NKX2.5 Antibody

Western Blot: Nkx2.5 Antibody [NBP2-24675] - Western blot analysis of Nkx2.5 in human heart lysate using 1) prebleed and 2) NBP2-24675 at 1 ug/ml.

Immunohistochemistry-Paraffin: Nkx2.5 Antibody [NBP2-24675] - Analysis of NKX2-5 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and NBP2-24675 (bottom left, right) at 5 ug/ml.

• Reactivity: Hu, Pm, Po, Bv, Pm, Eq, Fe, RM
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NKX2.5 Antibody Summary

• Description: Novus Biologicals Rabbit NKX2.5 Antibody (NBP2-24675) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A portion of amino acids 300-324 of human NKX2-5 was used as the immunogen.
• Marker: Cardiac Progenitor Marker
• Predicted Species: Porcine (100%), Chimpanzee (100%), Bovine (100%), Rhesus Macaque (100%), Feline (100%), Equine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NKX2-5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-2 ug/ml

Reactivity Notes

The amino acid sequence used as immunogen is 88% homologous in mouse, rat and opossum.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for NKX2.5 Antibody

• cardiac-specific homeo box
• cardiac-specific homeobox 1
• Cardiac-specific homeobox
• CHNG5
• CSX1
• CSXFLJ97195
• FLJ52202
• FLJ99536
• Homeobox protein CSX
• Homeobox protein NK-2 homolog E
• homeobox protein Nkx-2.5
• NK2 transcription factor related, locus 5 (Drosophila)
• NKX2.5
• NKX2.5FLJ97166
• NKX2-5
• NKX2E
• NKX2EFLJ97197
• NKX4-1
• tinman paralog

Background

Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development (Shiojima et al., 1995 [PubMed 7665173]). It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NKX2-5
• Uniprot:
  • P52952()