NKp46/NCR1 Antibody (195314) [Fluorescein]

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human NKp46/NCR1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1850F) or (B) Mouse IgG2BFluorescein Isotype Control (Catalog # IC0041F). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 195314
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Fluorescein

NKp46/NCR1 Antibody (195314) [Fluorescein] Summary

• Additional Information: Clone 195314 was used by HLDA to establish CD designation
• Immunogen: Mouse T cell hybridoma transfected with human NKp46/NCR1
• Specificity: Detects human NKp46/NCR1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NCR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKp46/NCR1 Antibody (195314) [Fluorescein]

• CD335 antigen
• CD335
• hNKp46
• Ly94
• LY94lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46)
• Lymphocyte antigen 94 homolog
• MAR-1
• natural cytotoxicity triggering receptor 1
• Natural killer cell p46-related protein
• NCR1
• NK cell-activating receptor
• NKp46
• NKP46FLJ99094
• NK-p46lymphocyte antigen 94 (mouse) homolog (activating NK-receptor; NK-p46)

Background

NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR) (1). These receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. The CD56^dimCD16⁺ subpopulation that makes up the majority of NK cells in the peripheral blood and spleen expresses NKp46 in both resting and activated states (2). The main NK cell population of the lymph node (CD56^brightCD16^-) expresses low levels of NKp46 in resting cells, but expression is up-regulated by IL-2. NKp46 is a type I transmembrane protein with two extracellular Ig-like domains followed by a short stalk region, a transmembrane domain containing a positively charged amino acid residue, and a short cytoplasmic tail. Through its positive charge in the transmembrane domain, NKp46 associates with the ITAM‑bearing signal adapter proteins, CD3 zeta and Fc epsilon R1 gamma , which are able to form disulfide-linked homodimers and heterodimers (3, 8). Studies with neutralizing antibodies indicate that the three NCRs are primarily responsible for triggering the NK-mediated lysis of many human tumor cell lines. Blocking any of the NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously (4). NKp46 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (5‑7). Human NKp46 shares 58% and 59% amino acid sequence identity with the mouse and rat proteins, respectively.

1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255. 
2. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
3. Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235. 
4. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
5. Arnon, T. et al. (2004) Blood 103:664.
6. Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.
7. Mandelboim, O. et al. (2001) Nature 409:1055.
8. Moretta, A. et al. (2001) Annu. Rev. Immunol. 19:197.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NKp46/NCR1 Antibody (FAB1850F)(2)

Publications using FAB1850F

• T Yoshioka, T Tatsumi, T Miyagi, K Mukai, K Nishio, A Nishio, Y Yokoyama, T Suda, T Kegasawa, M Shigekawa, H Hikita, R Sakamori, T Takehara Frequency and role of NKp46 and NKG2A in hepatitis B virus infection PLoS ONE, 2017-03-22;12(3):e0174103. 2017-03-22 [PMID: 28328926] (Flow Cytometry, Human)
• Kwant-Mitchell A, Pek EA, Rosenthal KL Development of functional human NK cells in an immunodeficient mouse model with the ability to provide protection against tumor challenge. PLoS ONE, 2009-12-21;4(12):e8379. 2009-12-21 [PMID: 20027308] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: NCR1