A recominant protein fragment specific to NKX2.2 was used as the immunogen for the NX2/294 antibody.
Neuroendocrine & Ewing's Sarcoma Marker
Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93% and a specificity of 89%, and aids in the differential diagnosis of small round cell tumors.
Protein G purified
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Protein G purified
Human Chromosome Location: 20p11.22 Mol. Weight of Antigen: 40-50kDa Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
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Alternate Names for NKX2.2 Antibody (NX2/294)
Homeobox protein NK-2 homolog B
NK-2 (Drosophila) homolog B
NK2 homeobox 2
NK2 transcription factor related, locus 2 (Drosophila)
NK2 transcription factor related, locus 2
NK2 transcription factor-like protein B
NKX2.2homeobox protein Nkx-2.2
NKX2BNK-2 homolog B
The NKX families of homeobox genes are known to act as intermediaries in the neural response to sonic hedgehog signaling during central nervous system development. NKX2.2 is a member of this family of transcription factors and is necessary for neuroendocrine differentiation in the central nervous system and pancreas (Sander M, 2000). This protein was recently identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It also acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93% and a specificity of 89%, and aids in the differential diagnosis of small round cell tumors (Yoshida A, 2012). NKX2.2 preferentially binds the promoter of the alpha cell-specific factor Arx in beta cells when the promoter is in a hypermethylated state. Disruption of the interaction between NKX2.2 and Grgs in mice leads to ectopic expression of Arx in beta cells and is sufficient to cause beta-to-alpha-cell reprogramming during embryonic development and in the adult. These combined in vitro and in vivo studies elucidate how cell-specific DNA modifications and transcriptional regulatory events come together to establish and maintain islet beta cell identity (Papizan JB, 2011).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.