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MTHFD1 Antibody

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Western Blot: MTHFD1 Antibody [NBP1-82612] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1, using anti-MTHFD1 antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.
Genetic Strategies: Western Blot: MTHFD1 Antibody [NBP1-82612] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1, using anti-MTHFD1 antibody. Remaining relative intensity is ...read more
Independent Antibodies: Western Blot: MTHFD1 Antibody [NBP1-82612] - Analysis using Anti-MTHFD1 antibody NBP1-82612 (A) shows similar pattern to independent antibody NBP1-82611 (B).
Immunohistochemistry-Paraffin: MTHFD1 Antibody [NBP1-82612] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in glandular cells.
Western Blot: MTHFD1 Antibody [NBP1-82612] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: MTHFD1 Antibody [NBP1-82612] - Staining of human pancreas shows moderate to strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: MTHFD1 Antibody [NBP1-82612] - Staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: MTHFD1 Antibody [NBP1-82612] - Staining of human fallopian tube shows weak to moderate cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

    

Genetic Strategies

    

Order Details

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MTHFD1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LNGKEISAQIRARLKNQVTQLKEQVPGFTPRLAILQVGNRDDSNLYINVKLKAAEEIGIKATHIKLPRTTTESEVMKYITSLNEDSTVHGFLVQLPLDSENSINTEEVINAIAPEKDVDGLTSINAGRLARGDLNDCFIPCT
Marker
Cytoplasm Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MTHFD1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
MTHFD1 Protein (NBP1-82612PEP)
Publications
Read Publication using NBP1-82612.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23443080)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MTHFD1 Antibody

  • C1-THF synthase
  • EC 6.3.4.3
  • methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolatecyclohydrolase, 10-formyltetrahydrofolate synthetase
  • methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1
  • MTHFCC-1-tetrahydrofolate synthase, cytoplasmic
  • MTHFDcytoplasmic C-1-tetrahydrofolate synthase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ELISA: MTHFR Antibody (5D3) [NBP2-37607] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
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Immunohistochemistry-Paraffin: SHMT1 Antibody [NBP1-85437] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: SHMT1 Antibody [NBP1-85437] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
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Western Blot: CBS Antibody (3E1) [H00000875-M01] - Analysis of CBS expression in transfected 293T cell line by CBS monoclonal antibody (M01), clone 3E1.Lane 1: CBS transfected lysate(61 KDa).Lane 2: Non-transfected lysate.
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Western Blot: SHMT2 Antibody [NBP1-80755] - Analysis using Anti-SHMT2 antibody NBP1-80755 (A) shows similar pattern to independent antibody NBP1-80754 (B).
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Immunohistochemistry: GLS2 Antibody [NBP1-76544] - Immunohistochemistry of GLS2 in human kidney tissue with GLS2 antibody at 5 ug/mL.
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Western Blot: PEMT Antibody [NBP1-59580] - Rat liver lysate
Western Blot: PEMT Antibody [NBP1-59580] - Rat liver lysate.
Western Blot: PEMT Antibody [NBP1-59580] - 721_B cell lysate, concentration 0.2-1 ug/ml.
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Publications for MTHFD1 Antibody (NBP1-82612)(1)


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Bioinformatics

Gene Symbol MTHFD1