NKX2.2 Overexpression Lysate


Western Blot: Nkx2.2 Overexpression Lysate (Adult Normal) [NBL1-13661] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nkx2.2.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

NKX2.2 Overexpression Lysate Summary


NKX2.2 Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC210419

Accession#: NM_002509

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for Array



HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for NKX2.2 Overexpression Lysate

  • Homeobox protein NK-2 homolog B
  • NK-2 (Drosophila) homolog B
  • NK2 homeobox 2
  • NK2 transcription factor related, locus 2 (Drosophila)
  • NK2 transcription factor related, locus 2
  • NK2 transcription factor-like protein B
  • NKX2.2
  • NKX2.2homeobox protein Nkx-2.2
  • NKX2B
  • NKX2BNK-2 homolog B


The protein encoded by this gene contains a homeobox domain and may be involved in the morphogenesis of the central nervous system. This gene is found on chromosome 20 near NKX2-4, and these two genes appear to be duplicated on chromosome 14 in the form of TITF1 and NKX2-8. The encoded protein is likely to be a nuclear transcription factor. [provided by RefSeq].


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Mu
Applications: BA
Species: Hu
Applications: IHC, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, Simple Western, WB
Species: Fi, Hu, Pm, Mu, Pm, Rt, Sh
Applications: ICC/IF, IHC, IP, WB
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
Species: Hu
Applications: ICC, IHC, Simple Western, WB
Species: Hu, Mu, Rt
Applications: ChIP, ICC, IHC, WB
Species: Hu, Mu
Applications: ICC, WB
Species: Hu
Applications: ICC, IHC, Simple Western, WB
Species: Hu
Applications: ICC, WB
Species: Hu
Applications: IHC, IHC-P, WB
Species: Hu
Applications: ChIP, ICC, IHC, WB
Species: Mu
Applications: IHC, WB
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, IF, IHC, IHC-P, WB
Species: Hu, Mu
Applications: CyTOF-ready, ICC, ICFlow
Species: Bv, Ca, Ch, Fe, Gp, Hu, Ma, Mu, Po, Rb, Rt, Sh
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, RI, WB
Species: Hu, Pm, Mu, Rt
Applications: Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu
Applications: IHC, WB
Species: Hu
Applications: ICC, IHC, WB

Publications for NKX2.2 Lysate (NBL1-13661) (0)

There are no publications for NKX2.2 Lysate (NBL1-13661).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for NKX2.2 Lysate (NBL1-13661) (0)

There are no reviews for NKX2.2 Lysate (NBL1-13661). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NKX2.2 Lysate (NBL1-13661) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional NKX2.2 Products

Bioinformatics Tool for NKX2.2 Lysate (NBL1-13661)

Discover related pathways, diseases and genes to NKX2.2 Lysate (NBL1-13661). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NKX2.2 Lysate (NBL1-13661)

Discover more about diseases related to NKX2.2 Lysate (NBL1-13661).

Pathways for NKX2.2 Lysate (NBL1-13661)

View related products by pathway.

PTMs for NKX2.2 Lysate (NBL1-13661)

Learn more about PTMs related to NKX2.2 Lysate (NBL1-13661).

Blogs on NKX2.2

There are no specific blogs for NKX2.2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

NKX2.2 Antibody

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our NKX2.2 Overexpression Lysate and receive a gift card or discount.


Gene Symbol NKX2-2