Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) - Azide and BSA Free

Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) - Azide and BSA Free [NBP2-72984] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NNMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NNMT.

Immunohistochemistry: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) - Azide and BSA Free [NBP2-72984] - Staining of paraffin-embedded Human pancreas tissue using anti-NNMT mouse monoclonal antibody.

Flow Cytometry: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) - Azide and BSA Free [NBP2-72984] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NNMT.

Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) - Azide and BSA Free [NBP2-72984] - Analysis of extracts (35ug) from 9 different cell lines by using anti-NNMT monoclonal antibody.

Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) - Azide and BSA Free [NBP2-72984] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells and NNMT-Knockout HeLa cells were separated by SDS-PAGE and immunoblotted with anti-NNMT monoclonal antibody (1:500). Then the blotted membrane was stripped and reprobed with anti

Immunohistochemistry: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) - Azide and BSA Free [NBP2-72984] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NNMT mouse monoclonal antibody.

Immunohistochemistry: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) - Azide and BSA Free [NBP2-72984] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NNMT mouse monoclonal antibody.

Immunohistochemistry: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) - Azide and BSA Free [NBP2-72984] - Staining of paraffin-embedded Human endometrium tissue using anti-NNMT mouse monoclonal antibody.

Immunohistochemistry: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) - Azide and BSA Free [NBP2-72984] - Staining of paraffin-embedded Human liver tissue using anti-NNMT mouse monoclonal antibody.

Immunohistochemistry: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) - Azide and BSA Free [NBP2-72984] - Staining of paraffin-embedded Human prostate tissue using anti-NNMT mouse monoclonal antibody.

Flow Cytometry: Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) - Azide and BSA Free [NBP2-72984] - Analysis of Jurkat cells, using anti-NNMT antibody (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready, KO
• Clone: OTI3D8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human NNMT(NP_006160) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NNMT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Knockout Validated
  • Western Blot 1:500-2000
• Theoretical MW: 29.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Nicotinamide N-Methyltransferase/NNMT Antibody (OTI3D8) - Azide and BSA Free

• EC 2.1.1.1
• Nicotinamide NMethyltransferase
• Nicotinamide N-Methyltransferase
• NNMT

Background

N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NNMT