NKp44/NCR2 Antibody (1G6) - BSA Free

Western Blot: NKp44/NCR2 Antibody (1G6) [NBP2-42683] - The Human recombinant proteins, NKp30, NKp44, and NKp46 (each 20ng per well) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NKp44 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunocytochemistry/Immunofluorescence: NKp44/NCR2 Antibody (1G6) [NBP2-42683] - Analysis of NKp44 in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NKp44 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Flow Cytometry: NKp44/NCR2 Antibody (1G6) [NBP2-42683] - Flow cytometry analysis of NKp44 in Jurkat cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 1G6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

NKp44/NCR2 Antibody (1G6) - BSA Free Summary

• Immunogen: Recombinant human NKp44/NCR2 (19-130aa) purified from E. coli
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NCR2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for NKp44/NCR2 Antibody (1G6) - BSA Free

• CD336 antigen
• CD336
• LY95
• LY95lymphocyte antigen 95 homolog (activating NK-receptor; NK-p44)
• lymphocyte antigen 95 (activating NK-receptor; NK-p44)
• Lymphocyte antigen 95 homolog
• natural cytotoxicity triggering receptor 2
• Natural killer cell p44-related protein
• NCR2
• NK cell activating receptor (NKp44)
• NK cell-activating receptor
• NKp44
• NK-p44dJ149M18.1

Background

NKp44 (NCR2) is a member of the natural cytotoxicity receptor (NCR) family that is expressed on activated human NK cells. NKp44 displays a single extracellular Ig-like V domain and a transmembrane portion containing the charged residue (Lysine), likely involved in the association with KARAP/DAP12 molecules. Gene coding for NKp44 maps on human chromosome 6p21.1.This antibody is specific for NKp44 and does not cross react with NKp30 and NKp46.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCR2
• Uniprot:
  • NP_004819()