NHERF-2 Knockout 293T Cell Lysate

Product Discontinued

NHERF-2 Knockout 293T Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,11 bp deletion in exon 3
• Gene: SLC9A3R2

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout 293T Cell
• Life Stage: Embryonic
• Tissue: Kidney

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for NHERF-2 Knockout 293T Cell Lysate

• E3KARP
• Na(+)/H(+) exchange regulatory cofactor NHE-RF2
• NHE3RF2
• NHERF2MGC104639
• NHERF-2NHE3 kinase A regulatory protein E3KARP
• OCTS2
• SIP-1SRY-interacting protein 1
• sodium/hydrogen exchanger
• Sodium-hydrogen exchanger regulatory factor 2
• solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 2
• solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatoryfactor 2
• solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2
• Solute carrier family 9 isoform A3 regulatory factor 2
• TKA-1SIP1
• Tyrosine kinase activator protein 1

Background

This gene encodes a member of the NHERF family of PDZ scaffolding proteins. These proteins mediate many cellular processes by binding to and regulating the membrane expression and protein-protein interactions of membrane receptors and transport proteins. The encoded protein plays a role in intestinal sodium absorption by regulating the activity of the sodium/hydrogen exchanger 3, and may also regulate the cystic fibrosis transmembrane regulator (CFTR) ion channel. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: SLC9A3R2