Niemann-Pick type C1 Like-1 Antibody - BSA Free

Western Blot: Niemann-Pick type C1 Like-1 Antibody [NB400-127] - Detection of NPC1L1 protein in lysate of human small intestine tissue using Niemann-Pick type C1 Like-1 antibody at 2ug/ml concentration.

Immunocytochemistry/Immunofluorescence: Niemann-Pick type C1 Like-1 Antibody [NB400-127] - HepG2 cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Niemann-Pick type C1 Like-1 Antibody (NB400-127) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemistry-Paraffin: Niemann-Pick type C1 Like-1 Antibody [NB400-127] - Analysis of a FFPE tissue section of mouse small intestine using 1:200 dilution of NPC1L1 antibody (NB400-127). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

• Reactivity: Hu, Mu, Rt, Po
• Applications: WB, ELISA, ICC/IF, IHC, MS, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Niemann-Pick type C1 Like-1 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to an internal region of rat Niemann-Pick type C1 Like-1 (between residues 1000-1100). [UniProt# Q6T3U3]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NPC1L1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Electron Microscopy reported in scientific literature (PMID 21051527)
  • ELISA reported in scientific literature (PMID 21051527)
  • Immunocytochemistry/ Immunofluorescence 2 ug/ml
  • Immunohistochemistry 1:100-1:250
  • Immunohistochemistry-Paraffin 1:100-1:250
  • Knockdown Validated reported in scientific literature (PMID 35023619)
  • Western Blot 2 ug/ml
• Application Notes: In Western blot a band is observed at ~140 kDa representing NPC1L1, and a nonspecific band may be observed at ~45 kDa depending on the lysate used.
• Theoretical MW: 140 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  Niemann-Pick type C1 Like-1 Protein (NB400-127PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Niemann-Pick type C1 Like-1 Antibody - BSA Free

• Niemann-Pick C1-like protein 1
• NPC1 (Niemann-Pick disease, type C1, gene)-like 1
• NPC11L1

Background

Dietary consumption and intestinal cholesterol absorption contribute to plasma cholesterol levels, which factor into the risk of coronary heart disease. Niemann-Pick type C1 Like 1 protein (NPC1L1 or NPC3) is enriched in the small intestine and is found in the brush border membrane of enterocytes. It plays a critical role in absorption of intestinal cholesterol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Niemann-Pick type C1 Like-1 Antibody (NB400-127)(18)

Publications using NB400-127

• Lee C, Kwak SH, Han J et al. Repositioning of ezetimibe for the treatment of idiopathic pulmonary fibrosis. The European respiratory journal 2024-05-16 [PMID: 38359963]
• Zhang Z, Qin S, Chen Y et al. Inhibition of NPC1L1 disrupts adaptive responses of drug-tolerant persister cells to chemotherapy EMBO molecular medicine 2022-01-13 [PMID: 35023619] (KD, Human)
• Haghikia A, Zimmermann F, Schumann P Et al. Propionate attenuates atherosclerosis by immune-dependent regulation of intestinal cholesterol metabolism European heart journal 2021-10-01 [PMID: 34597388] (IHC-P)
• Sugimoto S, Kobayashi E, Fujii M et al. An organoid-based organ-repurposing approach to treat short bowel syndrome Nature 2021-02-24 [PMID: 33627870]
• Zimmermann F, Roessler J, Schmidt D et al. Impact of the Gut Microbiota on Atorvastatin Mediated Effects on Blood Lipids J Clin Med 2020-05-25 [PMID: 32466086] (IF/IHC, Mouse)
• Nihei W, Nagafuku M, Hayamizu H et al. NPC1L1-dependent intestinal cholesterol absorption requires ganglioside GM3 in membrane microdomains. J. Lipid Res. 2018-09-21 [PMID: 30242108] (IF/IHC, Mouse)
• Feng D, Zou J, Zhang S et al. Hypocholesterolemic Activity of Curcumin is Mediated by Down-Regulating the Expression of Niemann-Pick C1-Like 1 in Hamsters. J. Agric. Food Chem. 2016-12-21 [PMID: 28000447]
• Kikuchi T, Orihara K, Oikawa F et al. Intestinal CREBH overexpression prevents high-cholesterol diet-induced hypercholesterolemia by reducing Npc1l1 expression. Mol Metab. 2016-11-01 [PMID: 27818935] (WB, Mouse)
• Sachdev V, Leopold C, Bauer R et al. Novel role of a triglyceride-synthesizing enzyme: DGAT1 at the crossroad between triglyceride and cholesterol metabolism. Molecular and Cell Biology of Lipids. 2016-06-23 [PMID: 27344248]
  
