NKG2D/CD314 Antibody (191004)


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Product Details

Reactivity MuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready

NKG2D/CD314 Antibody (191004) Summary

Mouse myeloma cell line NS0-derived recombinant mouse NKG2D
Accession # O54709
Detects mouse NKG2D in direct ELISAs and Western blots. Does not cross-react with recombinant human NKG2D.
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.01 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
Read Publications using
MAB1547 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
No Preservative
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKG2D/CD314 Antibody (191004)

  • CD314 antigen
  • CD314
  • D12S2489E
  • FLJ17759
  • FLJ75772
  • Killer cell lectin-like receptor subfamily K member 1
  • killer cell lectin-like receptor subfamily K, member 1
  • KLR
  • KLRK1
  • NK cell receptor D
  • NKG2-D type II integral membrane protein
  • NKG2D
  • NKG2-D
  • NKG2-D-activating NK receptor
  • NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence


NKG2D is a type II transmembrane protein with an extracellular C-type lectin-like domain. It occurs as a disulfide-linked homodimer that associates with the transmembrane DAP10 (DNAX-activator protein 10) adapter protein to deliver an activating signal. This protein shares approximately 25% amino acid sequence identity with a number of other type II lectin-like proteins that are encoded by genes within the natural killer complex on mouse chromosome 6. NKG2D is expressed on NK cells, where it functions as an activating receptor to trigger cytolytic activity and cytokine secretion, and on some T cell subsets, where it acts as a co‑stimulatory receptor complementing T cell receptor signaling. Several ligands have now been identified for mouse NKG2D including H60 and Rae 1 alpha, beta, gamma, δ, and epsilon. All of these ligands are cell-surface proteins distantly related to MHC class I. However, they do not bind peptide or associate with beta 2-microglobulin. Ligand expression is up-regulated in many transformed cell lines and also during conditions of stress such as heat shock or viral infection. In vivo, tumor models demonstrate that NKG2D functions in anti-tumor surveillance (1-5).

  1. Bauer, S. et al. (1999) Science 285:727.
  2. Wu, J. et al. (1999) Science 285:730.
  3. Diefenbach, A. et al. (2001) Nature 413:165.
  4. Vivier, E. et al. (2002) Curr. Opin. Immunol. 14:306.
  5. NKG2D and its Ligands; www.RnDSystems.com.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NKG2D/CD314 Antibody (MAB1547)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Bioassay, Functional Assay, IHC-Fr, Neutralization.

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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using MAB1547 Applications Species
L Qian, Y Liu, S Wang, W Gong, X Jia, L Liu, F Ye, J Ding, Y Xu, Y Fu, F Tian NKG2D ligand RAE1? induces generation and enhances the inhibitor function of myeloid-derived suppressor cells in mice J. Cell. Mol. Med, 2017;0(0):. 2017 [PMID: 28276625] (Neutralization, Mouse) Neutralization Mouse
Ham H, Huynh W, Schoon R, Vale R, Billadeau D HkRP3 is a microtubule-binding protein regulating lytic granule clustering and NK cell killing. J Immunol, 2015;194(8):3984-96. 2015 [PMID: 25762780] (Bioassay, Mouse) Bioassay Mouse
Nielsen M, Dyring-Andersen B, Schmidt J, Witherden D, Lovato P, Woetmann A, Odum N, Poulsen S, Havran W, Geisler C, Bonefeld C NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity. J Invest Dermatol, 2015;135(5):1311-9. 2015 [PMID: 25634359] (Bioassay, Mouse) Bioassay Mouse
Ibusuki A, Kawai K, Yoshida S, Uchida Y, Nitahara-Takeuchi A, Kuroki K, Kajikawa M, Ose T, Maenaka K, Kasahara M, Kanekura T NKG2D triggers cytotoxicity in murine epidermal gammadelta T cells via PI3K-dependent, Syk/ZAP70-independent signaling pathway. J Invest Dermatol, 2014;134(2):396-404. 2014 [PMID: 23962808] (Neutralization, Mouse) Neutralization Mouse
Hosomi S, Chen Z, Baker K, Chen L, Huang Y, Olszak T, Zeissig S, Wang J, Mandelboim O, Beauchemin N, Lanier L, Blumberg R CEACAM1 on activated NK cells inhibits NKG2D-mediated cytolytic function and signaling. Eur J Immunol, 2013;43(9):2473-83. 2013 [PMID: 23696226] (Neutralization, Mouse) Neutralization Mouse
Denoyer A, Godefroy D, Celerier I, Frugier J, Riancho L, Baudouin F, Rostene W, Baudouin C CX3CL1 expression in the conjunctiva is involved in immune cell trafficking during toxic ocular surface inflammation. Mucosal Immunol, 2012;5(0):702. 2012 [PMID: 22692452] (IHC-Fr, Mouse) IHC-Fr Mouse
Li Z, Zhang C, Zhou Z, Zhang J, Zhang J, Tian Z Small intestinal intraepithelial lymphocytes expressing CD8 and T cell receptor gammadelta are involved in bacterial clearance during Salmonella enterica serovar Typhimurium infection. Infect. Immun., 2012;80(2):565-74. 2012 [PMID: 22144492] (Neutralization, Mouse) Neutralization Mouse
Slavuljica I, Busche A, Babic M, Mitrovic M, Gasparovic I, Cekinovic D, Markova Car E, Pernjak Pugel E, Cikovic A, Lisnic VJ, Britt WJ, Koszinowski U, Messerle M, Krmpotic A, Jonjic S Recombinant mouse cytomegalovirus expressing a ligand for the NKG2D receptor is attenuated and has improved vaccine properties. J. Clin. Invest., 2010;120(12):4532-45. 2010 [PMID: 21099111] (Neutralization, Mouse) Neutralization Mouse
Lundqvist A, Yokoyama H, Smith A, Berg M, Childs R Bortezomib treatment and regulatory T-cell depletion enhance the antitumor effects of adoptively infused NK cells. Blood, 2009;113(24):6120-7. 2009 [PMID: 19202127] (Neutralization, Human) Neutralization Human
Takada A, Yoshida S, Kajikawa M, Miyatake Y, Tomaru U, Sakai M, Chiba H, Maenaka K, Kohda D, Fugo K, Kasahara M Two novel NKG2D ligands of the mouse H60 family with differential expression patterns and binding affinities to NKG2D. J. Immunol., 2008;180(3):1678-85. 2008 [PMID: 18209064] (Functional Assay, Mouse) Functional Assay Mouse
Show All 12 Publications.

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Gene Symbol KLRK1