Niemann-Pick type C1 Like-1 Antibody

Images

 
Western Blot: Niemann-Pick type C1 Like-1 Antibody [NB400-127] - WB detection of NPC1L1 protein in lysate of human small intestine tissue using Niemann-Pick type C1 Like-1 antibody at 2ug/ml concentration.
Immunohistochemistry: Niemann-Pick type C1 Like-1 Antibody [NB400-127] - Staining of Niemann-Pick type C1 Like-1 in mouse intestine using NB400-127.

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, EM, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Concentration
1.0 mg/ml

Niemann-Pick type C1 Like-1 Antibody Summary

Immunogen
A synthetic peptide made to an internal region of rat Niemann-Pick type C1 Like-1 (between residues 1000-1100). [UniProt# Q6T3U3]
Clonality
Polyclonal
Host
Rabbit
Gene
NPC1L1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Conjugate
Unconjugated
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Electron Microscopy
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100-1:250
  • Immunohistochemistry-Paraffin 1:100-1:250
Application Notes
This Niemann-Pick type C1 Like-1 antibody is useful for Immunohistochemistry paraffin embedded sections and Western blot. In Western blot a band is observed at ~140 kDa representing NPC1L1, and a nonspecific band may be observed at ~45 kDa depending on the lysate used. Use in Electron Microscopy reported in scientific literature (PMID: 21051527) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Niemann-Pick type C1 Like-1 Peptide (NB400-127PEP)
Publications
Read Publications using
NB400-127 in the following applications:

  • EM
    1 publication
  • 2 publications
  • 1 publication
  • WB
    6 publications

Reactivity Notes

Human, rat, pig and mouse.

Alternate Names for Niemann-Pick type C1 Like-1 Antibody

  • Niemann-Pick C1-like protein 1
  • NPC1 (Niemann-Pick disease, type C1, gene)-like 1
  • NPC11L1

Background

Dietary consumption and intestinal cholesterol absorption contribute to plasma cholesterol levels, which factor into the risk of coronary heart disease. Niemann-Pick type C1 Like 1 protein (NPC1L1 or NPC3) is enriched in the small intestine and is found in the brush border membrane of enterocytes. It plays a critical role in absorption of intestinal cholesterol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Niemann-Pick type C1 Like-1 Antibody (NB400-127)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 4 applications: EM, ICC/IF, IHC-P, WB.


Filter By Application
EM
(1)
ICC/IF
(2)
IHC-P
(1)
WB
(6)
All Applications
Filter By Species
Human
(3)
Mouse
(1)
Porcine
(2)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB400-127 Applications Species
Park Y, Carr TPet al. Unsaturated fatty acids and phytosterols regulate cholesterol transporter genes in Caco-2 and HepG2 cell lines. Nutr Res. 2013 Feb [PMID:23399666] (WB, Human) WB Human
Grenier E, Garofalo C, Delvin E, Levy E. Modulatory Role of PYY in Transport and Metabolism of Cholesterol in Intestinal Epithelial Cells plos One 2012 [PMID:22844422] (WB, Human) WB Human
Schmitz M, Signore SC, Zerr I, Althaus HH. Oligodendroglial Process Formation is Differentially Affected by Modulating the Intra- and Extracellular Cholesterol Content J Mol Neurosci 2012 Jun 28 [PMID:22740150] (ICC/IF, WB, Porcine) ICC/IF, WB Porcine
Skov M, Tonnesen CK, Hansen GH et al. Dietary cholesterol induces trafficking of intestinal Niemann-Pick Type C1 Like 1 from the brush border to endosomes. Am J Physiol Gastrointest Liver Physiol 2011 Jan [PMID:21051527] (WB, IHC-P, EM, Porcine) WB, IHC-P, EM Porcine
Nomura M, Ishii H, Kawakami A et al. Inhibition of Hepatic Neiman-Pick C1-Like 1 Improves Hepatic Insulin Resistance. Am J Physiol Endocrinol Metab :00343. 2009 [PMID:19654286]
Alvaro A, Rosales R, Masana L et al. Polyunsaturated fatty acids down-regulate in vitro expression of the key intestinal cholesterol absorption protein NPC1L1: no effect of monounsaturated nor saturated fatty acids. J Nutr Biochem 2010 Jun [PMID:19443194]
Kaufmann, AM, Krise, JP. Niemann-Pick C1 functions in regulating lysosomal amine content. J Biol Chem;283(36):24584-93. 2008 Sep 5. [PMID:18591242] (WB, ICC/IF, Human) WB, ICC/IF Human
Montoudis, A et al. Intestinal fatty acid binding protein regulates mitochondrion beta-oxidation and cholesterol uptake. J Lipid Res;49(5):961-72. 2008 May. [PMID:18235139]
Knopfel M, Davies JP, Duong PT et al. Multiple plasma membrane receptors but not NPC1L1 mediate high-affinity, ezetimibe-sensitive cholesterol uptake into the intestinal brush border membrane. Biochim Biophys Acta;1771(9):1140-7. 2007 Sep. [PMID:17689140] (WB, Mouse) WB Mouse

Reviews for Niemann-Pick type C1 Like-1 Antibody (NB400-127) (0)

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Product General Protocols

View specific protocols for Niemann-Pick type C1 Like-1 Antibody (NB400-127): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Niemann-Pick type C1 Like-1 Antibody (NB400-127). (Showing 1 - 1 of 1 FAQs).

  1. I am currently working with samples from pigs and wants to investigate the cholesterol transporter NPC1L1 by Western blot. For pig, I could not find a specific antibody, so I look for a sequence homology with the pig. Can you give me information on the antigens of the following antibodies: Niemann-Pick type C1 Like-1 Antibody NB400-127 and NB400-128?
    • I ran a blast on the human protein and the pig protein and there is 80% homology between the two proteins. Our lab only recommends using if the percentage homology is 85% or above. You may wish to test either antibody and you would be eligble for our Innovators Reward Program in which you would gain a 50% refund on the product and a 50% discount on another product. All you would need to do is email your results, (successful or unsuccesful) to innovators@novusbio.com. The amino acid range for the immunogen for NB400-127 is 1000-1100 amino acids and that for NB400-128 is 500- 600.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB400-127AF405
Alexa Fluor 488 Labeled NB400-127AF488
Alexa Fluor 647 Labeled NB400-127AF647
Alexa Fluor 700 Labeled NB400-127AF700
Biotin Labeled NB400-127B
DyLight 405 Labeled NB400-127V
DyLight 488 Labeled NB400-127G
DyLight 550 Labeled NB400-127R
DyLight 650 Labeled NB400-127C
FITC Labeled NB400-127F
HRP Labeled NB400-127H

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol NPC1L1

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