  Details:
  This citation used the Alexa Fluor 488 version of this antibody.
• Park Y, Carr TPet al. Unsaturated fatty acids and phytosterols regulate cholesterol transporter genes in Caco-2 and HepG2 cell lines. Nutr Res. 2013-02-01 [PMID: 23399666] (WB, Human)
• Grenier E, Garofalo C, Delvin E, Levy E. Modulatory Role of PYY in Transport and Metabolism of Cholesterol in Intestinal Epithelial Cells plos One 2012-01-01 [PMID: 22844422] (WB, Human)
• Schmitz M, Signore SC, Zerr I, Althaus HH. Oligodendroglial Process Formation is Differentially Affected by Modulating the Intra- and Extracellular Cholesterol Content J Mol Neurosci 2012-06-28 [PMID: 22740150] (ICC/IF, WB, Porcine)
• Skov M, Tonnesen CK, Hansen GH et al. Dietary cholesterol induces trafficking of intestinal Niemann-Pick Type C1 Like 1 from the brush border to endosomes. Am J Physiol Gastrointest Liver Physiol 2011-01-01 [PMID: 21051527] (IHC-P, EM, WB, Porcine)
• Nomura M, Ishii H, Kawakami A et al. Inhibition of Hepatic Neiman-Pick C1-Like 1 Improves Hepatic Insulin Resistance. Am J Physiol Endocrinol Metab :00343. 2009-01-01 [PMID: 19654286]
• Alvaro A, Rosales R, Masana L et al. Polyunsaturated fatty acids down-regulate in vitro expression of the key intestinal cholesterol absorption protein NPC1L1: no effect of monounsaturated nor saturated fatty acids. J Nutr Biochem 2010-06-01 [PMID: 19443194]
• Kaufmann, AM, Krise, JP. Niemann-Pick C1 functions in regulating lysosomal amine content. J Biol Chem;283(36):24584-93. 2008-09-05 [PMID: 18591242] (ICC/IF, WB, Human)
• Montoudis A, Seidman E, Boudreau F et al. Intestinal fatty acid binding protein regulates mitochondrion beta-oxidation and cholesterol uptake. J Lipid Res;49(5):961-72. 2008-05-01 [PMID: 18235139]
• Knopfel M, Davies JP, Duong PT et al. Multiple plasma membrane receptors but not NPC1L1 mediate high-affinity, ezetimibe-sensitive cholesterol uptake into the intestinal brush border membrane. Biochim Biophys Acta;1771(9):1140-7. 2007-09-01 [PMID: 17689140] (WB, Mouse)

FAQs for Niemann-Pick type C1 Like-1 Antibody (NB400-127). (Showing 1 - 1 of 1 FAQs).

1. I am currently working with samples from pigs and wants to investigate the cholesterol transporter NPC1L1 by Western blot. For pig, I could not find a specific antibody, so I look for a sequence homology with the pig. Can you give me information on the antigens of the following antibodies: Niemann-Pick type C1 Like-1 Antibody NB400-127 and NB400-128?
   • I ran a blast on the human protein and the pig protein and there is 80% homology between the two proteins. Our lab only recommends using if the percentage homology is 85% or above. You may wish to test either antibody and you would be eligble for our Innovators Reward Program in which you would gain a 50% refund on the product and a 50% discount on another product. All you would need to do is email your results, (successful or unsuccesful) to innovators@novusbio.com. The amino acid range for the immunogen for NB400-127 is 1000-1100 amino acids and that for NB400-128 is 500- 600.

Bioinformatics

• Gene Symbol: NPC1L1