MTR Antibody

Images

 
Western Blot: MTR Antibody [NB100-791] - Staining of Hela lysate (RIPA buffer, 1.4E5 cells per lane). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: MTR Antibody [NB100-791] - Staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citratebuffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

MTR Antibody Summary

Immunogen
Peptide with sequence C-VEKWLGPILGYDTD corresponding to C-Terminus according to NP_000245.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MTR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Immunohistochemistry 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 150 kDa band observed in HeLa and Jurkat lysates. IHC-P: Human adrenal gland shows cytoplasm staining in the cortex.
Positive Control
Jurkat Lysate (NB800-PC2)
HeLa Lysate (NB800-PC1)
Reviewed Applications
Read 1 Review rated 5
using
NB100-791 in the following applications:

Publications
Read Publications using
NB100-791 in the following applications:

  • WB
    1 publication

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine. This batch is not expected to cross-react with Mouse, Rat and Pig Heart in Western blot.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MTR Antibody

  • 5-methyltetrahydrofolate-homocysteine methyltransferase 1
  • 5-methyltetrahydrofolate-homocysteine methyltransferase
  • cblG
  • cobalamin-dependent methionine synthase
  • EC 2.1.1.13
  • FLJ33168
  • FLJ43216,5-methyltetrahydrofolate--homocysteine methyltransferase
  • FLJ45386
  • methionine synthase
  • MS
  • Vitamin-B12 dependent methionine synthase

Background

This enzyme catalyzes the final step in methionine biosynthesis. MTR mutations have been identified as an underlying cause of methylcobalamin deficiency complementation group G.Principle Names - MTR; 5-methyltetrahydrofolate-homocysteine methyltransferase methionine synthase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-37607
Species: Hu, Rt
Applications: WB, ELISA, IHC
NBP1-57749
Species: Hu
Applications: WB, IHC
H00000875-M01
Species: Hu, Rt
Applications: WB, ELISA, ICC/IF, IHC-P, IP, RNAi
NBP1-83936
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-34390
Species: Hu
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP
NBP1-59904
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-32173
Species: Hu, Mu
Applications: WB, ELISA, IHC-P, IP
NBP2-03417
Species: Hu
Applications: WB, Flow, IHC, IHC-P
AF7895
Species: Hu
Applications: WB, IHC
NBP2-43581
Species: Hu
Applications: WB, ICC/IF, IP
NB100-230
Species: Hu
Applications: WB, IP, PLA
NBP1-82612
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-02349
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-80754
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
H00001719-M01
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF
NBP2-56762
Species: Hu
Applications: WB, ICC/IF
NBP1-89285
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-37574
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC

Publications for MTR Antibody (NB100-791)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Mouse
(1)
All Species

Review for MTR Antibody (NB100-791) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NB100-791:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot MTR NB100-791
Enlarge
reviewed by:
Anonymous
WB Mouse 07/14/2012
View

Summary

ApplicationWestern Blot
Sample Testedmouse liver
SpeciesMouse
LotP2
CommentsThis antibody is very good for WB using in mouse liver lysate. The big arrow indicate the MTR band.

Blocking

Blocking Details5% milk in PBS pH 7.4, 0.2% tween 20

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionRabbit anti-Goat IgG Fluorescein
Secondary Manufacturer Cat#31509 from Thermo Sci.
Secondary Concentration1:200

Details

Detection NotesTyphoon

Comments

CommentsThis antibody is very good for WB using in mouse liver lysate. The big arrow indicate the MTR band.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MTR Antibody (NB100-791) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional MTR Products

Bioinformatics Tool for MTR Antibody (NB100-791)

Discover related pathways, diseases and genes to MTR Antibody (NB100-791). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MTR Antibody (NB100-791)

Discover more about diseases related to MTR Antibody (NB100-791).
 

Pathways for MTR Antibody (NB100-791)

View related products by pathway.

PTMs for MTR Antibody (NB100-791)

Learn more about PTMs related to MTR Antibody (NB100-791).
 

Research Areas for MTR Antibody (NB100-791)

Find related products by research area.

Blogs on MTR

There are no specific blogs for MTR, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
07/14/2012
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol MTR
Entrez
Uniprot