beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP1-19788] - Antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP1-19788] - Analysis in mouse brain using DAB with hematoxylin counterstain.

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.11 mg/ml

beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody - BSA Free (NBP1-19788) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to an N-terminal portion of the human B3TGAT1 protein (between residues 1-50) [UniProt Q9P2W7]
• Localization: Golgi apparatus membrane; Single-pass type II membrane protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: B3GAT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:400
• Application Notes: Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.11 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody - BSA Free

• B3GAT1
• beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
• Beta-1,3-glucuronyltransferase 1
• CD57
• EC 2.4.1.135
• galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
• GLCATP
• GlcAT-P
• GLCATPGLCUATP
• GLCUATP
• glcUAT-P
• Glucuronosyltransferase P
• HNK1
• LEU7
• NK1
• NK-1
• UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase

Background

CD57 (also known as beta-1,3-glucuronyltransferase 1, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, Glucuronosyltransferase P, Human Natural Killer 1, B3GAT1, NK1, HNK1, LEU7, NK-1, GLCUATP, and GLCATP) is a member of the glucuronyltransferase gene family. The enzymes produced by the members of this family recognize non-reducing terminal sugars and their anomeric linkages by exhibiting strong acceptor specificity. CD57 is also involved in the biosynthesis of glycosaminoglycan and L2/HNK-1 carbohydrate epitopes on glycoproteins. CD57 is expressed mainly in the brain and several alternate transcriptional splice variants have been characterized. In cancer research, CD57 positive lymphocytes have been used successfully in monitoring the progression of various cancers (PMID: 21491089). HNK1 is also involved in synaptic plasticity and neurodevelopment (PMID: 20950796).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody (NBP1-19788)(1)

Publication using NBP1-19788

• Tsuda M, Ambrosini YM, Zhang W, Yang GX, Ando Y, Rong G, Tsuneyama K, Sumida K, Shimoda S, Bowlus CL, Leung PS, He XS, Coppel RL, Ansari AA, Lian ZX, Gershwin ME. Fine phenotypic and functional characterization of effector cluster of differentiation 8 positive T cells in human patients with primary biliary cirrhosis. Hepatology;54(4):1293-302. 2011-10-01 [PMID: 21735469] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: B3GAT1
• Entrez:
  • 27087(Human)
• Uniprot:
  • Q9P2W7()