![Western Blot: MTHFD1 Antibody [NBP1-82612] - Analysis using Anti-MTHFD1 antibody NBP1-82612 (A) shows similar pattern to independent antibody NBP1-82611 (B).](http://images.novusbio.com/fullsize2/MTHFD1-Antibody-Western-Blot-NBP1-82612-img0012.jpg "Western Blot: MTHFD1 Antibody [NBP1-82612] - Analysis using Anti-MTHFD1 antibody NBP1-82612 (A) shows similar pattern to independent antibody NBP1-82611 (B).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC, IHC-P, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: LNGKEISAQIRARLKNQVTQLKEQVPGFTPRLAILQVGNRDDSNLYINVKLKAAEEIGIKATHIKLPRTTTESEVMKYITSLNEDSTVHGFLVQLPLDSENSINTEEVINAIAPEKDVDGLTSINAGRLARGDLNDCFIPCT |
| Marker | Cytoplasm Marker |
| Specificity | Specificity of human, mouse, rat MTHFD1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MTHFD1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Publication using NBP1-82612 | Applications | Species |
|---|---|---|
| Tyleckova J, Hrabakova R, Mairychova K et al. Cancer Cell Response to Anthracyclines Effects: Mysteries of the Hidden Proteins Associated with These Drugs. Int J Mol Sci 2012 Nov 22 [PMID: 23443080] |
Secondary Antibodies |
Isotype Controls |
Diseases for MTHFD1 Antibody (NBP1-82612)Discover more about diseases related to MTHFD1 Antibody (NBP1-82612).
| Pathways for MTHFD1 Antibody (NBP1-82612)View related products by pathway.
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PTMs for MTHFD1 Antibody (NBP1-82612)Learn more about PTMs related to MTHFD1 Antibody (NBP1-82612).
| Research Areas for MTHFD1 Antibody (NBP1-82612)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | MTHFD1 |
![Immunohistochemistry-Paraffin: MTHFD1 Antibody [NBP1-82612] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/MTHFD1-Antibody-Immunohistochemistry-Paraffin-NBP1-82612-img0017.jpg "Immunohistochemistry-Paraffin: MTHFD1 Antibody [NBP1-82612] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in glandular cells.")
![Western Blot: MTHFD1 Antibody [NBP1-82612] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize2/MTHFD1-Antibody-Western-Blot-NBP1-82612-img0009.jpg "Western Blot: MTHFD1 Antibody [NBP1-82612] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Western Blot: MTHFD1 Antibody [NBP1-82612] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1, using anti-MTHFD1 antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize2/MTHFD1-Antibody-Western-Blot-NBP1-82612-img0011.jpg "Western Blot: MTHFD1 Antibody [NBP1-82612] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1, using anti-MTHFD1 antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.")
![Immunohistochemistry-Paraffin: MTHFD1 Antibody [NBP1-82612] - Staining of human pancreas shows moderate to strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize2/MTHFD1-Antibody-Immunohistochemistry-Paraffin-NBP1-82612-img0014.jpg "Immunohistochemistry-Paraffin: MTHFD1 Antibody [NBP1-82612] - Staining of human pancreas shows moderate to strong cytoplasmic positivity in exocrine glandular cells.")
![Immunohistochemistry-Paraffin: MTHFD1 Antibody [NBP1-82612] - Staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize2/MTHFD1-Antibody-Immunohistochemistry-Paraffin-NBP1-82612-img0015.jpg "Immunohistochemistry-Paraffin: MTHFD1 Antibody [NBP1-82612] - Staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.")
![Immunohistochemistry-Paraffin: MTHFD1 Antibody [NBP1-82612] - Staining of human fallopian tube shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/MTHFD1-Antibody-Immunohistochemistry-Paraffin-NBP1-82612-img0016.jpg "Immunohistochemistry-Paraffin: MTHFD1 Antibody [NBP1-82612] - Staining of human fallopian tube shows weak to moderate cytoplasmic positivity in glandular cells.")
