NKG2D/CD314 Antibody (191004) [Biotin]

NKG2D/CD314 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse NKG2D/CD314 Biotinylated Monoclonal Antibody (Catalog # BAM1547) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Mu
• Applications: WB, ICC/IF
• Clone: 191004
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Biotin
• Concentration: LYOPH

NKG2D/CD314 Antibody (191004) [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse NKG2D/CD314
  Phe94-Val232
  Accession # Q4FJM0
• Specificity: Detects mouse NKG2D/CD314 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: KLRK1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKG2D/CD314 Antibody (191004) [Biotin]

• CD314 antigen
• CD314
• D12S2489E
• FLJ17759
• FLJ75772
• Killer cell lectin-like receptor subfamily K member 1
• killer cell lectin-like receptor subfamily K, member 1
• KLR
• KLRK1
• NK cell receptor D
• NKG2-D type II integral membrane protein
• NKG2D
• NKG2-D
• NKG2-D-activating NK receptor
• NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence

Background

NKG2D, also known as CD314, is a type II transmembrane protein with an extracellular C-type lectin-like domain. It occurs as a disulfide-linked homodimer that associates with the transmembrane DAP10 (DNAX-activator protein 10) adapter protein to deliver an activating signal. This protein shares approximately 25% amino acid sequence identity with a number of other type II lectin-like proteins that are encoded by genes within the natural killer complex on mouse chromosome 6. NKG2D is expressed on NK cells, where it functions as an activating receptor to trigger cytolytic activity and cytokine secretion, and on some T cell subsets, where it acts as a co‑stimulatory receptor complementing T cell receptor signaling. Several ligands have now been identified for mouse NKG2D including H60 and Rae 1 alpha , beta , gamma , δ, and epsilon . All of these ligands are cell-surface proteins distantly related to MHC class I. However, they do not bind peptide or associate with beta 2-microglobulin. Ligand expression is up-regulated in many transformed cell lines and also during conditions of stress such as heat shock or viral infection. In vivo, tumor models demonstrate that NKG2D functions in anti-tumor surveillance (1-5).

1. Bauer, S. et al. (1999) Science 285:727.
2. Wu, J. et al. (1999) Science 285:730.
3. Diefenbach, A. et al. (2001) Nature 413:165.
4. Vivier, E. et al. (2002) Curr. Opin. Immunol. 14:306.
5. NKG2D and its Ligands; www.RnDSystems.com.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NKG2D/CD314 Antibody (BAM1547)(3)

Publications using BAM1547

• Kurt Brassington, Peter Kanellakis, Anh Cao, Ban-Hock Toh, Karlheinz Peter, Alex Bobik et al. Crosstalk between cytotoxic CD8+ T cells and stressed cardiomyocytes triggers development of interstitial cardiac fibrosis in hypertensive mouse hearts Frontiers in Immunology 2022-11-22 [PMID: 36483558]
• Duncan F, Silva K, Johnson C, King B, Szatkiewicz J, Kamdar S, Ong D, Napoli J, Wang J, King L, Whiting D, McElwee K, Sundberg J, Everts H Endogenous retinoids in the pathogenesis of alopecia areata. J Invest Dermatol, 2012-09-27;133(2):334-43. 2012-09-27 [PMID: 23014334] (IHC, Mouse)
• Ogasawara K, Hamerman JA, Ehrlich LR, Bour-Jordan H, Santamaria P, Bluestone JA, Lanier LL NKG2D blockade prevents autoimmune diabetes in NOD mice. Immunity, 2004-06-01;20(6):757-67. 2004-06-01 [PMID: 15189740] (Flow Cytometry, IHC, Mouse)

Review for NKG2D/CD314 Antibody (BAM1547) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer Flow Mouse 01/15/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID: 22942430
• Species: Mouse

Bioinformatics

• Gene Symbol: KLRK1
• Uniprot:
  • Q4FJM0()