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MTR Antibody

 (2 Publications)
 (1 Review)
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Immunohistochemistry-Paraffin: MTR Antibody [NB100-791] - Paraffin embedded human adrenal gland. Antibody at 3.8 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: MTR Antibody [NB100-791] - Paraffin embedded human adrenal gland. Antibody at 3.8 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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MTR Antibody Summary

Immunogen
Peptide with sequence C-VEKWLGPILGYDTD corresponding to C-Terminus according to NP_000245.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MTR
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 150 kDa band observed in HeLa and Jurkat lysates. IHC-P: Human adrenal gland shows cytoplasm staining in the cortex.
Control
Jurkat Lysate (NB800-PC2)
HeLa Lysate (NB800-PC1)
MTR Lysate (NBP2-66056)
Reviewed Applications
Read 1 Review rated 5
using
NB100-791 in the following applications:
Publications
Read Publications using
NB100-791 in the following applications:

  • WB
    1 publication

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MTR Antibody

  • 5-methyltetrahydrofolate-homocysteine methyltransferase 1
  • 5-methyltetrahydrofolate-homocysteine methyltransferase
  • cblG
  • cobalamin-dependent methionine synthase
  • EC 2.1.1.13
  • FLJ33168
  • FLJ43216,5-methyltetrahydrofolate--homocysteine methyltransferase
  • FLJ45386
  • methionine synthase
  • MS
  • Vitamin-B12 dependent methionine synthase

Background

This enzyme catalyzes the final step in methionine biosynthesis. MTR mutations have been identified as an underlying cause of methylcobalamin deficiency complementation group G.Principle Names - MTR; 5-methyltetrahydrofolate-homocysteine methyltransferase methionine synthase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MTR Antibody (NB100-791)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Review for MTR Antibody (NB100-791) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-791:
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WB
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Images Ratings Applications Species Date Details
Western Blot MTR NB100-791
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reviewed by:
Anonymous
 WB Mouse 07/14/2012
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Summary

ApplicationWestern Blot
Sample Testedmouse liver
SpeciesMouse
LotP2
CommentsThis antibody is very good for WB using in mouse liver lysate. The big arrow indicate the MTR band.

Blocking

Blocking Details5% milk in PBS pH 7.4, 0.2% tween 20

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionRabbit anti-Goat IgG Fluorescein
Secondary Manufacturer Cat#31509 from Thermo Sci.
Secondary Concentration1:200

Details

Detection NotesTyphoon

Comments

CommentsThis antibody is very good for WB using in mouse liver lysate. The big arrow indicate the MTR band.

Product General Protocols

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FAQs for MTR Antibody (NB100-791) (0)

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Control Lysate(s)

Jurkat Whole Cell Lysate
HeLa Whole Cell Lysate
MTR Knockout 293T Cell Lysate

Secondary Antibodies

 

Isotype Controls

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Western Blot MTR NB100-791
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 Anonymous
07/14/2012
Application: WB
Species: Mouse
read this review

Bioinformatics

Gene Symbol MTR
Entrez
Uniprot