![Western Blot: MTHFR Antibody (5D3) [NBP2-37607] - Western blot analysis using MTHFR mouse mAb against Rat Heart cell lysate.](http://images.novusbio.com/fullsize2/MTHFR-Antibody-(5D3)-Western-Blot-NBP2-37607-img0003.jpg)
![Immunohistochemistry: MTHFR Antibody (5D3) [NBP2-37607] - Immunohistochemical analysis of paraffin-embedded lung cancer using MTHFR mouse mAb with DAB staining.](http://images.novusbio.com/fullsize2/MTHFR-Antibody-(5D3)-Immunohistochemistry-NBP2-37607-img0004.jpg)
![Western Blot: MTHFR Antibody (5D3) [NBP2-37607] - Western blot analysis using MTHFR mAb against HEK293 (1) and MTHFR(AA: 339-499)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize2/MTHFR-Antibody-(5D3)-Western-Blot-NBP2-37607-img0002.jpg)
![Western Blot: MTRR Antibody [NBP1-57749] - Antibody Titration: 1 ug/ml Positive control: Hela cell lysate.](http://images.novusbio.com/fullsize2/MTRR-Antibody-Western-Blot-NBP1-57749-img0004.jpg)
![Immunohistochemistry: MTRR Antibody [NBP1-57749] - Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue Observed Staining: Cytoplasmic Primary Antibody Concentration: N/A Other Working Concentrations: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec](http://images.novusbio.com/fullsize2/MTRR-Antibody-Immunohistochemistry-NBP1-57749-img0005.jpg)
![Western Blot: MTRR Antibody [NBP1-57749] - HepG2 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize2/MTRR-Antibody-Western-Blot-NBP1-57749-img0002.jpg)
![Immunohistochemistry-Paraffin: MTR Antibody [NB100-791] - Paraffin embedded human adrenal gland. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize2/MTR-Antibody-Immunohistochemistry-Paraffin-NB100-791-img0002.jpg)
![Western Blot: SHMT1 Antibody [NBP2-32173] - Samples: Whole cell lysate (50 ug) from NIH3T3 cells. Antibodies: Affinity purified rabbit anti-SHMT1 antibody NBP2-32173 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize2/SHMT1-Antibody-Western-Blot-NBP2-32173-img0002.jpg)
![Immunohistochemistry: SHMT1 Antibody [NBP2-32173] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- SHMT1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize2/SHMT1-Antibody-Immunohistochemistry-NBP2-32173-img0005.jpg)
![Western Blot: SHMT1 Antibody [NBP2-32173] - Samples: Whole cell lysate (50 ug) from Jurkat cells. Antibodies: Affinity purified rabbit anti-SHMT1 NBP2-32173 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize2/SHMT1-Antibody-Western-Blot-NBP2-32173-img0001.jpg)
![Western Blot: CBS Antibody (3E1) [H00000875-M01] - CBS monoclonal antibody (M01), clone 3E1. Analysis of CBS expression in MCF-7.](http://images.novusbio.com/fullsize2/CBS-Antibody-(3E1)-Western-Blot-H00000875-M01-img0015.jpg)
![Immunohistochemistry-Paraffin: CBS Antibody (3E1) [H00000875-M01] - Analysis of monoclonal antibody to CBS on formalin-fixed paraffin-embedded human hepatocellular carcinoma. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize2/CBS-Antibody-(3E1)-Immunohistochemistry-Paraffin-H00000875-M01-img0010.jpg)
![Western Blot: CBS Antibody (3E1) [H00000875-M01] - Analysis of CBS over-expressed 293 cell line, cotransfected with CBS Validated Chimera RNAi ( Cat # H00000875-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CBS monoclonal antibody (M01), clone 3E1 (Cat # H00000875-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize2/CBS-Antibody-(3E1)-Western-Blot-H00000875-M01-img0012.jpg)
![Western Blot: SHMT2 Antibody [NBP1-80754] - Analysis using Anti-SHMT2 antibody NBP1-80754 (A) shows similar pattern to independent antibody NBP1-80755 (B).](http://images.novusbio.com/fullsize2/SHMT2-Antibody-Western-Blot-NBP1-80754-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: SHMT2 Antibody [NBP1-80754] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/SHMT2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80754-img0009.jpg)
![Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP1-80754] - Staining of human kidney.](http://images.novusbio.com/fullsize2/SHMT2-Antibody-Immunohistochemistry-Paraffin-NBP1-80754-img0025.jpg)
![Western Blot: BHMT Antibody [NBP1-00161] - Rat Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize2/BHMT-Antibody-Western-Blot-NBP1-00161-img0003.jpg)
![Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-00161] - Human Kidney tissue. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize2/BHMT-Antibody-Immunohistochemistry-Paraffin-NBP1-00161-img0005.jpg)
![Western Blot: BHMT Antibody [NBP1-00161] - HEK293 overexpressing BHMT and probed with this antibody (mock transfection in first lane).](http://images.novusbio.com/fullsize2/BHMT-Antibody-Western-Blot-NBP1-00161-img0001.jpg)
![Western Blot: TEF1 Antibody [NBP2-56713] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.](http://images.novusbio.com/fullsize2/TEF1 Antibody-Western Blot-NBP2-56713-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TEF1 Antibody [NBP2-56713] - Staining of human cell line A549 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize2/TEF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56713-img0001.jpg)
