NHERF-1 Antibody - BSA Free

Western Blot: NHERF-1 Antibody [NBP1-84918] - Western blot analysis in human cell line MCF-7.

Immunocytochemistry/Immunofluorescence: NHERF-1 Antibody [NBP1-84918] - Staining of human cell line A-431 shows localization to plasma membrane & microtubule organizing center. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: NHERF-1 Antibody [NBP1-84918] - Staining of human small intestine using Anti-SLC9A3R1 antibody NBP1-84918.

Immunohistochemistry-Paraffin: NHERF-1 Antibody [NBP1-84918] - Staining of human small intestine shows high expression.

Immunohistochemistry-Paraffin: NHERF-1 Antibody [NBP1-84918] - Staining in human small intestine and skeletal muscle tissues using anti-SLC9A3R1 antibody. Corresponding SLC9A3R1 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: NHERF-1 Antibody [NBP1-84918] - Staining of human kidney using Anti-SLC9A3R1 antibody.

Immunohistochemistry-Paraffin: NHERF-1 Antibody [NBP1-84918] - Staining of human endometrium using Anti-SLC9A3R1 antibody.

Immunohistochemistry-Paraffin: NHERF-1 Antibody [NBP1-84918] - Staining of human endometrium, kidney, skeletal muscle and small intestine using Anti-SLC9A3R1 antibody NBP1-84918 (A) shows similar protein distribution across tissues to independent antibody NBP1-84919 (B).

Immunohistochemistry-Paraffin: NHERF-1 Antibody [NBP1-84918] - Staining of human skeletal muscle using Anti-SLC9A3R1 antibody NBP1-84918.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NHERF-1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: GGDETKLLVVDRETDEFFKKCRVIPSQEHLNGPLPVPFTNGEIQKENSREALAEAALESPRPALVRSASSDTSEELNSQDSPPKQDSTAPSSTSSSDPILDFNISLAMAKERAHQKRSSKRAPQMDWSKKNELFSNL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NHERF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 0.04 - 0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  NHERF-1 Protein (NBP1-84918PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (88%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for NHERF-1 Antibody - BSA Free

• EBP50NHERF-1
• ezrin-radixin-moesin binding phosphoprotein-50
• Ezrin-radixin-moesin-binding phosphoprotein 50
• Na(+)/H(+) exchange regulatory cofactor NHE-RF1
• Na+/H+ exchange regulatory co-factor
• NHERF1solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1
• NHERFNPHLOP2
• Regulatory cofactor of Na(+)/H(+) exchanger
• sodium/hydrogen exchanger regulatory factor 1
• Sodium-hydrogen exchanger regulatory factor 1
• solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatoryfactor 1
• solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
• Solute carrier family 9 isoform A3 regulatory factor 1

Background

EBP 50 [ERM (ezrin-radixin-moesin) binding phosphoprotein of 50 kDa] is a PDZ containing protein that is involved in the linkage of integral membrane proteins to the cytoskeleton. EBP50 contains two tandem PDZ domains followed by a carboxy-terminal sequence that binds to members of the ERM family of membrane-cytoskeleton adaptors. The PDZ domains within EBP50 bind to the carboxy termini of such target proteins as beta2 adrenergic receptor, platelet-derived growth factor receptor (PDGFR), and the cystic fibrosis conductance regulator (CFTR). The PDZ domains are also believed to be involved in the oligomerization of EBP 50 to form multiprotein complexes which helps to facilitate the formation of functional signalling complexes. EBP 50 has been shown to link integral membrane proteins to the cytoskeleton by binding to members of the ERM family of proteins, which bind to F-actin. Through these interactions, EBP 50 is implicated in the localization of interactive groups of proteins into subcellular domains and in the regulation of activity of those interacting proteins. Immunohistochemical studies have shown that EBP 50 is found exclusively at the apical membranes of epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NHERF1