MTR Antibody

Images

 
Western Blot: MTR Antibody [NB100-791] - Cgl null mice exhibit female-specific alteration of enzymes involved in the remethylation of methionine. Western blotting analysis of hepatic BHMT, MTHFR and MTR protein ...read more
Immunohistochemistry-Paraffin: MTR Antibody [NB100-791] - Human adrenal gland. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, CaSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

MTR Antibody Summary

Immunogen
Peptide with sequence C-VEKWLGPILGYDTD corresponding to C-Terminus according to NP_000245.2.
Predicted Species
Rat (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MTR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Immunohistochemistry 3 - 5 ug/mL
  • Immunohistochemistry-Paraffin 3 - 5 ug/mL
  • Peptide ELISA Detection limit 1:32000
Application Notes
Use in WB reported in scientific literature (PMID: 26276101).
Control
Jurkat Whole Cell Lysate
HeLa Whole Cell Lysate
MTR Knockout 293T Cell Lysate
Reviewed Applications
Read 2 Reviews rated 3
using
NB100-791 in the following applications:

Publications
Read Publications using
NB100-791 in the following applications:

  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26276101).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MTR Antibody

  • 5-methyltetrahydrofolate-homocysteine methyltransferase 1
  • 5-methyltetrahydrofolate-homocysteine methyltransferase
  • cblG
  • cobalamin-dependent methionine synthase
  • EC 2.1.1.13
  • FLJ33168
  • FLJ43216,5-methyltetrahydrofolate--homocysteine methyltransferase
  • FLJ45386
  • methionine synthase
  • MS
  • Vitamin-B12 dependent methionine synthase

Background

This enzyme catalyzes the final step in methionine biosynthesis. MTR mutations have been identified as an underlying cause of methylcobalamin deficiency complementation group G.Principle Names - MTR; 5-methyltetrahydrofolate-homocysteine methyltransferase methionine synthase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MTR Antibody (NB100-791)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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WB
(1)
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Mouse
(1)
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Reviews for MTR Antibody (NB100-791) (2) 32

Average Rating: 3
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NB100-791:
Filter by Applications
Simple Western
(1)
WB
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western MTR NB100-791
Enlarge
1
reviewed by:
Jeremie RASO
Simple Western Human 01/10/2020
View

Summary

ApplicationSimple Western
Sample TestedHepG2 CELLS
SpeciesHuman
LotP2 E160819

Comments

CommentsLow Rating Note: Novus Innovators Program - new species or application used on a primary antibody.
Western Blot MTR NB100-791
Enlarge
5
reviewed by:
Anonymous
WB Mouse 07/14/2012
View

Summary

ApplicationWestern Blot
Sample Testedmouse liver
SpeciesMouse
LotP2
CommentsThis antibody is very good for WB using in mouse liver lysate. The big arrow indicate the MTR band.

Blocking

Blocking Details5% milk in PBS pH 7.4, 0.2% tween 20

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionRabbit anti-Goat IgG Fluorescein
Secondary Manufacturer Cat#31509 from Thermo Sci.
Secondary Concentration1:200

Details

Detection NotesTyphoon

Comments

CommentsThis antibody is very good for WB using in mouse liver lysate. The big arrow indicate the MTR band.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MTR Antibody (NB100-791) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for MTR Antibody (NB100-791)

Discover related pathways, diseases and genes to MTR Antibody (NB100-791). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for MTR Antibody (NB100-791)

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Pathways for MTR Antibody (NB100-791)

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Research Areas for MTR Antibody (NB100-791)

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Blogs on MTR

There are no specific blogs for MTR, but you can read our latest blog posts.
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Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

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Recent Reviews

3
5
1
4
0
3
0
2
0
1
1

Jeremie RASO
01/10/2020
Application: Simple Western
Species: Human

Anonymous
07/14/2012
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol MTR
Entrez
Uniprot