![Western Blot: MTHFD1L Antibody [NBP2-37864] - Analysis using Anti-MTHFD1L antibody NBP2-37864 (A) shows similar pattern to independent antibody NBP2-37863 (B).](http://images.novusbio.com/fullsize2/MTHFD1L-Antibody-Western-Blot-NBP2-37864-img0008.jpg)
![Immunohistochemistry-Paraffin: MTHFD1L Antibody [NBP2-37864] - Staining in human placenta and skeletal muscle tissues.. Corresponding MTHFD1L RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/MTHFD1L-Antibody-Immunohistochemistry-Paraffin-NBP2-37864-img0007.jpg)
![Immunohistochemistry-Paraffin: MTHFD1L Antibody [NBP2-37864] - Staining of human skeletal muscle shows weak positivity in myocytes.](http://images.novusbio.com/fullsize2/MTHFD1L-Antibody-Immunohistochemistry-Paraffin-NBP2-37864-img0003.jpg)
![Western Blot: Dihydrofolate Reductase/DHFR Antibody (2B10) [H00001719-M01] - DHFR monoclonal antibody (M01), clone 2B10. Analysis of DHFR expression in PC-12.](http://images.novusbio.com/fullsize2/Dihydrofolate-Reductase-DHFR-Antibody-(2B10)-Western-Blot-H00001719-M01-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Dihydrofolate Reductase/DHFR Antibody (2B10) [H00001719-M01] - Analysis of monoclonal antibody to DHFR on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize2/Dihydrofolate-Reductase-DHFR-Antibody-(2B10)-Immunocytochemistry-Immunofluorescence-H00001719-M01-img0012.jpg)
![Western Blot: Dihydrofolate Reductase/DHFR Antibody (2B10) [H00001719-M01] - DHFR monoclonal antibody (M01), clone 2B10 Analysis of DHFR expression in HeLa.](http://images.novusbio.com/fullsize2/Dihydrofolate-Reductase-DHFR-Antibody-(2B10)-Western-Blot-H00001719-M01-img0014.jpg)
![Western Blot: FUZ Antibody [NBP2-24750] - analysis of FUZ in human lung lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse lung and 4) rat lung using this antibody. 0 ug/ml, 2.0 ug/ml and 6.0 ug/ml respectively.](http://images.novusbio.com/fullsize2/FUZ Antibody-Western Blot-NBP2-24750-img0001.jpg)
![Immunohistochemistry-Paraffin: FUZ Antibody [NBP2-24750] - Formalin-fixed, paraffin-embedded human lung stained with FUZ antibody at 15 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize2/FUZ-Antibody-Immunohistochemistry-Paraffin-NBP2-24750-img0003.jpg)
![Western Blot: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - PSMA expression in human prostate cancer cell lines. Image from verified customer review.](http://images.novusbio.com/fullsize2/PSMA-FOLH1-NAALADase-I-Antibody-(GCP-04)-Western-Blot-NBP1-45057-img0010.jpg)
![Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - GCPII in porcine kidney by GCP-04 monoclonal antibody. Highly positive proximal glomeruli.](http://images.novusbio.com/fullsize2/PSMA-FOLH1-NAALADase-I-Antibody-(GCP-04)-Immunohistochemistry-Paraffin-NBP1-45057-img0008.jpg)
![Western Blot: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - Fragment of human GCPII (aminoacids 44-750) produced in S2 cells on Western blot by GCP-04 monoclonal antibody. Lanes 1, 2, 3 represent 800, 400 and 200 pg of the protein.](http://images.novusbio.com/fullsize2/PSMA-FOLH1-NAALADase-I-Antibody-(GCP-04)-Western-Blot-NBP1-45057-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: GLS2 Antibody [NBP1-76544] - of GLS2 in Human Kidney cells. Dilution 20 ug/mL.](http://images.novusbio.com/fullsize2/GLS2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76544-img0007.jpg)
![Immunohistochemistry: GLS2 Antibody [NBP1-76544] - Immunohistochemistry of GLS2 in human kidney tissue with GLS2 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize2/GLS2-Antibody-Immunohistochemistry-NBP1-76544-img0003.jpg)
![Simple Western: GLS2 Antibody [NBP1-76544] - Simple Western lane view shows a specific band for GLS2 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize2/GLS2-Antibody-Simple-Western-NBP1-76544-img0008.jpg)
![Immunohistochemistry-Paraffin: AGA Antibody [NBP1-88866] - Staining in human epididymis and skeletal muscle tissues using anti-AGA antibody. Corresponding AGA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/AGA-Antibody-Immunohistochemistry-Paraffin-NBP1-88866-img0006.jpg)
![Immunohistochemistry-Paraffin: AGA Antibody [NBP1-88866] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize2/AGA-Antibody-Immunohistochemistry-Paraffin-NBP1-88866-img0002.jpg)
![Immunohistochemistry-Paraffin: AGA Antibody [NBP1-88866] - Staining of human epididymis shows high expression.](http://images.novusbio.com/fullsize2/AGA-Antibody-Immunohistochemistry-Paraffin-NBP1-88866-img0004.jpg)
![Western Blot: PEMT Antibody [NBP1-59580] - 721_B cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize2/PEMT-Antibody-Western-Blot-NBP1-59580-img0005.jpg)
![Western Blot: PEMT Antibody [NBP1-59580] - Rat liver lysate](http://images.novusbio.com/fullsize2/PEMT-Antibody-Western-Blot-NBP1-59580-img0003.jpg)
![Western Blot: PEMT Antibody [NBP1-59580] - Rat liver lysate.](http://images.novusbio.com/fullsize2/PEMT-Antibody-Western-Blot-NBP1-59580-img0004.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")