).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.1% Sodium Azide
Concentration
2.0 mg/ml
Purity
Protein G purified

Alternate Names for NKX3.1 Antibody (0361) - BSA Free

  • BAPX2
  • BAPX2homeobox protein Nkx-3.1
  • Homeobox protein NK-3 homolog A
  • NK homeobox (Drosophila), family 3, A
  • NK3 homeobox 1
  • NK3 transcription factor homolog A
  • NK3 transcription factor related, locus 1 (Drosophila)
  • NK3 transcription factor related, locus 1
  • NKX3.1
  • NKX3.1NKX3
  • NKX3-1
  • NKX3A
  • NKX3ANK homeobox, family 3, A

Background

NKX3.1 (homeobox protein Nkx-3.1) is a homeodomain nuclear transcription factor expressed predominately in the prostate with very low testicular expression and is highly important in prostate biology. NKX3.1 interacts with SRF, SPDEF, WDR77 etc. and TOPORS polyubiquitinates NKX3-1 for inducing its proteasomal degradation regulation. NKX3.1 binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and act as a transcriptional repressor for playing important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Additionally, NKX3.1 functions as a prostate-specific tumor suppressor and diminished NKX3.1 expression is a consistent feature in human/mouse prostate cancers, wherein its expression is lost early in tumorigenesis suggesting that NKX3.1 loss is an early step in the progression to malignant disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NKX3.1 Antibody (NB100-1828)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(3)
WB
(3)
All Applications
Filter By Species
Human
(2)
Mouse
(4)
Rat
(1)
All Species

Reviews for NKX3.1 Antibody (NB100-1828) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NB100-1828:
Filter by Applications
IHC-Fr
(1)
IHC-P
(1)
WB
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen NKX3.1 NB100-1828
Enlarge
5
reviewed by:
Verified Customer
IHC-Fr Human 08/04/2021
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedChondrosarcoma
SpeciesHuman
Lot361
  3
reviewed by:
Verified Customer
IHC-P Mouse 04/19/2016
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedProstate tissue
SpeciesMouse
Western Blot NKX3.1 NB100-1828
Enlarge
5
reviewed by:
Ronald Miller
WB Human 11/20/2014
View

Summary

ApplicationWestern Blot
Sample TestedCell lysate from HeLa cells
SpeciesHuman

Product General Protocols

View specific protocols for NKX3.1 Antibody (NB100-1828): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NKX3.1 Antibody (NB100-1828). (Showing 1 - 2 of 2 FAQs).

  1. Today we received an inquiry from our customer who was interested in your Nkx3.1 Antibody, #NB100-1828. The customer has a plan to use this antibody in immunohistochemical staining with her paraffin section samples from mouse. The customer would like to have IHC images obtained with #NB100-1828. In addition, the customer would like to know the detailed protocol (antibody concentration, antigen retrieval method, buffer condition and so on) which was used when checking the IHC Application.
    • Since this is a product we have had around for some time, I was unable to find any data on file for the IHC application as this was reported to us by a customer. I would recommend a standard IHC protocol for staining and a dilution of 1:100. I would recommend antigen retrieval using a heat mediated citrate buffer at a pH 6.0. You can find the general protocol information that our lab recommends here. I hope your customer finds this information useful and again I apologize we do not have any images to send over, but do guarantee this antibody to work for all species and applications listed. The customer may need to directly conjugate the primary antibody to avoid high background with their anti-mouse secondary detecting the mouse samples.
  2. I have three kinds of anti-mouse 2nd Ab; HRP-conjugated anti-mouse Ig G, Ig G2, Ig G2A. Are these all proper for NB100-1828 in WB or IHC?

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Contact Information

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Calculators

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Recent Reviews

4.3
5
2
4
0
3
1
2
0
1
0

Verified Customer
08/04/2021
Application: IHC-Fr
Species: Human

 
Verified Customer
04/19/2016
Application: IHC-P
Species: Mouse

Ronald Miller
11/20/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol NKX3-1