Afamin Antibody

Western blot shows human plasma. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human Afamin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8065) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Afamin at approximately 87 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Afamin Antibody Summary

• Immunogen: Human embryonic kidney cell line HEK293-derived recombinant human Afamin
  Leu22-Asn599
  Accession # P43652
• Specificity: Detects human Afamin in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse Afamin is observed, and less than 1% cross-reactivity with recombinant human (rh) AFB and rhAFP is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: AFM
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Afamin Antibody

• Afamin
• AFM
• ALB2
• ALB2alpha-Alb
• ALBA
• ALBAalpha-albumin
• ALF
• Alpha-Alb
• Alpha-albumin
• MGC125338
• MGC125339

Background

AFM (Afamin; also alpha -Albumin) is a secreted monomeric glycoprotein member of the Alb/albumin family of molecules. Although its MW in SDS-Page ranges from 84-88 kDa, MALDI-TOF analysis yields a likely more correct value of 70-75 kDa. It is expressed by hepatocytes, CNS endothelial cells and osteoclasts, and circulates in the blood at low μg/mL concentrations. AFM is known to bind and transport vitamin E, particularly under conditions where lipoprotein is limited. This is likely to be important in follicular fluid and CSF. It also serves as an osteoclast-derived chemoattractant for preosteoblasts, providing a rational for the observation that bone formation often follows bone resorption. Mature human AFM is 578 amino acids (aa) in length (aa 22-599). It contains three consecutive albumin domains (aa 36-206, 211-403 and 404-599) that contain a characteristic 5 or 6 intrachain disulfide bonds. Full-length human AFM shares 67% aa sequence identity with mouse AFM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Afamin Antibody (AF8065)(1)

Publication using AF8065

• R Yentrapall, J Merl-Pham, O Azimzadeh, L Mutschelkn, C Peters, SM Hauck, MJ Atkinson, S Tapio, S Moertl Quantitative changes in the protein and miRNA cargo of plasma exosome-like vesicles after exposure to ionizing radiation Int. J. Radiat. Biol., 2017-03-07;0(0):1-12. 2017-03-07 [PMID: 28264626] (Western Blot, Human)

Bioinformatics

• Gene Symbol: AFM
• Uniprot:
  • P43652()