NKp46/NCR1 Antibody (195314) [Unconjugated]

Images

 
NKp46/NCR1 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human NKp46/NCR1 Monoclonal Antibody (Catalog # MAB1850) at 5 µg/mL for 1 hour at room temperature followed ...read more
NKp46/NCR1 was detected in immersion fixed NK‑92 human natural killer lymphoma cell line (left panel, positive stain) and human peripheral blood mononuclear cells (PBMCs; right panel, negative stain) using Mouse ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-reported, Func, ICC/IF
Clone
195314
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

NKp46/NCR1 Antibody (195314) [Unconjugated] Summary

Additional Information
Clone 195314 was used by HLDA to establish CD designation
Immunogen
Mouse T cell hybridoma transfected with human NKp46/NCR1
Specificity
Detects human NKp46/NCR1 in direct ELISAs and Western blots.
Details of Functionality
ActivityAssaywCitation not found None None None None None
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
NCR1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-25 ug/mL
  • Agonist Activity
  • CyTOF-reported
  • Immunocytochemistry 8-25 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB1850 in the following applications:

Publications
Read Publications using
MAB1850 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKp46/NCR1 Antibody (195314) [Unconjugated]

  • CD335 antigen
  • CD335
  • hNKp46
  • Ly94
  • LY94lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46)
  • Lymphocyte antigen 94 homolog
  • MAR-1
  • natural cytotoxicity triggering receptor 1
  • Natural killer cell p46-related protein
  • NCR1
  • NK cell-activating receptor
  • NKp46
  • NKP46FLJ99094
  • NK-p46lymphocyte antigen 94 (mouse) homolog (activating NK-receptor; NK-p46)

Background

NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR) (1). These receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. The CD56dimCD16+ subpopulation that makes up the majority of NK cells in the peripheral blood and spleen expresses NKp46 in both resting and activated states (2). The main NK cell population of the lymph node (CD56brightCD16-) expresses low levels of NKp46 in resting cells, but expression is up-regulated by IL-2. NKp46 is a type I transmembrane protein with two extracellular Ig-like domains followed by a short stalk region, a transmembrane domain containing a positively charged amino acid residue, and a short cytoplasmic tail. Through its positive charge in the transmembrane domain, NKp46 associates with the ITAM‑bearing signal adapter proteins, CD3 zeta and Fc epsilon R1 gamma, which are able to form disulfide-linked homodimers and heterodimers (3, 8). Studies with neutralizing antibodies indicate that the three NCRs are primarily responsible for triggering the NK-mediated lysis of many human tumor cell lines. Blocking any of the NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously (4). NKp46 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (5‑7). Human NKp46 shares 58% and 59% amino acid sequence identity with the mouse and rat proteins, respectively.

  1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255. 
  2. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
  3. Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235. 
  4. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
  5. Arnon, T. et al. (2004) Blood 103:664.
  6. Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.
  7. Mandelboim, O. et al. (2001) Nature 409:1055.
  8. Moretta, A. et al. (2001) Annu. Rev. Immunol. 19:197.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NKp46/NCR1 Antibody (MAB1850)(20)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 7 applications: Epitope Mapping, Functional Assay, ICC, IHC, IHC-Fr, IHC-P, Neutralization.


