NKG2D/CD314 Antibody (191004)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready
Clone
191004
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

NKG2D/CD314 Antibody (191004) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse NKG2D
Phe94-Val232
Accession # O54709
Specificity
Detects mouse NKG2D in direct ELISAs and Western blots. Does not cross-react with recombinant human NKG2D.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
KLRK1
Purity
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.01 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
Publications
Read Publications using
MAB1547 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKG2D/CD314 Antibody (191004)

  • CD314 antigen
  • CD314
  • D12S2489E
  • FLJ17759
  • FLJ75772
  • Killer cell lectin-like receptor subfamily K member 1
  • killer cell lectin-like receptor subfamily K, member 1
  • KLR
  • KLRK1
  • NK cell receptor D
  • NKG2-D type II integral membrane protein
  • NKG2D
  • NKG2-D
  • NKG2-D-activating NK receptor
  • NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence

Background

NKG2D is a type II transmembrane protein with an extracellular C-type lectin-like domain. It occurs as a disulfide-linked homodimer that associates with the transmembrane DAP10 (DNAX-activator protein 10) adapter protein to deliver an activating signal. This protein shares approximately 25% amino acid sequence identity with a number of other type II lectin-like proteins that are encoded by genes within the natural killer complex on mouse chromosome 6. NKG2D is expressed on NK cells, where it functions as an activating receptor to trigger cytolytic activity and cytokine secretion, and on some T cell subsets, where it acts as a co‑stimulatory receptor complementing T cell receptor signaling. Several ligands have now been identified for mouse NKG2D including H60 and Rae 1 alpha, beta, gamma, δ, and epsilon. All of these ligands are cell-surface proteins distantly related to MHC class I. However, they do not bind peptide or associate with beta 2-microglobulin. Ligand expression is up-regulated in many transformed cell lines and also during conditions of stress such as heat shock or viral infection. In vivo, tumor models demonstrate that NKG2D functions in anti-tumor surveillance (1-5).

  1. Bauer, S. et al. (1999) Science 285:727.
  2. Wu, J. et al. (1999) Science 285:730.
  3. Diefenbach, A. et al. (2001) Nature 413:165.
  4. Vivier, E. et al. (2002) Curr. Opin. Immunol. 14:306.
  5. NKG2D and its Ligands; www.RnDSystems.com.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NKG2D/CD314 Antibody (MAB1547)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Bioassay, Functional Assay, IHC-Fr, Neutralization.


Filter By Application
Bioassay
(2)
Functional Assay
(2)
IHC-Fr
(2)
Neutralization
(6)
All Applications
Filter By Species
Human
(1)
Mouse
(11)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using MAB1547 Applications Species
L Qian, Y Liu, S Wang, W Gong, X Jia, L Liu, F Ye, J Ding, Y Xu, Y Fu, F Tian NKG2D ligand RAE1? induces generation and enhances the inhibitor function of myeloid-derived suppressor cells in mice J. Cell. Mol. Med, 2017;0(0):. 2017 [PMID: 28276625] (Neutralization, Mouse) Neutralization Mouse
Ham H, Huynh W, Schoon R, Vale R, Billadeau D HkRP3 is a microtubule-binding protein regulating lytic granule clustering and NK cell killing. J Immunol, 2015;194(8):3984-96. 2015 [PMID: 25762780] (Bioassay, Mouse) Bioassay Mouse
Nielsen M, Dyring-Andersen B, Schmidt J, Witherden D, Lovato P, Woetmann A, Odum N, Poulsen S, Havran W, Geisler C, Bonefeld C NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity. J Invest Dermatol, 2015;135(5):1311-9. 2015 [PMID: 25634359] (Bioassay, Mouse) Bioassay Mouse
Ibusuki A, Kawai K, Yoshida S, Uchida Y, Nitahara-Takeuchi A, Kuroki K, Kajikawa M, Ose T, Maenaka K, Kasahara M, Kanekura T NKG2D triggers cytotoxicity in murine epidermal gammadelta T cells via PI3K-dependent, Syk/ZAP70-independent signaling pathway. J Invest Dermatol, 2014;134(2):396-404. 2014 [PMID: 23962808] (Neutralization, Mouse) Neutralization Mouse
Hosomi S, Chen Z, Baker K, Chen L, Huang Y, Olszak T, Zeissig S, Wang J, Mandelboim O, Beauchemin N, Lanier L, Blumberg R CEACAM1 on activated NK cells inhibits NKG2D-mediated cytolytic function and signaling. Eur J Immunol, 2013;43(9):2473-83. 2013 [PMID: 23696226] (Neutralization, Mouse) Neutralization Mouse
Denoyer A, Godefroy D, Celerier I, Frugier J, Riancho L, Baudouin F, Rostene W, Baudouin C CX3CL1 expression in the conjunctiva is involved in immune cell trafficking during toxic ocular surface inflammation. Mucosal Immunol, 2012;5(0):702. 2012 [PMID: 22692452] (IHC-Fr, Mouse) IHC-Fr Mouse
Li Z, Zhang C, Zhou Z, Zhang J, Zhang J, Tian Z Small intestinal intraepithelial lymphocytes expressing CD8 and T cell receptor gammadelta are involved in bacterial clearance during Salmonella enterica serovar Typhimurium infection. Infect. Immun., 2012;80(2):565-74. 2012 [PMID: 22144492] (Neutralization, Mouse) Neutralization Mouse
Slavuljica I, Busche A, Babic M, Mitrovic M, Gasparovic I, Cekinovic D, Markova Car E, Pernjak Pugel E, Cikovic A, Lisnic VJ, Britt WJ, Koszinowski U, Messerle M, Krmpotic A, Jonjic S Recombinant mouse cytomegalovirus expressing a ligand for the NKG2D receptor is attenuated and has improved vaccine properties. J. Clin. Invest., 2010;120(12):4532-45. 2010 [PMID: 21099111] (Neutralization, Mouse) Neutralization Mouse
Lundqvist A, Yokoyama H, Smith A, Berg M, Childs R Bortezomib treatment and regulatory T-cell depletion enhance the antitumor effects of adoptively infused NK cells. Blood, 2009;113(24):6120-7. 2009 [PMID: 19202127] (Neutralization, Human) Neutralization Human
Takada A, Yoshida S, Kajikawa M, Miyatake Y, Tomaru U, Sakai M, Chiba H, Maenaka K, Kohda D, Fugo K, Kasahara M Two novel NKG2D ligands of the mouse H60 family with differential expression patterns and binding affinities to NKG2D. J. Immunol., 2008;180(3):1678-85. 2008 [PMID: 18209064] (Functional Assay, Mouse) Functional Assay Mouse
Show All 12 Publications.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol KLRK1
Uniprot