MTR Antibody

Western Blot: MTR Antibody [NB100-791] - Cgl null mice exhibit female-specific alteration of enzymes involved in the remethylation of methionine. Western blotting analysis of hepatic BHMT, MTHFR and MTR protein expression levels in male and female WT and Cgl null mice. Values shown represent the mean+-s.d., *Pless than 0.05, **Pless than 0.01, ***Pless than 0.001. Image collected and cropped by CiteAb from the following publication (//bio.biologists.org/cgi/doi/10.1242/bio.013433) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: MTR Antibody [NB100-791] - Human adrenal gland. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Po, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MTR Antibody Summary

• Immunogen: Peptide with sequence C-VEKWLGPILGYDTD corresponding to C-Terminus according to NP_000245.2.
• Predicted Species: Rat (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MTR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 5 ug/mL
  • Immunohistochemistry-Paraffin 3 - 5 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1-3 ug/ml
• Application Notes: Use in WB reported in scientific literature (PMID: 26276101).
• Control:

  Jurkat Whole Cell Lysate
  HeLa Whole Cell Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26276101).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MTR Antibody

• 5-methyltetrahydrofolate-homocysteine methyltransferase 1
• 5-methyltetrahydrofolate-homocysteine methyltransferase
• cblG
• cobalamin-dependent methionine synthase
• EC 2.1.1.13
• FLJ33168
• FLJ43216,5-methyltetrahydrofolate--homocysteine methyltransferase
• FLJ45386
• methionine synthase
• MS
• Vitamin-B12 dependent methionine synthase

Background

This enzyme catalyzes the final step in methionine biosynthesis. MTR mutations have been identified as an underlying cause of methylcobalamin deficiency complementation group G.Principle Names - MTR; 5-methyltetrahydrofolate-homocysteine methyltransferase methionine synthase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MTR Antibody (NB100-791)(3)

Publications using NB100-791

• Maclean KN, Jiang H, Phinney WN, et al. Derangement of hepatic polyamine, folate, and methionine cycle metabolism in cystathionine beta-synthase-deficient homocystinuria in the presence and absence of treatment: Possible implications for pathogenesis Molecular genetics and metabolism 2021-01-11 [PMID: 33483253]
• Jiang H, Hurt KJ, Breen K et al. Sex-specific dysregulation of cysteine oxidation and the methionine and folate cycles in female cystathionine gamma-lyase null mice: a serendipitous model of the methylfolate trap. Biol Open 2015-08-14 [PMID: 26276101] (WB, Mouse)
• Chen LH, Liu ML, Hwang HY et al. Human methionine synthase. cDNA cloning, gene localization, and expression. J Biol Chem 1997-02-07 [PMID: 9013615]

Reviews for MTR Antibody (NB100-791) (2)

Average Rating: 3

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Verified Customer Simple Western Human 01/10/2020

Summary

• Application: Simple Western
• Sample Tested: HepG2 CELLS
• Species: Human
• Lot: P2 E160819

Comments

• Comments: Low Rating Note: Novus Innovators Program - new species or application used on a primary antibody.

reviewed by: Hua Jiang WB Mouse 07/14/2012

Summary

• Application: Western Blot
• Sample Tested: mouse liver
• Species: Mouse
• Lot: P2

Bioinformatics

• Gene Symbol: MTR
• Entrez:
  • 4548(Human)
  • 81522(Rat)
• Uniprot:
  • Q99707(Human)