Filter By Application
Epitope Mapping
(1)
Functional Assay
(4)
ICC
(1)
IHC
(7)
IHC-Fr
(1)
IHC-P
(4)
Neutralization
(1)
All Applications
Filter By Species
Human
(17)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using MAB1850 Applications Species
WN Jin, K Shi, W He, JH Sun, L Van Kaer, FD Shi, Q Liu Neuroblast senescence in the aged brain augments natural killer cell cytotoxicity leading to impaired neurogenesis and cognition Nature Neuroscience, 2020;0(0):. 2020 [PMID: 33257875] (IHC, Mouse) IHC Mouse
K Yamashita, M Iwatsuki, N Yasuda-Yos, T Morinaga, Y Nakao, K Harada, K Eto, J Kurashige, Y Hiyoshi, T Ishimoto, Y Nagai, S Iwagami, Y Baba, Y Miyamoto, N Yoshida, JA Ajani, H Baba Trastuzumab upregulates programmed death ligand-1 expression through interaction with NK cells in gastric cancer Br J Cancer, 2020;0(0):. 2020 [PMID: 33100329] (IHC, Human) IHC Human
AM Trotta, S Santagata, S Zanotta, C D'Alterio, M Napolitano, G Rea, R Camerlingo, F Esposito, E Lamantia, A Anniciello, G Botti, N Longo, G Botti, S Pignata, S Perdonà, S Scala Mutated Von Hippel-Lindau-renal cell carcinoma (RCC) promotes patients specific natural killer (NK) cytotoxicity J. Exp. Clin. Cancer Res., 2018;37(1):297. 2018 [PMID: 30514329] (IHC, Human) IHC Human
SB Maron, L Alpert, HA Kwak, S Lomnicki, L Chase, D Xu, E O'Day, RJ Nagy, RB Lanman, F Cecchi, T Hembrough, A Schrock, J Hart, SY Xiao, N Setia, DVT Catenacci Targeted therapies for targeted populations: Anti-EGFR treatment for EGFR amplified gastroesophageal adenocarcinoma Cancer Discov, 2018;0(0):. 2018 [PMID: 29449271] (IHC, Human) IHC Human
Y Feng, S Liao, C Wei, D Jia, K Wood, Q Liu, X Wang, FD Shi, WN Jin Infiltration and persistence of lymphocytes during late-stage cerebral ischemia in middle cerebral artery occlusion and photothrombotic stroke models J Neuroinflammation, 2017;14(1):248. 2017 [PMID: 29246244] (IHC, Human) IHC Human
J Shi, J Zhao, X Zhang, Y Cheng, J Hu, Y Li, X Zhao, Q Shang, Y Sun, B Tu, L Shi, B Gao, FS Wang, Z Zhang Activated hepatic stellate cells impair NK cell anti-fibrosis capacity through a TGF-?-dependent emperipolesis in HBV cirrhotic patients Sci Rep, 2017;7(0):44544. 2017 [PMID: 28291251] (IHC-Fr, Human) IHC-Fr Human
M Messaouden, G Fregni, D Enot, N Jacquelot, E Neves, N Germaud, HJ Garchon, W Boukouaci, R Tamouza, J Chanal, MF Avril, A Toubert, L Zitvogel, S Rusakiewic, A Caignard NKp30 isoforms and NKp46 transcripts in metastatic melanoma patients: Unique NKp30 pattern in rare melanoma patients with favorable evolution Oncoimmunology, 2016;5(12):e1154251. 2016 [PMID: 28123867] (Functional Assay, Human) Functional Assay Human
Involvement of NK Cells and NKp30 Pathway in Antisynthetase Syndrome J Immunol, 2016;197(5):1621-30. 2016 [PMID: 27511738] (IHC-P, Human) IHC-P Human
Wang S, Xia P, Chen Y, Huang G, Xiong Z, Liu J, Li C, Ye B, Du Y, Fan Z Natural Killer-like B Cells Prime Innate Lymphocytes against Microbial Infection. Immunity, 2016;45(1):131-44. 2016 [PMID: 27421702] (IHC, Human) IHC Human
Hudspeth K, Donadon M, Cimino M, Pontarini E, Tentorio P, Preti M, Hong M, Bertoletti A, Bicciato S, Invernizzi P, Lugli E, Torzilli G, Gershwin M, Mavilio D Human liver-resident CD56(bright)/CD16(neg) NK cells are retained within hepatic sinusoids via the engagement of CCR5 and CXCR6 pathways. J Autoimmun, 0;66(0):40-50. 0 [PMID: 26330348] (IHC-P, Human) IHC-P Human
Show All 20 Publications.

Review for NKp46/NCR1 Antibody (MAB1850) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB1850:
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Human
(1)
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Images Ratings Applications Species Date Details
 NKp46/NCR1 MAB1850
Enlarge
4
reviewed by:
Anonymous
Human 10/05/2020
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Summary

Sample TestedFFPE,human cutaneous squamous cell carcinoma
SpeciesHuman
LotJRA0419111

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
10/05/2020
Application:
Species: Human

Bioinformatics

Gene Symbol NCR1