MTR Antibody

Western Blot: MTR Antibody [NB100-791] - Staining of Hela lysate (RIPA buffer, 1.4E5 cells per lane). Detected by chemiluminescence.

Immunohistochemistry-Paraffin: MTR Antibody [NB100-791] - Staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citratebuffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MTR Antibody Summary

• Immunogen: Peptide with sequence C-VEKWLGPILGYDTD corresponding to C-Terminus according to NP_000245.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MTR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 - 3 ug/ml
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Approx. 150 kDa band observed in HeLa and Jurkat lysates. IHC-P: Human adrenal gland shows cytoplasm staining in the cortex.
• Positive Control:

  Jurkat Lysate (NB800-PC2)
  HeLa Lysate (NB800-PC1)
  MTR Lysate (NBP2-66056)

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine. This batch is not expected to cross-react with Mouse, Rat and Pig Heart in Western blot.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MTR Antibody

• 5-methyltetrahydrofolate-homocysteine methyltransferase 1
• 5-methyltetrahydrofolate-homocysteine methyltransferase
• cblG
• cobalamin-dependent methionine synthase
• EC 2.1.1.13
• FLJ33168
• FLJ43216,5-methyltetrahydrofolate--homocysteine methyltransferase
• FLJ45386
• methionine synthase
• MS
• Vitamin-B12 dependent methionine synthase

Background

This enzyme catalyzes the final step in methionine biosynthesis. MTR mutations have been identified as an underlying cause of methylcobalamin deficiency complementation group G.Principle Names - MTR; 5-methyltetrahydrofolate-homocysteine methyltransferase methionine synthase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MTR Antibody (NB100-791)(2)

Publications using NB100-791

• Jiang H, Hurt KJ, Breen K et al. Sex-specific dysregulation of cysteine oxidation and the methionine and folate cycles in female cystathionine gamma-lyase null mice: a serendipitous model of the methylfolate trap. Biol Open. 2015 Aug 14 [PMID: 26276101] (WB, Mouse)
• Chen LH, Liu ML, Hwang HY et al. Human methionine synthase. cDNA cloning, gene localization, and expression. J Biol Chem 1997 Feb 7 [PMID: 9013615]

Review for MTR Antibody (NB100-791) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Anonymous WB Mouse 07/14/2012

Summary

• Application: Western Blot
• Sample Tested: mouse liver
• Species: Mouse
• Lot: P2
• Comments: This antibody is very good for WB using in mouse liver lysate. The big arrow indicate the MTR band.

Blocking

• Blocking Details: 5% milk in PBS pH 7.4, 0.2% tween 20

Primary Anitbody

• Dilution Ratio: 1:1000

Secondary Antibody

• Secondary Description: Rabbit anti-Goat IgG Fluorescein
• Secondary Manufacturer Cat#: 31509 from Thermo Sci.
• Secondary Concentration: 1:200

Details

• Detection Notes: Typhoon

Comments

• Comments: This antibody is very good for WB using in mouse liver lysate. The big arrow indicate the MTR band.

Bioinformatics

• Gene Symbol: MTR
• Entrez:
  • 4548(Human)
  • 81522(Rat)
• Uniprot:
  • Q99707(Human)