NKp80/KLRF1 Antibody (239127) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: Flow
• Clone: 239127
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 594
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

NKp80/KLRF1 Antibody (239127) [Alexa Fluor® 594] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human NKp80/KLRF1
  Val66-Tyr231
  Accession # Q9NZS2
• Specificity: Detects human NKp80/KLRF1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) NKp30, rhNKp44, rhNKp46, or recombinant mouse NKp46 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: KLRF1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells
• Application Notes: Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
• Preservative: 0.09% Sodium Azide
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKp80/KLRF1 Antibody (239127) [Alexa Fluor® 594]

• Activating coreceptor NKp80
• CLEC5C
• CLEC5CMGC119908
• C-type lectin domain family 5 member C
• killer cell lectin-like receptor F1
• killer cell lectin-like receptor subfamily F member 1
• killer cell lectin-like receptor subfamily F, member 1
• KLRF1
• Lectin-like receptor F1
• MGC119907
• MGC119909
• ML
• NKp80

Background

NKp80, also known as killer cell lectin-like receptor subfamily F member 1 (KLRF1), is an 80 kDa type II transmembrane, homodimeric, activating co‑receptor expressed exclusively on the surface of NK cells. Human NKp80, a member of the C-type lectin family of proteins (1‑3), is 231 aa in length with a 38 amino acid (aa) cytoplasmic region, a 21 aa transmembrane region, and a 172 aa extracellular domain (ECD). The ECD contains the C-type lectin-like domain (aa 114‑230) and four potential sites of N-linked glycosylation. Multiple splicing variants produce four isoforms for NKp80. Isoform 1 is the standard protein. There are no murine orthologs for NKp80 (4). NKp80 functions as an inhibitory receptor based on the presence of two immunoreceptor tyrosine‑based inhibitory motifs (ITIM) in the N-terminal cytoplasmic region and the lack of charged amino acids in the transmembrane region (1). However, later studies show that NKp80 displays an activating function rather than an inhibitory one (2‑3, 5). In in vitro experiments in human NK cells, NKp80 stimulates NK cell cytotoxicity and induces calcium influx after triggering by appropriate antibodies (2, 3). One study reports that NKp80 likely functions as a co‑receptor, cooperating with NKp46 and other activating receptors in NK cell cytotoxicity (2). Activation-induced C-type lectin (AICL) is the ligand for NKp80 (3). It is a myeloid-specific receptor expressed by monocytes, macrophages, and granulocytes (3). Crosslinking of both NKp80 and AICL stimulates secretion of proinflammatory cytokines suggesting that NKp80‑AICL interaction is involved in the activating crosstalk between NK cells and myeloid cells, and thus may influence the initiation and maintenance of immune responses in humans (3).

1. Roda-Navarro, P. et al. (2000) Eur. J. Immunol. 30:568.
2. Vitale, M. et al. (2001) Eur. J. Immunol. 31:233.
3. Welte, S. et al. (2006) Nat. Immunol. 7:1334.
4. Yokoyama, W.M. and B.F. Plougastel (2003) Nat. Rev. Immunol. 3:304.
5. Thomas, M. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105:1656.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KLRF1
• Uniprot:
  • Q9NZS2(Human)