NKp46/NCR1 Antibody (195314) [Unconjugated] Summary
Clone 195314 was used by HLDA to establish CD designation
Mouse T cell hybridoma transfected with human NKp46/NCR1
Detects human NKp46/NCR1 in direct ELISAs and Western blots.
|Details of Functionality
ActivityAssaywCitation not found None None None None None
Protein A or G purified from hybridoma culture supernatant
<0.10 EU per 1 μg of the antibody by the LAL method.
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Western Blot 1 ug/mL
- Flow Cytometry 2.5 ug/10^6 cells
- Immunohistochemistry 5-25 ug/mL
- Agonist Activity
- Immunocytochemistry 8-25 ug/mL
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MAB1850 in the following applications:
Packaging, Storage & Formulations
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.5 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for NKp46/NCR1 Antibody (195314) [Unconjugated]
- CD335 antigen
- LY94lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46)
- Lymphocyte antigen 94 homolog
- natural cytotoxicity triggering receptor 1
- Natural killer cell p46-related protein
- NK cell-activating receptor
- NK-p46lymphocyte antigen 94 (mouse) homolog (activating NK-receptor; NK-p46)
NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR) (1). These receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. The CD56dimCD16+ subpopulation that makes up the majority of NK cells in the peripheral blood and spleen expresses NKp46 in both resting and activated states (2). The main NK cell population of the lymph node (CD56brightCD16-) expresses low levels of NKp46 in resting cells, but expression is up-regulated by IL-2. NKp46 is a type I transmembrane protein with two extracellular Ig-like domains followed by a short stalk region, a transmembrane domain containing a positively charged amino acid residue, and a short cytoplasmic tail. Through its positive charge in the transmembrane domain, NKp46 associates with the ITAM‑bearing signal adapter proteins, CD3 zeta and Fc epsilon R1 gamma, which are able to form disulfide-linked homodimers and heterodimers (3, 8). Studies with neutralizing antibodies indicate that the three NCRs are primarily responsible for triggering the NK-mediated lysis of many human tumor cell lines. Blocking any of the NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously (4). NKp46 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (5‑7). Human NKp46 shares 58% and 59% amino acid sequence identity with the mouse and rat proteins, respectively.
- Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
- Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
- Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
- Pende, D. et al. (1999) J. Exp. Med. 190:1505.
- Arnon, T. et al. (2004) Blood 103:664.
- Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.
- Mandelboim, O. et al. (2001) Nature 409:1055.
- Moretta, A. et al. (2001) Annu. Rev. Immunol. 19:197.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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