NKp80/KLRF1 Antibody (239127) [Alexa Fluor® 488]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
239127
Clonality
Monoclonal
Host
Rat
Conjugate
Alexa Fluor 488
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

NKp80/KLRF1 Antibody (239127) [Alexa Fluor® 488] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human NKp80/KLRF1
Val66-Tyr231
Accession # Q9NZS2
Specificity
Detects human NKp80/KLRF1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) NKp30, rhNKp44, rhNKp46, or recombinant mouse NKp46 is observed.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
KLRF1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKp80/KLRF1 Antibody (239127) [Alexa Fluor® 488]

  • Activating coreceptor NKp80
  • CLEC5C
  • CLEC5CMGC119908
  • C-type lectin domain family 5 member C
  • killer cell lectin-like receptor F1
  • killer cell lectin-like receptor subfamily F member 1
  • killer cell lectin-like receptor subfamily F, member 1
  • KLRF1
  • Lectin-like receptor F1
  • MGC119907
  • MGC119909
  • ML
  • NKp80

Background

NKp80, also known as killer cell lectin-like receptor subfamily F member 1 (KLRF1), is an 80 kDa type II transmembrane, homodimeric, activating co‑receptor expressed exclusively on the surface of NK cells. Human NKp80, a member of the C-type lectin family of proteins (1‑3), is 231 aa in length with a 38 amino acid (aa) cytoplasmic region, a 21 aa transmembrane region, and a 172 aa extracellular domain (ECD). The ECD contains the C-type lectin-like domain (aa 114‑230) and four potential sites of N-linked glycosylation. Multiple splicing variants produce four isoforms for NKp80. Isoform 1 is the standard protein. There are no murine orthologs for NKp80 (4). NKp80 functions as an inhibitory receptor based on the presence of two immunoreceptor tyrosine‑based inhibitory motifs (ITIM) in the N-terminal cytoplasmic region and the lack of charged amino acids in the transmembrane region (1). However, later studies show that NKp80 displays an activating function rather than an inhibitory one (2‑3, 5). In in vitro experiments in human NK cells, NKp80 stimulates NK cell cytotoxicity and induces calcium influx after triggering by appropriate antibodies (2, 3). One study reports that NKp80 likely functions as a co‑receptor, cooperating with NKp46 and other activating receptors in NK cell cytotoxicity (2). Activation-induced C-type lectin (AICL) is the ligand for NKp80 (3). It is a myeloid-specific receptor expressed by monocytes, macrophages, and granulocytes (3). Crosslinking of both NKp80 and AICL stimulates secretion of proinflammatory cytokines suggesting that NKp80‑AICL interaction is involved in the activating crosstalk between NK cells and myeloid cells, and thus may influence the initiation and maintenance of immune responses in humans (3).

  1. Roda-Navarro, P. et al. (2000) Eur. J. Immunol. 30:568.
  2. Vitale, M. et al. (2001) Eur. J. Immunol. 31:233.
  3. Welte, S. et al. (2006) Nat. Immunol. 7:1334.
  4. Yokoyama, W.M. and B.F. Plougastel (2003) Nat. Rev. Immunol. 3:304.
  5. Thomas, M. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105:1656.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB1900U
Alexa Fluor 405 FAB1900V
Alexa Fluor 488 FAB1900G
Alexa Fluor 594 FAB1900T
Alexa Fluor 647 FAB1900R
Alexa Fluor 700 FAB1900N
Alexa Fluor 750 FAB1900S
Allophycocyanin FAB1900A
Allophycocyanin FAB1900A
Phycoerythrin FAB1900P
Phycoerythrin FAB1900P

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Bioinformatics

Gene Symbol KLRF1
Uniprot