NKX2.2 Antibody (NX2/294)

Immunohistochemistry-Paraffin: NKX2.2 Antibody (NX2/294) [NBP2-29432] - Formalin-fixed, paraffin-embedded Rat Pancreas stained with NKX2.2 Antibody (NX2/294).

Immunohistochemistry-Paraffin: NKX2.2 Antibody (NX2/294) [NBP2-29432] - Formalin-paraffin pancreas stained with NKX2.2 Ab(NX2/294).

Immunohistochemistry-Frozen: NKX2.2 Antibody (NX2/294) [NBP2-29432] - Analysis of NKX2.2 in human pancreas frozen tissue section using NKX2.2 antibody. Image from verified customer review.

Immunohistochemistry-Paraffin: NKX2.2 Antibody (NX2/294) [NBP2-29432] - Formalin-fixed, paraffin-embedded human Ewing's Sarcoma stained with NKX2.2 Antibody (NX2/294).

• Reactivity: Hu, Mu, Rt, Ch
• Applications: Flow, ICC/IF, IHC
• Clone: NX2/294
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

NKX2.2 Antibody (NX2/294) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33058)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Human full-length recombinant NKX2.2 protein (Uniprot: O95096)
• Localization: Nuclear
• Marker: Neuroendocrine & Ewing's Sarcoma Marker
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NKX2-2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for NKX2.2 Antibody (NX2/294)

• Homeobox protein NK-2 homolog B
• NK-2 (Drosophila) homolog B
• NK2 homeobox 2
• NK2 transcription factor related, locus 2 (Drosophila)
• NK2 transcription factor related, locus 2
• NK2 transcription factor-like protein B
• NKX2.2
• NKX2.2homeobox protein Nkx-2.2
• NKX2B
• NKX2BNK-2 homolog B

Background

The NKX families of homeobox genes are known to act as intermediaries in the neural response to sonic hedgehog signaling during central nervous system development. NKX2.2 is a member of this family of transcription factors and is necessary for neuroendocrine differentiation in the central nervous system and pancreas (Sander M, 2000). This protein was recently identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It also acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93% and a specificity of 89%, and aids in the differential diagnosis of small round cell tumors (Yoshida A, 2012). NKX2.2 preferentially binds the promoter of the alpha cell-specific factor Arx in beta cells when the promoter is in a hypermethylated state. Disruption of the interaction between NKX2.2 and Grgs in mice leads to ectopic expression of Arx in beta cells and is sufficient to cause beta-to-alpha-cell reprogramming during embryonic development and in the adult. These combined in vitro and in vivo studies elucidate how cell-specific DNA modifications and transcriptional regulatory events come together to establish and maintain islet beta cell identity (Papizan JB, 2011).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for NKX2.2 Antibody (NBP2-29432) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IF Human 09/30/2015

Summary

• Application: Immunofluorescence
• Species: Human

Bioinformatics

• Gene Symbol: NKX2-2
• Uniprot:
  • O95096(Human)