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MTHFD1 Antibody

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Western Blot: MTHFD1 Antibody [NBP1-82612] - Analysis using Anti-MTHFD1 antibody NBP1-82612 (A) shows similar pattern to independent antibody NBP1-82611 (B).
Independent Antibodies: Western Blot: MTHFD1 Antibody [NBP1-82612] - Analysis using Anti-MTHFD1 antibody NBP1-82612 (A) shows similar pattern to independent antibody NBP1-82611 (B).
Immunohistochemistry-Paraffin: MTHFD1 Antibody [NBP1-82612] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in glandular cells.
Western Blot: MTHFD1 Antibody [NBP1-82612] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Genetic Strategies: Western Blot: MTHFD1 Antibody [NBP1-82612] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1, using anti-MTHFD1 antibody. Remaining relative intensity is ...read more
Immunohistochemistry-Paraffin: MTHFD1 Antibody [NBP1-82612] - Staining of human pancreas shows moderate to strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: MTHFD1 Antibody [NBP1-82612] - Staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: MTHFD1 Antibody [NBP1-82612] - Staining of human fallopian tube shows weak to moderate cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

    

Genetic Strategies

    

Order Details

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MTHFD1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LNGKEISAQIRARLKNQVTQLKEQVPGFTPRLAILQVGNRDDSNLYINVKLKAAEEIGIKATHIKLPRTTTESEVMKYITSLNEDSTVHGFLVQLPLDSENSINTEEVINAIAPEKDVDGLTSINAGRLARGDLNDCFIPCT
Marker
Cytoplasm Marker
Specificity
Specificity of human, mouse, rat MTHFD1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MTHFD1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
MTHFD1 Protein (NBP1-82612PEP)
Publications
Read Publication using NBP1-82612.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23443080)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MTHFD1 Antibody

  • C1-THF synthase
  • EC 6.3.4.3
  • methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolatecyclohydrolase, 10-formyltetrahydrofolate synthetase
  • methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1
  • MTHFCC-1-tetrahydrofolate synthase, cytoplasmic
  • MTHFDcytoplasmic C-1-tetrahydrofolate synthase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: MTHFR Antibody (5D3) [NBP2-37607] - Analysis of intima cancer tissues (left) and prostate tissues (right) using MTHFR mouse mAb with DAB staining.
Western Blot: MTHFR Antibody (5D3) [NBP2-37607] - Western blot analysis using MTHFR mAb against HEK293 (1) and MTHFR(AA: 339-499)-hIgGFc transfected HEK293 (2) cell lysate.
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Immunohistochemistry: MTRR Antibody [NBP1-57749] - Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue Observed Staining: Cytoplasmic Primary Antibody Concentration: N/A Other Working Concentrations: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec
Western Blot: MTRR Antibody [NBP1-57749] - HepG2 cell lysate, concentration 0.2-1 ug/ml.
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Western Blot: MTR Antibody [NB100-791] - Cgl null mice exhibit female-specific alteration of enzymes involved in the remethylation of methionine. Western blotting analysis of hepatic BHMT, MTHFR and MTR protein expression levels in male and female WT and Cgl null mice. Values shown represent the mean+-s.d., *Pless than 0.05, **Pless than 0.01, ***Pless than 0.001. Image collected and cropped by CiteAb from the following publication (//bio.biologists.org/cgi/doi/10.1242/bio.013433) licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: MTR Antibody [NB100-791] - Human adrenal gland. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Immunohistochemistry-Paraffin: SHMT1 Antibody [NBP2-32173] - Section of mouse teratoma. Antibody: Affinity purified rabbit anti- SHMT1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
Western Blot: SHMT1 Antibody [NBP2-32173] - Samples: Whole cell lysate (50 ug) from Jurkat cells. Antibodies: Affinity purified rabbit anti-SHMT1 NBP2-32173 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
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Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (SPM453) [NBP2-34390] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Thymidylate Synthase Antibody (SPM453).
Flow (Intracellular): Thymidylate Synthase Antibody (SPM453) [NBP2-34390] - An intracellular stain was performed on Hek293 cells with Thymidylate Synthase Antibody [SPM453] NBP2-34441PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin. Image using the PE format of this antibody.
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[Unconjugated]
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FUZ Antibody
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PSMA/FOLH1/NAALADase I ...
NBP1-45057
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Immunocytochemistry/Immunofluorescence: GLS2 Antibody [NBP1-76544] - of GLS2 in Human Kidney cells. Dilution 20 ug/mL.
Immunohistochemistry: GLS2 Antibody [NBP1-76544] - Immunohistochemistry of GLS2 in human kidney tissue with GLS2 antibody at 5 ug/mL.
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Recombinant Human
AGA Protein
NBP2-52631
SDS-Page: Recombinant Human AGA Protein [NBP2-52631]
(1 Publication)
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PEMT Antibody
NBP1-59580
Western Blot: PEMT Antibody [NBP1-59580] - 721_B cell lysate, concentration 0.2-1 ug/ml.
Western Blot: PEMT Antibody [NBP1-59580] - Rat liver lysate
Western Blot: PEMT Antibody [NBP1-59580] - Rat liver lysate.
Species: Hu, Rt
Applications: WB
MTHFD1 Antibody
NBP1-82612
MTHFD1 Antibody
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, KD

Publications for MTHFD1 Antibody (NBP1-82612)(1)


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Bioinformatics

Gene Symbol MTHFD1